scholarly journals Traction Force Microscopy to Study B Lymphocyte Activation

10.3791/60947 ◽  
2020 ◽  
Author(s):  
Anita Kumari ◽  
Judith Pineau ◽  
Ana-Maria Lennon-Duménil ◽  
Martial Balland ◽  
Paolo Pierobon
Keyword(s):  
B Lymphocyte ◽  
Traction Force ◽  
Lymphocyte Activation ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
B Lymphocyte Activation

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

Effects of Zidovudine on B Lymphocyte Activation

1994 ◽  
Vol 158 (1) ◽  
pp. 140-156 ◽  
Author(s):  
Daniel Widney ◽  
Sigal Yawetz ◽  
Meta van der Meyden ◽  
Steven A. Miles ◽  
Tadamitsu Kishimoto ◽  
...  
Keyword(s):  
B Lymphocyte ◽  
Lymphocyte Activation ◽  
B Lymphocyte Activation

scholarly journals Different Cell Migration Modes: Insights from Traction Force Microscopy and Modeling

2021 ◽  
Vol 120 (3) ◽  
pp. 113a
Author(s):  
Wouter-Jan Rappel ◽  
Elisabeth Ghabache ◽  
Yuansheng Cao ◽  
Yuchuan Miao ◽  
Alexander Groisman ◽  
...  
Keyword(s):  
Cell Migration ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

B lymphocyte activation

1981 ◽  
Vol 2 (9) ◽  
pp. 181-184 ◽  
Author(s):  
Göran Möller
Keyword(s):  
B Lymphocyte ◽  
Lymphocyte Activation ◽  
B Lymphocyte Activation

Suppression of human B lymphocyte activation by ß-endorphin

1990 ◽  
Vol 28 (3) ◽  
pp. 209-217 ◽  
Author(s):  
Edward L. Morgan ◽  
Michael R. McClurg ◽  
Jodee A. Janda
Keyword(s):  
B Lymphocyte ◽  
Lymphocyte Activation ◽  
B Lymphocyte Activation

scholarly journals Epifluorescence-based three-dimensional traction force microscopy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lauren Hazlett ◽  
Alexander K. Landauer ◽  
Mohak Patel ◽  
Hadley A. Witt ◽  
Jin Yang ◽  
...  
Keyword(s):  
Spatial Frequency ◽  
Open Source ◽  
Single Particle ◽  
Three Dimensional ◽  
Traction Force ◽  
High Spatial Frequency ◽  
Epifluorescence Microscopy ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Out Of Plane

Abstract We introduce a novel method to compute three-dimensional (3D) displacements and both in-plane and out-of-plane tractions on nominally planar transparent materials using standard epifluorescence microscopy. Despite the importance of out-of-plane components to fully understanding cell behavior, epifluorescence images are generally not used for 3D traction force microscopy (TFM) experiments due to limitations in spatial resolution and measuring out-of-plane motion. To extend an epifluorescence-based technique to 3D, we employ a topology-based single particle tracking algorithm to reconstruct high spatial-frequency 3D motion fields from densely seeded single-particle layer images. Using an open-source finite element (FE) based solver, we then compute the 3D full-field stress and strain and surface traction fields. We demonstrate this technique by measuring tractions generated by both single human neutrophils and multicellular monolayers of Madin–Darby canine kidney cells, highlighting its acuity in reconstructing both individual and collective cellular tractions. In summary, this represents a new, easily accessible method for calculating fully three-dimensional displacement and 3D surface tractions at high spatial frequency from epifluorescence images. We released and support the complete technique as a free and open-source code package.

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