scholarly journals High-throughput and Deep-proteome Profiling by 16-plex Tandem Mass Tag Labeling Coupled with Two-dimensional Chromatography and Mass Spectrometry

10.3791/61684 ◽  
2020 ◽  
Author(s):  
Zhen Wang ◽  
Kanisha Kavdia ◽  
Kaushik Kumar Dey ◽  
Vishwajeeth Reddy Pagala ◽  
Kiran Kodali ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Proteome Profiling ◽  
Tandem Mass Tag ◽  
Mass Tag

scholarly journals Deep Profiling of Microgram-Scale Proteome by Tandem Mass Tag Mass Spectrometry

2020 ◽  
Vol 20 (1) ◽  
pp. 337-345
Author(s):  
Danting Liu ◽  
Shu Yang ◽  
Kanisha Kavdia ◽  
Jeffrey M. Sifford ◽  
Zhiping Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass ◽  
Tandem Mass Tag ◽  
Mass Tag

scholarly journals Bait Correlation Improves Interactor Identification by Tandem Mass Tag-Affinity Purification-Mass Spectrometry

2020 ◽  
Vol 19 (4) ◽  
pp. 1565-1573
Author(s):  
Liangyong Mei ◽  
Maureen R. Montoya ◽  
Guy M. Quanrud ◽  
Minh Tran ◽  
Athena Villa-Sharma ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Affinity Purification ◽  
Tandem Mass ◽  
Tandem Mass Tag ◽  
Affinity Purification Mass Spectrometry ◽  
Mass Tag

scholarly journals Differential Proteomics Based on TMT and PRM Reveal the Resistance Response of Bambusa pervariabilis × Dendrocalamopisis grandis Induced by AP-Toxin

Metabolites ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 166 ◽  
Author(s):  
Qianqian He ◽  
Xinmei Fang ◽  
Tianhui Zhu ◽  
Shan Han ◽  
Hanmingyue Zhu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Metabolic Pathways ◽  
Differentially Expressed ◽  
Tandem Mass ◽  
Differentially Expressed Proteins ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Tandem Mass Tag ◽  
Mass Tag

Bambusa pervariabilis McClure × Dendrocalamopsis grandis (Q.H.Dai & X.l.Tao ex Keng f.) Ohrnb. blight is a widespread and dangerous forest fungus disease, and has been listed as a supplementary object of forest phytosanitary measures. In order to study the control of B. pervariabilis × D. grandis blight, this experiment was carried out. In this work, a toxin purified from the pathogen Arthrinium phaeospermum (Corda) Elli, which causes blight in B. pervariabilis × D. grandis, with homologous heterogeneity, was used as an inducer to increase resistance to B. pervariabilis × D. grandis. A functional analysis of the differentially expressed proteins after induction using a tandem mass tag labeling technique was combined with mass spectrometry and liquid chromatography mass spectrometry in order to effectively screen for the proteins related to the resistance of B. pervariabilis × D. grandis to blight. After peptide labeling, a total of 3320 unique peptides and 1791 quantitative proteins were obtained by liquid chromatography mass spectrometry analysis. Annotation and enrichment analysis of these peptides and proteins using the Gene ontology and Kyoto Encyclopedia of Genes and Genomes databases with bioinformatics software show that the differentially expressed protein functional annotation items are mainly concentrated on biological processes and cell components. Several pathways that are prominent in the Kyoto Encyclopedia of Genes and Genomes annotation and enrichment include metabolic pathways, the citrate cycle, and phenylpropanoid biosynthesis. In the Protein-protein interaction networks four differentially expressed proteins-sucrose synthase, adenosine triphosphate-citrate synthase beta chain protein 1, peroxidase, and phenylalanine ammonia-lyase significantly interact with multiple proteins and significantly enrich metabolic pathways. To verify the results of tandem mass tag, the candidate proteins were further verified by parallel reaction monitoring, and the results were consistent with the tandem mass tag data analysis results. It is confirmed that the data obtained by tandem mass tag technology are reliable. Therefore, the differentially expressed proteins and signaling pathways discovered here is the primary concern for subsequent disease resistance studies.

Automating mass spectrometry-based quantitative glycomics using aminoxy tandem mass tag reagents with SimGlycan

2015 ◽  
Vol 127 ◽  
pp. 211-222 ◽  
Author(s):  
Ningombam Sanjib Meitei ◽  
Arun Apte ◽  
Sergei I. Snovida ◽  
John C. Rogers ◽  
Julian Saba
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass ◽  
Tandem Mass Tag ◽  
Mass Tag

scholarly journals Comparative proteomics of salivary glands of Anopheles culicifacies mosquitoes using tandem mass tag (TMT) mass spectrometry

2018 ◽  
Vol 55 (2) ◽  
pp. 98 ◽  
Author(s):  
Arun Sharma ◽  
Ritu Rawal ◽  
Sonam Vijay ◽  
Kavita Kadian ◽  
Tridibesh Adak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Salivary Glands ◽  
Comparative Proteomics ◽  
Tandem Mass ◽  
Anopheles Culicifacies ◽  
Tandem Mass Tag ◽  
Mass Tag

scholarly journals Tandem Mass Tag-Based Serum Proteome Profiling for Biomarker Discovery in Young Duchenne Muscular Dystrophy Boys

ACS Omega ◽  
2020 ◽  
Vol 5 (41) ◽  
pp. 26504-26517
Author(s):  
Tchilabalo D. Alayi ◽  
Shefa M. Tawalbeh ◽  
Michael Ogundele ◽  
Holly R. Smith ◽  
Alison M. Samsel ◽  
...  
Keyword(s):  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Biomarker Discovery ◽  
Tandem Mass ◽  
Proteome Profiling ◽  
Serum Proteome ◽  
Tandem Mass Tag ◽  
Mass Tag
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