Assessing Biofilm Dispersal in Murine Wounds

10.3791/62136 ◽  
2021 ◽  
Author(s):  
Whitni K. Redman ◽  
Garrett S. Welch ◽  
Kendra P. Rumbaugh
Keyword(s):  
Biofilm Dispersal

scholarly journals Acceleration of protease effect on Staphylococcus aureus biofilm dispersal

2012 ◽  
Vol 335 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Joo-Hyeon Park ◽  
Jin-Hyung Lee ◽  
Moo Hwan Cho ◽  
Moshe Herzberg ◽  
Jintae Lee
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Dispersal

Endogenous nitric oxide-generating surfaces via polydopamine-copper coatings for preventing biofilm dispersal and promoting microbial killing

2021 ◽  
pp. 112297
Author(s):  
Jayanti Mendhi ◽  
Srinivas Sulugodu Ramachandra ◽  
Indira Prasadam ◽  
Saso Ivanovski ◽  
Ying Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Copper Coatings ◽  
Endogenous Nitric Oxide ◽  
Biofilm Dispersal

scholarly journals Biofilm dispersal: multiple elaborate strategies for dissemination of bacteria with unique properties

2017 ◽  
Vol 105 (2) ◽  
pp. 188-210 ◽  
Author(s):  
Cyril Guilhen ◽  
Christiane Forestier ◽  
Damien Balestrino
Keyword(s):  
Biofilm Dispersal

scholarly journals Anionic amino acids support hydrolysis of poly-β-(1,6)-N-acetylglucosamine exopolysaccharides by the biofilm dispersing glycosidase Dispersin B

2020 ◽  
pp. jbc.RA120.015524
Author(s):  
Alexandra P Breslawec ◽  
Shaochi Wang ◽  
Crystal Li ◽  
Myles B Poulin
Keyword(s):  
Amino Acids ◽  
Bacterial Infections ◽  
Substrate Recognition ◽  
Site Directed Mutagenesis ◽  
Binding Surface ◽  
Activity Measurements ◽  
Rate Of Hydrolysis ◽  
Biofilm Dispersal ◽  
Hydrolysis Of

The exopolysaccharide poly-β-(1→6)-N-acetylglucosamine (PNAG) is a major structural determinant of bacterial biofilms responsible for persistent and nosocomial infections. The enzymatic dispersal of biofilms by PNAG-hydrolyzing glycosidase enzymes, such as Dispersin B (DspB), is a possible approach to treat biofilm dependent bacterial infections. The cationic charge resulting from partial de-N-acetylation of native PNAG is critical for PNAG-dependent biofilm formation. We recently demonstrated that DspB has increased catalytic activity on de-N-acetylated PNAG oligosaccharides, but the molecular basis for this increased activity is not known. Here, we analyze the role of anionic amino acids surrounding the catalytic pocket of DspB in PNAG substrate recognition and hydrolysis using a combination of site directed mutagenesis, activity measurements using synthetic PNAG oligosaccharide analogs, and in vitro biofilm dispersal assays. The results of these studies support a model in which bound PNAG is weakly associated with a shallow anionic groove on the DspB protein surface with recognition driven by interactions with the –1 GlcNAc residue in the catalytic pocket. An increased rate of hydrolysis for cationic PNAG was driven, in part, by interaction with D147 on the anionic surface. Moreover, we identified that a DspB mutant with improved hydrolysis of fully acetylated PNAG oligosaccharides correlates with improved in vitro dispersal of PNAG dependent Staphylococcus epidermidis biofilms. These results provide insight into the mechanism of substrate recognition by DspB and suggest a method to improve DspB biofilm dispersal activity by mutation of the amino acids within the anionic binding surface.

scholarly journals Differential impact on motility and biofilm dispersal of closely related phosphodiesterases in Pseudomonas aeruginosa

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu-ming Cai ◽  
Andrew Hutchin ◽  
Jack Craddock ◽  
Martin A. Walsh ◽  
Jeremy S. Webb ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Differential Impact ◽  
Biofilm Dispersal

scholarly journals Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

2019 ◽  
Vol 64 (1) ◽  
Author(s):  
Anthony D. Verderosa ◽  
Rabeb Dhouib ◽  
Kathryn E. Fairfull-Smith ◽  
Makrina Totsika
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Biofilms ◽  
Gram Positive ◽  
Gram Negative ◽  
Content Type ◽  
Microscopy Analysis ◽  
Biofilm Dispersal ◽  
Biofilm Structure ◽  
Dispersal Activity

ABSTRACT Treatment of biofilm-related Staphylococcus aureus infections represents an important medical challenge worldwide, as biofilms, even those involving drug-susceptible S. aureus strains, are highly refractory to conventional antibiotic therapy. Nitroxides were recently shown to induce the dispersal of Gram-negative biofilms in vitro, but their action against Gram-positive bacterial biofilms remains unknown. Here, we demonstrate that the biofilm dispersal activity of nitroxides extends to S. aureus, a clinically important Gram-positive pathogen. Coadministration of the nitroxide CTEMPO (4-carboxy-2,2,6,6-tetramethylpiperidin-1-yloxyl) with ciprofloxacin significantly improved the biofilm eradication activity of the antibiotic against S. aureus. Moreover, covalently linking the nitroxide to the antibiotic moiety further reduced the ciprofloxacin minimal biofilm eradication concentration. Microscopy analysis revealed that fluorescent nitroxide-antibiotic hybrids could penetrate S. aureus biofilms and enter cells localized at the surface and base of the biofilm structure. No toxicity to human cells was observed for the nitroxide CTEMPO or the nitroxide-antibiotic hybrids. Taken together, our results show that nitroxides can mediate the dispersal of Gram-positive biofilms and that dual-acting biofilm eradication antibiotics may provide broad-spectrum therapies for the treatment of biofilm-related infections.

Sign in / Sign up

Export Citation Format

Share Document