Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

10.3791/60777 ◽  
2020 ◽  
Author(s):  
Feibo Zheng ◽  
Andy Tully ◽  
Kyle M Koss ◽  
Xiaomin Zhang ◽  
Longhui Qiu ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Hind Limb ◽  
Limb Transplant ◽  
Transplant Model

Combined Co-Stimulatory Blockade and Donor Bone Marrow Cells Induce Robust Immune Tolerance in a Fully MHC-Mismatched Swine Hind Limb Transplant Model

2013 ◽  
Vol 132 ◽  
pp. 141
Author(s):  
Zuhaib Ibrahim ◽  
Angelo A. Leto Barone ◽  
Karim Sarhane ◽  
Joani Christensen ◽  
Georg Furtmueller ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune Tolerance ◽  
Hind Limb ◽  
Bone Marrow Cells ◽  
Donor Bone Marrow ◽  
Limb Transplant ◽  
Transplant Model ◽  
Marrow Cells

scholarly journals PC198. Arterial Calcification Decreases Functional Recovery in a Murine Model of Hind Limb Ischemia

2015 ◽  
Vol 61 (6) ◽  
pp. 171S
Author(s):  
Sara L. Zettervall ◽  
Stephanie D. Monk ◽  
Xue-Lin Wang ◽  
Tonghui Lin ◽  
Raul J. Guzman
Keyword(s):  
Functional Recovery ◽  
Murine Model ◽  
Hind Limb ◽  
Limb Ischemia ◽  
Arterial Calcification ◽  
Hind Limb Ischemia

scholarly journals Imaging the Proangiogenic Effects of Cardiovascular Drugs in a Diabetic Model of Limb Ischemia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
J. L. Goggi ◽  
A. Haslop ◽  
R. Boominathan ◽  
K. Chan ◽  
V. Soh ◽  
...  
Keyword(s):  
Disease Progression ◽  
Functional Recovery ◽  
Pet Imaging ◽  
Murine Model ◽  
Hind Limb ◽  
Immunohistochemical Analysis ◽  
Limb Ischemia ◽  
Vegf Expression ◽  
Vascular Volume ◽  
Hind Limb Ischemia

Purpose. Peripheral artery disease (PAD) causes narrowing of arteries in the limbs, leading to tissue ischemia, gangrene, and eventually limb amputation. The presence of diabetes greatly exacerbates the course of PAD, accounting for the majority of lower limb amputations. Therapeutic strategies focussing on macrovascular repair are less effective in diabetic patients where smaller vessels are affected, and proangiogenic therapies offer a viable adjunct to improve vascularisation in these at risk individuals. The purpose of the current study was to assess the proangiogenic effects of drugs routinely used to treat cardiovascular disease in a diabetic murine model of hind limb ischemia longitudinally using multimodal imaging. Procedures. Diabetic mice underwent surgical intervention to induce hind limb ischemia and were treated with simvastatin, metformin, or a combination orally for 28 days and compared to diabetic and nondiabetic mice. Neovascularisation was assessed using [18F]FtRGD PET imaging, and macrovascular volume was assessed by quantitative time of flight MRI. At each imaging time point, VEGF expression and capillary vessel density were quantified using immunohistochemical analysis, and functional recovery and disease progression were assessed. Results. Combined use of simvastatin and metformin significantly increased neovascularisation above levels measured with either treatment alone. Early angiogenic events were accurately assessed using PET [18F]FtRGD, showing maximal retention in the ischemic hind limb by day 8, which translated to a sustained increase in vascular volume at later time points. Immunohistochemical analysis shows that combined therapy significantly increased VEGF expression and capillary density (CD31+) in a similar time course and also slowed disease progression while simultaneously improving functional foot use. Conclusions. Combined treatment with simvastatin and metformin led to a significant improvement in limb angiogenesis, vascular volume, and sustained functional recovery in a diabetic murine model of HLI. PET imaging with [18F]FtRGD provides a robust method for early detection of these proangiogenic effects preclinically and may be useful for the assessment of proangiogenic therapies used clinically to treat diabetic PAD patients.

