High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures

10.3791/61988 ◽  
2021 ◽  
Author(s):  
Vito Antonio Baldassarro
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Cultures ◽  
Precursor Cell ◽  
High Content Screening ◽  
Oligodendrocyte Precursor Cell ◽  
Adult Neural Stem Cell ◽  
Oligodendrocyte Precursor

Olanzapine stimulates proliferation but inhibits differentiation in rat oligodendrocyte precursor cell cultures

2011 ◽  
Vol 35 (8) ◽  
pp. 1950-1956 ◽  
Author(s):  
Sohei Kimoto ◽  
Aya Okuda ◽  
Michihiro Toritsuka ◽  
Takahira Yamauchi ◽  
Manabu Makinodan ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Precursor Cell ◽  
Oligodendrocyte Precursor Cell ◽  
Oligodendrocyte Precursor

A xeno-free culturing protocol for pluripotent stem cell-derived oligodendrocyte precursor cell production

2011 ◽  
Vol 6 (4) ◽  
pp. 449-460 ◽  
Author(s):  
Maria Sundberg ◽  
Anu Hyysalo ◽  
Heli Skottman ◽  
Soojung Shin ◽  
Mohan Vemuri ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Precursor Cell ◽  
Oligodendrocyte Precursor Cell ◽  
Cell Production ◽  
Oligodendrocyte Precursor

Ginkgolide B promotes oligodendrocyte precursor cell differentiation and survival via Akt/CREB/bcl-2 signaling pathway after white matter lesion

2021 ◽  
pp. 153537022198995
Author(s):  
Jian Huang ◽  
Jun Yang ◽  
Xingju Zou ◽  
Shilun Zuo ◽  
Jing Wang ◽  
...  
Keyword(s):  
White Matter ◽  
White Matter Lesion ◽  
Neuroprotective Effect ◽  
Precursor Cell ◽  
Cerebral Hypoperfusion ◽  
Ginkgolide B ◽  
Oligodendrocyte Precursor Cell ◽  
Learning Abilities ◽  
Phosphorylated Akt ◽  
Oligodendrocyte Precursor

White matter lesion (WML) is caused by chronic cerebral hypoperfusion, which are usually associated with cognitive impairment. Evidence from recent studies has shown that ginkgolide B has a neuroprotective effect that could be beneficial for the treatment of ischemia; however, it is not clear whether ginkgolide B has a protective effect on WML. Our data show that ginkgolide B can promote the differentiation of oligodendrocyte precursor cell (OPC) into oligodendrocytes and promote oligodendrocyte survival following a WML. Ginkgolide B (5, 10, 20 mg/kg) or saline is administered intraperitoneally every day after WML. After 4 weeks, the data of Morris water maze suggested that rats’ memory and learning abilities were impaired, and the administration of ginkgolide B enhanced behavioral achievement. Also, treatment with ginkgolide B significantly attenuated this loss of myelin. Our result suggests that ginkgolide B promotes the differentiation of OPC into oligodendrocytes. We also found that ginkgolide B ameliorates oligodendrocytes apoptosis. Furthermore, ginkgolide B enhanced the expression of phosphorylated Akt and CREB. In conclusion, our data firstly show that ginkgolide B promotes oligodendrocyte genesis and oligodendrocyte myelin following a WML, possibly involving the Akt and CREB pathways.

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