Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

10.3791/62308 ◽  
2021 ◽  
Author(s):  
Samantha Ing-Esteves ◽  
Julie L. Lefebvre
Keyword(s):  
Time Lapse ◽  
Retinal Cell ◽  
Cell Populations ◽  
Adeno Associated Virus ◽  
Time Lapse Imaging ◽  
Neuronal Arborization

scholarly journals Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Claudia Prahst ◽  
Parham Ashrafzadeh ◽  
Thomas Mead ◽  
Ana Figueiredo ◽  
Karen Chang ◽  
...  
Keyword(s):  
Mouse Models ◽  
Fluorescent Microscopy ◽  
Light Sheet ◽  
Time Lapse ◽  
Confocal Imaging ◽  
Retinal Cell ◽  
Eye Disease ◽  
Cell Behaviour ◽  
Oxygen Induced Retinopathy ◽  
Time Lapse Imaging

As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated ‘knotted’ morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes.

scholarly journals Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

2019 ◽  
Author(s):  
Claudia Prahst ◽  
Parham Ashrafzadeh ◽  
Kyle Harrington ◽  
Lakshmi Venkatraman ◽  
Mark Richards ◽  
...  
Keyword(s):  
Mouse Models ◽  
Fluorescent Microscopy ◽  
Light Sheet ◽  
Time Lapse ◽  
Confocal Imaging ◽  
Retinal Cell ◽  
Eye Disease ◽  
Cell Behaviour ◽  
Oxygen Induced Retinopathy ◽  
Time Lapse Imaging

AbstractAs the general population ages and the incidence of diabetes increases epidemically, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the murine eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM) and 2) LSFM readily reveals new features of even well studied eye disease mouse models, such as the Oxygen-Induced Retinopathy (OIR) model. Through correlative LSFM-Confocal imaging we find that flat-mounting retinas for confocal microscopy significantly distorts tissue morphology. The minimized distortion with LSFM dramatically improved analysis of pathological vascular tufts in the OIR model revealing “knotted” morphologies, leading to a proposed new tuft nomenclature. Furthermore, live-imaging of OIR tuft formation revealed abnormal cell motility and altered filopodia dynamics. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of pathological processes in the eye, in particular neuro-vascular degenerative processes.

Cleavage of Human Embryos: Options and Diversity

Acta Naturae ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 88-96
Author(s):  
Yu. K. Doronin ◽  
I. V. Senechkin ◽  
L. V. Hilkevich ◽  
M. A. Kurcer
Keyword(s):  
Time Lapse ◽  
Reproductive Technologies ◽  
Human Embryos ◽  
Imaging Data ◽  
Noninvasive Methods ◽  
Topographic Features ◽  
Time Parameters ◽  
Embryo Cleavage ◽  
Time Lapse Imaging ◽  
Developmental Dynamics

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

scholarly journals Evaluating the utility of time-lapse imaging in the estimation of post-mortem interval: An Australian case study

2019 ◽  
Vol 1 ◽  
pp. 204-210 ◽  
Author(s):  
Alyson Wilson ◽  
Stanley Serafin ◽  
Dilan Seckiner ◽  
Rachel Berry ◽  
Xanthé Mallett
Keyword(s):  
Time Lapse ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Australian Case ◽  
Time Lapse Imaging

scholarly journals Time-lapse imaging of CO2 migration within near-surface sediments during a controlled sub-seabed release experiment

2021 ◽  
Vol 109 ◽  
pp. 103363
Author(s):  
Ben Roche ◽  
Jonathan M. Bull ◽  
Hector Marin-Moreno ◽  
Timothy G. Leighton ◽  
Ismael H. Falcon-Suarez ◽  
...  
Keyword(s):  
Surface Sediments ◽  
Time Lapse ◽  
Near Surface ◽  
Release Experiment ◽  
Time Lapse Imaging

Segmentation and Track-Analysis in Time-Lapse Imaging of Bacteria

2016 ◽  
Vol 10 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Sajith Kecheril Sadanandan ◽  
Ozden Baltekin ◽  
Klas E. G. Magnusson ◽  
Alexis Boucharin ◽  
Petter Ranefall ◽  
...  
Keyword(s):  
Time Lapse ◽  
Time Lapse Imaging ◽  
Track Analysis

scholarly journals Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

2017 ◽  
Vol 36 (5) ◽  
pp. 519-528 ◽  
Author(s):  
Tomoyo Tanaka ◽  
Mitsuhiro Hoshijima ◽  
Junko Sunaga ◽  
Takashi Nishida ◽  
Mana Hashimoto ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Time Lapse ◽  
Living Bone ◽  
Time Lapse Imaging
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