scholarly journals Cryo-injury Induced Heart Regeneration in the Axolotl and Echocardiography and Unbiased Quantitative Histology to Evaluate Regenerative Progression

10.3791/61966 ◽  
2021 ◽  
Author(s):  
Anita Dittrich ◽  
Henrik Lauridsen
Keyword(s):  
Heart Regeneration ◽  
Quantitative Histology

Stem Cell-Based Therapies for Heart Regeneration: What did the Bench Teach Us?

2010 ◽  
Vol 10 (3) ◽  
pp. 173-185 ◽  
Author(s):  
L. Ferreira ◽  
D. C.S. Pedroso ◽  
H. Vazao ◽  
R. S.M. Gomes
Keyword(s):  
Stem Cell ◽  
Heart Regeneration

scholarly journals Protocol for cryoinjury model in neonatal mice for heart regeneration and repair research

2021 ◽  
Vol 2 (3) ◽  
pp. 100623
Author(s):  
Yanli Zhao ◽  
Rong Chang ◽  
Changchun Zeng
Keyword(s):  
Heart Regeneration ◽  
Neonatal Mice

scholarly journals Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

2019 ◽  
Vol 6 (2) ◽  
pp. 16 ◽  
Author(s):  
Suneeta Narumanchi ◽  
Karri Kalervo ◽  
Sanni Perttunen ◽  
Hong Wang ◽  
Katariina Immonen ◽  
...  
Keyword(s):  
Cardiac Function ◽  
Cardiac Regeneration ◽  
Nuclear Antigen ◽  
Heart Regeneration ◽  
Adult Zebrafish ◽  
Tissue Samples ◽  
Micro Rnas ◽  
Proliferating Cell ◽  
Zebrafish Heart

The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.

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