Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultra Force-Clamp Spectroscopy

10.3791/62388 ◽  
2021 ◽  
Author(s):  
L. Gardini ◽  
A. V. Kashchuk ◽  
F. S. Pavone ◽  
M. Capitanio
Keyword(s):  
Force Clamp

scholarly journals Ultrafast Force-Clamp Spectroscopy Reveals “Sliding” Catch-Bond Behavior of the Microtubule-Binding NdC80 Protein

2018 ◽  
Vol 114 (3) ◽  
pp. 382a ◽  
Author(s):  
Vladimir M. Demidov ◽  
Suvranta K. Tripathy ◽  
Fazly I. Ataullakhanov ◽  
Ekaterina L. Grishchuk
Keyword(s):  
Catch Bond ◽  
Microtubule Binding ◽  
Bond Behavior ◽  
Force Clamp

Single-molecule avidin–biotin association reaction studied by force-clamp spectroscopy

2008 ◽  
Vol 108 (10) ◽  
pp. 1135-1139 ◽  
Author(s):  
Mélanie Favre ◽  
Serguei K. Sekatskii ◽  
Giovanni Dietler
Keyword(s):  
Single Molecule ◽  
Association Reaction ◽  
Force Clamp

scholarly journals Dual-trap optical tweezers with real-time force clamp control

2011 ◽  
Vol 82 (8) ◽  
pp. 083102 ◽  
Author(s):  
Anders E. Wallin ◽  
Heikki Ojala ◽  
Gabija Ziedaite ◽  
Edward Hæggström
Keyword(s):  
Real Time ◽  
Optical Tweezers ◽  
Force Clamp

Nonuniform fatigue characteristics of slow-twitch motor units activated at a fixed percentage of their maximum tetanic tension

1991 ◽  
Vol 66 (5) ◽  
pp. 1483-1492 ◽  
Author(s):  
T. C. Cope ◽  
C. B. Webb ◽  
A. K. Yee ◽  
B. R. Botterman
Keyword(s):  
Relaxation Time ◽  
Conduction Velocity ◽  
Motor Units ◽  
Contraction Time ◽  
Stimulation Rate ◽  
Axonal Conduction ◽  
Slow Twitch ◽  
Force Clamp ◽  
Highly Correlated ◽  
Tetanic Tension

1. The endurance of slow-twitch motor units from the soleus (SOL) and medial gastrocnemius (MG) muscles of the cat were tested by determining the length of time (endurance time, Et) that a unit could maintain its tension output at 85% of maximum. Motor-unit tension was clamped at the target level by altering the stimulation rate of a unit's motor axon through computer feedback control. Tested in this way, units of both muscles displayed a wide range of Ets, approximately 40- to 50-fold. 2. Electromyographic (EMG) waveforms of motor units subjected to force-clamp contractions were analyzed to access whether any activity-dependent changes in their waveform shape might predict Et. Three measurements of waveform shape were determined: baseline-to-baseline duration, peak-to-peak amplitude, and area. Typically, amplitude decreased and duration increased as a contraction proceeded, whereas area remained fairly constant. Because changes in each measure were very similar for units of widely different Ets, it was concluded that neuromuscular junction failure and changes in the excitability of the sarcolemma (excluding the t-tubule system) play a minor role in determining Et. 3. Et was highly correlated with the mean stimulation rate (Et/number of stimuli) used during the force-clamp contractions. Mean rate was seen to progressively decrease with increasing Et. This correlation could not be explained by measures of isometric contractile speed or relaxation (e.g., twitch contraction time or half-relaxation time) measured before the force-clamp contractions. Both contraction time and half-relaxation time were found to be unrelated to both Et and the rate used to stimulate the unit during the force-clamp contraction. 4. Among type S units of SOL and MG, maximum tetanic tension and Et were not related. A significant relation (r = -0.49) was found between axonal conduction velocity and Et for SOL units (n = 38). In addition, a significant correlation (r = 0.47) was found between conduction velocity and tetanic tension for SOL units. Perhaps because of the small sample of type S units from MG (n = 10), conduction velocity was found not be related to either Et or tetanic tension. 5. Others have shown that a motor unit's maximum tetanic tension and axonal conduction velocity are correlated with its order of recruitment among motoneurons innervating a muscle. Recent work has further shown that among type F units the order in which a motoneuron is recruited is highly correlated with the fatigue resistance of its muscle unit.(ABSTRACT TRUNCATED AT 400 WORDS)

Single-Molecule Force-Clamp Experiments Reveal Kinetics of Mechanically Activated Silyl Ester Hydrolysis

ACS Nano ◽  
2012 ◽  
Vol 6 (2) ◽  
pp. 1314-1321 ◽  
Author(s):  
Sebastian W. Schmidt ◽  
Pavel Filippov ◽  
Alfred Kersch ◽  
Martin K. Beyer ◽  
Hauke Clausen-Schaumann
Keyword(s):  
Single Molecule ◽  
Ester Hydrolysis ◽  
Silyl Ester ◽  
Force Clamp ◽  
Kinetics Of

Measuring Microtubule Supertwist and Defects by Three-Dimensional-Force-Clamp Tracking of Single Kinesin-1 Motors

Nano Letters ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 1290-1295 ◽  
Author(s):  
Michael Bugiel ◽  
Aniruddha Mitra ◽  
Salvatore Girardo ◽  
Stefan Diez ◽  
Erik Schäffer
Keyword(s):  
Three Dimensional ◽  
Force Clamp

scholarly journals Magnetic Tweezers-Based Force Clamp Reveals Mechanically Distinct apCAM Domain Interactions

2012 ◽  
Vol 103 (6) ◽  
pp. 1120-1129 ◽  
Author(s):  
Devrim Kilinc ◽  
Agata Blasiak ◽  
James J. O’Mahony ◽  
Daniel M. Suter ◽  
Gil U. Lee
Keyword(s):  
Magnetic Tweezers ◽  
Domain Interactions ◽  
Force Clamp

scholarly journals Ultra-fast force-clamp spectroscopy data on the interaction between skeletal muscle myosin and actin

Data in Brief ◽  
2019 ◽  
Vol 25 ◽  
pp. 104017
Author(s):  
Manuela Maffei ◽  
Diego Beneventi ◽  
Monica Canepari ◽  
Roberto Bottinelli ◽  
Francesco Saverio Pavone ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Spectroscopy Data ◽  
Skeletal Muscle Myosin ◽  
Force Clamp ◽  
Muscle Myosin

scholarly journals Behavioral Thresholds for Force-Sensation Determined by an Integrated Video-Tracking and Force-Clamp System

2010 ◽  
Vol 98 (3) ◽  
pp. 192a
Author(s):  
Sung Jin Park ◽  
Bryan Petzold ◽  
Beth L. Pruitt ◽  
Miriam B. Goodman
Keyword(s):  
Video Tracking ◽  
Behavioral Thresholds ◽  
Force Clamp
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