Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

10.3791/61541 ◽  
2020 ◽  
Author(s):  
Bindu Konda ◽  
Apoorva Mulay ◽  
Changfu Yao ◽  
Stephen Beil ◽  
Edo Israely ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Human Lung ◽  
Organoid Culture ◽  
Lung Epithelial ◽  
Epithelial Progenitor Cells

Growth And Differentiation Of Mouse Lung Epithelial Progenitor Cells

2010 ◽  
Author(s):  
Olga Chernaya ◽  
Vasily Shinin ◽  
Oleg Chaga ◽  
Dean E. Schraufnagel ◽  
Richard D. Minshall
Keyword(s):  
Progenitor Cells ◽  
Mouse Lung ◽  
Lung Epithelial ◽  
Epithelial Progenitor Cells

scholarly journals EpCAM+CD73+ mark epithelial progenitor cells in postnatal human lung and are associated with pathogenesis of pulmonary disease including lung adenocarcinoma

2020 ◽  
Vol 319 (5) ◽  
pp. L794-L809
Author(s):  
Limei Wang ◽  
Patrick Dorn ◽  
Cedric Simillion ◽  
Laurène Froment ◽  
Sabina Berezowska ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Progenitor Cells ◽  
Immune Checkpoint ◽  
Human Lung ◽  
Basal Membrane ◽  
Cell Types ◽  
Small Subset ◽  
Lung Lesions ◽  
Immune Checkpoint Molecules ◽  
Epithelial Progenitor Cells

Lung injury in mice induces mobilization of discrete subsets of epithelial progenitor cells to promote new airway and alveolar structures. However, whether similar cell types exist in human lung remains unresolved. Using flow cytometry, we identified a distinct cluster of cells expressing the epithelial cell adhesion molecule (EpCAM), a cell surface marker expressed on epithelial progenitor cells, enriched in the ecto-5′-nucleotidase CD73 in unaffected postnatal human lungs resected from pediatric patients with congenital lung lesions. Within the EpCAM+CD73+ population, a small subset coexpresses integrin β4 and HTII-280. This population remained stable with age. Spatially, EpCAM+CD73+ cells were positioned along the basal membrane of respiratory epithelium and alveolus next to CD73+ cells lacking EpCAM. Expanded EpCAM+CD73+ cells give rise to a pseudostratified epithelium in a two-dimensional air–liquid interface or a clonal three-dimensional organoid assay. Organoids generated under alveolar differentiation conditions were cystic-like and lacked robust alveolar mature cell types. Compared with unaffected postnatal lung, congenital lung lesions were marked by clusters of EpCAM+CD73+ cells in airway and cystic distal lung structures lined by simple epithelium composed of EpCAM+SCGB1A1+ cells and hyperplastic EpCAM+proSPC+ cells. In non-small-cell lung cancer (NSCLC), there was a marked increase in EpCAM+CD73+ tumor cells enriched in inhibitory immune checkpoint molecules CD47 and programmed death-ligand 1 (PD-L1), which was associated with poor survival in lung adenocarcinoma (LUAD). In conclusion, EpCAM+CD73+ cells are rare novel epithelial progenitor cells in the human lung. Importantly, reemergence of CD73 in lung adenocarcinoma enriched in negative immune checkpoint molecules may serve as a novel therapeutic target.

scholarly journals Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells

2020 ◽  
Vol 27 (4) ◽  
pp. 663-678.e8 ◽  
Author(s):  
Antonella F.M. Dost ◽  
Aaron L. Moye ◽  
Marall Vedaie ◽  
Linh M. Tran ◽  
Eileen Fung ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Lung Epithelial ◽  
Epithelial Progenitor Cells

scholarly journals Wnt/β-catenin signaling is critical for regenerative potential of distal lung epithelial progenitor cells in homeostasis and emphysema

Stem Cells ◽  
2020 ◽  
Author(s):  
Yan Hu ◽  
John-Poul Ng-Blichfeldt ◽  
Chiharu Ota ◽  
Chiara Ciminieri ◽  
Wenhua Ren ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Lung Epithelial ◽  
Distal Lung ◽  
Epithelial Progenitor Cells

scholarly journals Behavioral Heterogeneity of Adult Mouse Lung Epithelial Progenitor Cells

2014 ◽  
Vol 23 (22) ◽  
pp. 2744-2757 ◽  
Author(s):  
Olga Chernaya ◽  
Vasily Shinin ◽  
Yuru Liu ◽  
Richard D. Minshall
Keyword(s):  
Progenitor Cells ◽  
Adult Mouse ◽  
Mouse Lung ◽  
Lung Epithelial ◽  
Behavioral Heterogeneity ◽  
Epithelial Progenitor Cells
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