Investigation of Antibody-Mediated Rejection in Composite Tissue Allotransplantation in a Rat Limb Transplant Model

2009 ◽  
Vol 41 (2) ◽  
pp. 542-545 ◽  
Author(s):  
J.V. Unadkat ◽  
S. Schneeberger ◽  
C. Goldbach ◽  
M.G. Solari ◽  
K.M. Washington ◽  
...  
Keyword(s):  
Composite Tissue Allotransplantation ◽  
Composite Tissue ◽  
Antibody Mediated Rejection ◽  
Limb Transplant ◽  
Transplant Model

Achieving functional recovery in experimental hind-limb transplantation comparable to replantation

2008 ◽  
Vol 207 (3) ◽  
pp. S107-S108
Author(s):  
Jignesh V. Unadkat ◽  
Mario G. Solari ◽  
Dennis Bourbeau ◽  
Kia McLean ◽  
Paul Afrooz ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Hind Limb

Functional Recovery of Rat Hind-Limb Allografts

2005 ◽  
Vol 21 (07) ◽  
pp. 471-476 ◽  
Author(s):  
You-Xin Song ◽  
Keiichi Muramatsu ◽  
Yoko Kurokawa ◽  
Ryutaro Kuriyama ◽  
Sotetsu Sakamoto ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Hind Limb

scholarly journals Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Quan Liu ◽  
Yong Wang ◽  
Atsunori Nakao ◽  
Wensheng Zhang ◽  
Vijay Gorantla ◽  
...  
Keyword(s):  
Hind Limb ◽  
Third Party ◽  
Donor Strain ◽  
Transplant Tolerance ◽  
Heart Allograft ◽  
Skin Allografts ◽  
Organ Tissue ◽  
Limb Transplant ◽  
End Stage ◽  
Donor Specific Tolerance

Organ/tissue transplantation has become an effective therapy for end-stage diseases. However, immunosuppression after transplantation may cause severe side effects. Donor-specific transplant tolerance was proposed to solve this problem. In this study, we report a novel method for inducing and maintaining heart allograft tolerance rats. First, we induced indefinite vascularized hind-limb allograft survival with a short-term antilymphocyte serum + Cyclosporine A treatment. Peripheral blood chimerism disappeared 6-7 weeks after immunosuppression was withdrawn. Then the recipients accepted secondary donor-strain skin and heart transplantation 200 days following vascularized hind-limb transplantation without any immunosuppression, but rejected third party skin allografts, a status of donor-specific tolerance. The ELISPOT results suggested a mechanism of clone deletion. These findings open new perspectives for the role of vascularized hind-limb transplant in the induction and maintenance of organ transplantation tolerance.

Administration of Particulate Oxygen Generators Improves Skeletal Muscle Contractile Function Following Ischemia-Reperfusion Injury in the Rat Hind Limb

2022 ◽  
Author(s):  
Sarah E. Dyer ◽  
J. David Remer ◽  
Kelsey E. Hannifin ◽  
Aishwarya Hombal ◽  
Joseph C. Wenke ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Functional Recovery ◽  
Hind Limb ◽  
Low Dose ◽  
Contractile Function ◽  
Tissue Injury ◽  
Ischemia Reperfusion ◽  
High Dose ◽  
Extremity Trauma ◽  
The Impact

Extended tourniquet application, often associated with battlefield extremity trauma, can lead to severe ischemia-reperfusion (I/R) injury in skeletal muscle. Particulate oxygen generators (POGs) can be directly injected into tissue to supply oxygen to attenuate the effects of I/R injury in muscle. The goal of this study was to investigate the efficacy of a sodium percarbonate (SPO)-based POG formulation in reducing ischemic damage in a rat hind limb during tourniquet application. Male Lewis rats were anesthetized and underwent tourniquet application for 3 hours, at a pressure of 300 mmHg. Shortly after tourniquet inflation animals received intramuscular injections of either 0.2 mg/mL SPO with catalase (n=6) or 2.0 mg/mL SPO with catalase (n=6) directly into the tibialis anterior (TA) muscle. An additional Tourniquet-Only group (n=12) received no intervention. Functional recovery was monitored using in vivo contractile testing of the hind limb at 1-, 2-, and 4-weeks post-injury. By the 4 week time point, the Low Dose POGs group continued to show improved functional recovery (85% of baseline) compared to the Tourniquet-Only (48%) and High Dose POG (56%) groups. In short, the Low Dose POGs formulation appeared, at least in part, to mitigate the impact of ischemic tissue injury, thus improving contractile function following tourniquet application. Functional improvement correlated with maintenance of larger muscle fiber cross sectional area, and the presence of fewer fibers containing centrally located nuclei. As such, POGs represent a potentially attractive therapeutic solution for addressing I/R injuries associated with extremity trauma.

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