Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

10.3791/61579 ◽  
2020 ◽  
Author(s):  
Yangyang Deng ◽  
Ming Zhou ◽  
Ying Lu ◽  
Jiao Chen ◽  
Zuhui Pu ◽  
...  
Keyword(s):  
Xenotransplantation Model

Mean survival of 22 days in a worldwide first heterotopic thoracic pig-to-baboon cardiac xenotransplantation model

2013 ◽  
Vol 61 (S 01) ◽  
Author(s):  
S Buchholz ◽  
J Abicht ◽  
T Mayr ◽  
J Postrach ◽  
S Güthoff ◽  
...  
Keyword(s):  
Xenotransplantation Model

Costimulation Blockade in a Heterotopic Thoracic Pig-to-baboon Cardiac Xenotransplantation Model

2015 ◽  
Vol 63 (S 01) ◽  
Author(s):  
J.-M. Abicht ◽  
T. Mayr ◽  
S. Guethoff ◽  
F. Werner ◽  
I. Lutzmann ◽  
...  
Keyword(s):  
Costimulation Blockade ◽  
Xenotransplantation Model

Graves' disease: xenotransplantation model (athymic nude mice)

1999 ◽  
Vol 77 (1) ◽  
pp. 185-188 ◽  
Author(s):  
K. Jungheim ◽  
P. M. Schumm-Draeger ◽  
K. H. Usadel
Keyword(s):  
Nude Mice ◽  
Graves Disease ◽  
Athymic Nude Mice ◽  
Xenotransplantation Model

scholarly journals Decreasing Ratios of Phosphocreatine to β-ATP Correlates to Progressive Acute Rejection in a Concordant Mouse Heart to Rat Xenotransplantation Model

2001 ◽  
Vol 53 (2) ◽  
pp. 171-175 ◽  
Author(s):  
D. J. Lukes ◽  
B. Madhu ◽  
C. Kjellström ◽  
M. L. Gustavsson ◽  
L. Mjörnstedt ◽  
...  
Keyword(s):  
Acute Rejection ◽  
Mouse Heart ◽  
Xenotransplantation Model

scholarly journals The developmental program of human dendritic cells is operated independently of conventional myeloid and lymphoid pathways

Blood ◽  
2007 ◽  
Vol 110 (10) ◽  
pp. 3591-3660 ◽  
Author(s):  
Fumihiko Ishikawa ◽  
Hiroaki Niiro ◽  
Tadafumi Iino ◽  
Shuro Yoshida ◽  
Noriyuki Saito ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Severe Combined Immunodeficiency ◽  
Molecular Signature ◽  
Newborn Mice ◽  
Nonobese Diabetic ◽  
Genome Wide ◽  
Gene Transcripts ◽  
Plasmacytoid Dcs ◽  
Human Dendritic Cells ◽  
Xenotransplantation Model

Abstract Two distinct dendritic cell (DC) subsets, conventional DCs (cDCs) and plasmacytoid DCs (pDCs), have been shown to develop via either the myeloid or the lymphoid pathway in murine hematopoiesis. Lineage-specific phenotypes or functions of “myeloid” and “lymphoid” DCs, however, still remain elusive. Furthermore, such analysis has been particularly difficult in humans, due to lack of an assay system appropriate for the analysis of human stem and progenitor cell differentiation. Here, using a highly efficient xenotransplantation model, we extensively analyze the origin and the molecular signature of human DCs. Purified human common myeloid progenitors (CMPs) and common lymphoid progenitors (CLPs) were intravenously transplanted into nonobese diabetic–severe combined immunodeficiency (NOD-scid)/IL2rγnull newborn mice. CMPs and CLPs displayed significant expansion in the xenogeneic host, and human cDC and pDC progeny were isolatable. Strikingly, each human DC subset possessed indistinguishable expression patterns of surface phenotype and gene transcripts regardless of their CMP or CLP origin, even at the genome-wide level. Thus, cDC and pDC normally develop after cells have committed to the myeloid or the lymphoid lineage in human hematopoiesis, while their transcriptional signatures are well preserved irrespective of their lineage origin. We propose that human DCs use unique and flexible developmental programs that cannot be categorized into the conventional myeloid or lymphoid pathway.

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