Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

10.3791/62391 ◽  
2021 ◽  
Author(s):  
Elizabeth A. Nelson ◽  
Jingyao Qiu ◽  
Nicholas W. Chavkin ◽  
Karen K. Hirschi
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Directed Differentiation

scholarly journals Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells

2017 ◽  
Vol 3 (11) ◽  
pp. e1701679 ◽  
Author(s):  
Tongcheng Qian ◽  
Shaenah E. Maguire ◽  
Scott G. Canfield ◽  
Xiaoping Bao ◽  
William R. Olson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Directed Differentiation ◽  
Blood Brain

scholarly journals Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

2017 ◽  
Vol 12 (9) ◽  
pp. 1890-1900 ◽  
Author(s):  
Xiaoping Bao ◽  
Xiaojun Lian ◽  
Tongcheng Qian ◽  
Vijesh J Bhute ◽  
Tianxiao Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Epicardial Cells ◽  
Term Maintenance

scholarly journals A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation

2015 ◽  
Vol 210 (7) ◽  
pp. 1257-1268 ◽  
Author(s):  
Sundari Chetty ◽  
Elise N. Engquist ◽  
Elie Mehanna ◽  
Kathy O. Lui ◽  
Alexander M. Tsankov ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Differentiation Potential ◽  
Germ Layers ◽  
Positive Effects ◽  
Src Inhibitor ◽  
Expression Of Genes

Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition of the cell cycle, activates proteins in the retinoblastoma family, and subsequently increases the differentiation propensities of hPSCs into all three germ layers. We further demonstrate that genetic suppression of Src regulates the activity of the retinoblastoma protein and enhances the differentiation potential of hPSCs across all germ layers. These positive effects extend beyond the initial germ layer specification and enable efficient differentiation at subsequent stages of differentiation.

Sign in / Sign up

Export Citation Format

Share Document