scholarly journals Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

10.3791/61863 ◽  
2020 ◽  
Author(s):  
María Portela-Lomba ◽  
Diana Simón ◽  
Cristina Russo ◽  
Javier Sierra ◽  
María Teresa Moreno-Flores
Keyword(s):  
Axonal Regeneration ◽  
In Vitro Model ◽  
Retinal Ganglion ◽  
Olfactory Ensheathing Glia ◽  
Vitro Model ◽  
Ganglion Neurons ◽  
Retinal Ganglion Neurons

scholarly journals Immortalized olfactory ensheathing glia promote axonal regeneration of rat retinal ganglion neurons

2003 ◽  
Vol 85 (4) ◽  
pp. 861-871 ◽  
Author(s):  
M. Teresa Moreno-Flores ◽  
Filip Lim ◽  
M. Jesús Martín-Bermejo ◽  
Javier Díaz-Nido ◽  
Jesús Ávila ◽  
...  
Keyword(s):  
Axonal Regeneration ◽  
Retinal Ganglion ◽  
Olfactory Ensheathing Glia ◽  
Ganglion Neurons ◽  
Retinal Ganglion Neurons

scholarly journals THE CHANGES IN DISTRIBUTION OF NMDA- AND AMPA-RECEPTORS IN RETINOCOLLICULAR SYNAPSES

2021 ◽  
Vol 67 (3) ◽  
pp. 10-16
Author(s):  
H.V. Dumanska ◽  
◽  
O.V. Rikchalsky ◽  
N.S. Veselovsky ◽  
◽  
...  
Keyword(s):  
Ampa Receptors ◽  
In Vitro Model ◽  
Ganglion Cells ◽  
Signal Transmission ◽  
Main Role ◽  
Retinal Ganglion ◽  
Dynamic Changes ◽  
Functional Roles ◽  
Vitro Model

We investigated the changes in distribution of the NMDA- and AMPA-receptors in the synapses in the in vitro model of the retinocollicular pathway. The model was a primary coculture of the retinal cells and superficial superior colliculus (SSC) neurons. Evoked postsynaptic currents (ePSCs) were recorded in SSC neurons in response to local electrical extracellular stimulation of the afferent retinal ganglion cells’ (RGC) axons. We analyzed the changes in the kinetic characteristics of ePSCs at different holding potentials during cocultivation. The results obtained reflect that NMDA receptors play an essential role in the formation of the retinocollicular synapses. After the formation of retinocollicular connections, the main role in the sensory signal transmission belongs to AMPA receptors. Thus, the data obtained indicate the specific dynamic changes of the functional roles of NMDA- and AMPA-receptors in the forma- tion and development of the retinocollicular synaptic contacts.

Patient-derived olfactory mucosa cells but not lung or skin fibroblasts mediate axonal regeneration of retinal ganglion neurons

2012 ◽  
Vol 509 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Vega García-Escudero ◽  
Ana García-Gómez ◽  
Elena Langa ◽  
María Jesús Martín-Bermejo ◽  
Rafael Ramírez-Camacho ◽  
...  
Keyword(s):  
Axonal Regeneration ◽  
Olfactory Mucosa ◽  
Skin Fibroblasts ◽  
Retinal Ganglion ◽  
Ganglion Neurons ◽  
Retinal Ganglion Neurons

scholarly journals Dorsal Root Ganglion Neurons Carrying a P301S Tau Mutation: A Valid In Vitro Model for Screening Drugs against Tauopathies?

2014 ◽  
Vol 34 (14) ◽  
pp. 4757-4759 ◽  
Author(s):  
Niels Alberts ◽  
Karlijn Groen ◽  
Lisette Klein ◽  
Marek J. Konieczny ◽  
Mandy Koopman
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Dorsal Root Ganglion Neurons ◽  
Vitro Model ◽  
Ganglion Neurons ◽  
Tau Mutation

Retinal ganglion neurons express a toxin-resistant developmentally regulated novel type of high-voltage-activated calcium channel

1994 ◽  
Vol 72 (5) ◽  
pp. 2542-2546 ◽  
Author(s):  
T. Rothe ◽  
R. Grantyn
Keyword(s):  
High Voltage ◽  
Channel Blocker ◽  
Retinal Ganglion ◽  
The Novel ◽  
Developmentally Regulated ◽  
Electrophysiological Measurements ◽  
Ganglion Neurons ◽  
Retinal Ganglion Neurons ◽  
Developmental Analysis

1. High-voltage-activated Ca2+ currents [ICa(HVA)] were studied in immunolabeled mouse retinal ganglion neurons (RGNs) to elucidate channel-specific components and their developmental changes in vitro. 2. Neurons were dissociated at postnatal day 5. RGNs were selected for electrophysiological measurements by vital labeling with an antibody against Thy-1.2. ICa(HVA) were recorded with patch electrodes in the whole cell configuration at a holding voltage (Vh) of -90 mV. 3. A total of 111 neurons was studied. On average, 13% of ICa(HVA) was reversibly blocked by 10 microM nifedipine, approximately 30% of the compound current displayed an irreversible block by 2.5 microM omega-conotoxin (omega-CTX) GVIA. The remainder current was resistant to both drugs, suggesting that the total ICa(HVA) was a mixture of at least three different components. 4. Developmental analysis revealed a significant increase of the omega-CTX-GVIA/nifedipine-resistant component of ICa(HVA) (31% at day in vitro (DIV) 0–2, 70% at DIV 18–26) mainly at the expense of the omega-CTX-GVIA-sensitive current. No significant change was found in the nifedipine-sensitive component of ICa(HVA). 5. To characterize the Ca2+ current component that was resistant to both omega-CTX-GVIA and nifedipine at Vh -90 mV, three tests were performed. The P channel antagonist omega-agatoxin IVA (omega-Aga-IVA, 200 nM) completely failed to block ICa(HVA) in mouse RGNs. The novel Ca2+ channel blocker omega-CTX-MVIIC (5 microM) decreased the ICa(HVA) remaining after omega-CTX-GVIA treatment by only approximately 10%.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Differentiated neuroblastoma F-11 cells as an alternative in-vitro model to dorsal root ganglion neurons

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Stefania Blasa ◽  
Valentina Pastori ◽  
Alessia D’Aloia ◽  
Marzia M. Lecchi
Keyword(s):  
Animal Models ◽  
Dorsal Root Ganglion ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Dorsal Root Ganglion Neurons ◽  
Biomedical Field ◽  
Vitro Model ◽  
Ganglion Neurons

We induced differentiation in F-11 cells to verify if they could show similarities with sensory neurons, in order to develop an alternative to animal models for research studies in the biomedical field.

scholarly journals Neuroprotective and neuroregenerative effects of CRMP-5 on retinal ganglion cells in an experimental in vivo and in vitro model of glaucoma

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0207190 ◽  
Author(s):  
Jasmin Lauzi ◽  
Fabian Anders ◽  
Hanhan Liu ◽  
Norbert Pfeiffer ◽  
Franz Grus ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
In Vitro Model ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Vitro Model
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