Measurement of Strial Blood Flow in Mouse Cochlea utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

10.3791/61857 ◽  
2021 ◽  
Author(s):  
Zhiqiang Hou ◽  
Yunpei Zhang ◽  
Lingling Neng ◽  
Jinhui Zhang ◽  
Xiaorui Shi
Keyword(s):  
Blood Flow ◽  
Fluorescence Microscopy ◽  
Intravital Fluorescence Microscopy

Measurement of cochlear blood flow: Intravital fluorescence microscopy

1989 ◽  
Vol 42 (2-3) ◽  
pp. 229-236 ◽  
Author(s):  
Jiri Prazma ◽  
Vincent N. Carrasco ◽  
C.Gaelyn Garrett ◽  
Harold C. Pillsbury
Keyword(s):  
Blood Flow ◽  
Fluorescence Microscopy ◽  
Intravital Fluorescence Microscopy ◽  
Cochlear Blood Flow

Characterization and prevention of phototoxic effects in intravital fluorescence microscopy in the hamster dorsal skinfold model

2000 ◽  
Vol 385 (4) ◽  
pp. 290-298 ◽  
Author(s):  
M. Steinbauer ◽  
Anthony Gustav Harris ◽  
Christoph Abels ◽  
Konrad Messmer
Keyword(s):  
Fluorescence Microscopy ◽  
Intravital Fluorescence Microscopy

scholarly journals Perfusion heterogeneity in the pulmonary acinus

1998 ◽  
Vol 84 (3) ◽  
pp. 933-938 ◽  
Author(s):  
Nobuhiro Tanabe ◽  
Thomas M. Todoran ◽  
Gerald M. Zenk ◽  
Brenda R. Bunton ◽  
Wiltz W. Wagner ◽  
...  
Keyword(s):  
Blood Flow ◽  
Fluorescence Microscopy ◽  
Capillary Blood ◽  
Fluorescent Dye ◽  
Time Difference ◽  
Appearance Time ◽  
Pulmonary Acinus ◽  
The Mean ◽  
The Difference ◽  
Dilution Curves

There is little information on the distribution of acinar perfusion because it is difficult to resolve blood flow within such small regions. We hypothesized that the known heterogeneity of arteriolar blood flow and capillary blood flow would result in heterogeneous acinar perfusion. To test this hypothesis, the passage of fluorescent dye boluses through the subpleural microcirculation of isolated dog lobes was videotaped by using fluorescence microscopy. As the videotapes were replayed, dye-dilution curves were recorded from each of the tributary branches of Y-shaped venules that drained an acinus. From the dye curves, we calculated the mean appearance time of each curve. The difference in mean appearance times between venular tributary branches was small in most cases. In 43% of the observed venular branch pairs, the dye curves were essentially superimposable (the mean appearance-time difference was <5%); and in another 42%, the mean appearance-time difference between curves was 5–10%. From these results, we conclude that acinar perfusion is unexpectedly homogeneous.

scholarly journals Quantitative Assessment of Angiogenesis in Murine Antigen-Induced Arthritis by Intravital Fluorescence Microscopy

2003 ◽  
Vol 40 (5) ◽  
pp. 460-466 ◽  
Author(s):  
M. Schmitt-Sody ◽  
J. Landes ◽  
S.P. Zysk ◽  
C. Pellengahr ◽  
F. Krombach ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Quantitative Assessment ◽  
Intravital Fluorescence Microscopy

scholarly journals In vivo two-photon excited fluorescence microscopy reveals cardiac- and respiration-dependent pulsatile blood flow in cortical blood vessels in mice

2012 ◽  
Vol 302 (7) ◽  
pp. H1367-H1377 ◽  
Author(s):  
Thom P. Santisakultarm ◽  
Nathan R. Cornelius ◽  
Nozomi Nishimura ◽  
Andrew I. Schafer ◽  
Richard T. Silver ◽  
...  
Keyword(s):  
Blood Flow ◽  
Fluorescence Microscopy ◽  
Capillary Tube ◽  
Three Dimensional ◽  
Vessel Diameter ◽  
Vascular Network ◽  
Maximum Speed ◽  
Flow Profile ◽  
Two Photon ◽  
Surface Vessels

Subtle alterations in cerebral blood flow can impact the health and function of brain cells and are linked to cognitive decline and dementia. To understand hemodynamics in the three-dimensional vascular network of the cerebral cortex, we applied two-photon excited fluorescence microscopy to measure the motion of red blood cells (RBCs) in individual microvessels throughout the vascular hierarchy in anesthetized mice. To resolve heartbeat- and respiration-dependent flow dynamics, we simultaneously recorded the electrocardiogram and respiratory waveform. We found that centerline RBC speed decreased with decreasing vessel diameter in arterioles, slowed further through the capillary bed, and then increased with increasing vessel diameter in venules. RBC flow was pulsatile in nearly all cortical vessels, including capillaries and venules. Heartbeat-induced speed modulation decreased through the vascular network, while the delay between heartbeat and the time of maximum speed increased. Capillary tube hematocrit was 0.21 and did not vary with centerline RBC speed or topological position. Spatial RBC flow profiles in surface vessels were blunted compared with a parabola and could be measured at vascular junctions. Finally, we observed a transient decrease in RBC speed in surface vessels before inspiration. In conclusion, we developed an approach to study detailed characteristics of RBC flow in the three-dimensional cortical vasculature, including quantification of fluctuations in centerline RBC speed due to cardiac and respiratory rhythms and flow profile measurements. These methods and the quantitative data on basal cerebral hemodynamics open the door to studies of the normal and diseased-state cerebral microcirculation.

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