scholarly journals Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

10.3791/60738 ◽  
2020 ◽  
Author(s):  
Luis Gomez-Castillo ◽  
Kurumi Watanabe ◽  
Huayi Jiang ◽  
Shoukai Kang ◽  
Liangcai Gu
Keyword(s):  
Single Domain ◽  
Antibody Library ◽  
Single Domain Antibody ◽  
Protein Dimerization ◽  
Domain Antibody ◽  
Chemically Induced ◽  
Phage Selection ◽  
Selection Of

scholarly journals A VL single-domain antibody library shows a high-propensity to yield non-aggregating binders

2012 ◽  
Vol 25 (6) ◽  
pp. 313-318 ◽  
Author(s):  
G. Hussack ◽  
A. Keklikian ◽  
J. Alsughayyir ◽  
P. Hanifi-Moghaddam ◽  
M. Arbabi-Ghahroudi ◽  
...  
Keyword(s):  
Single Domain ◽  
Antibody Library ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
High Propensity

A disulfide-stabilized human V L single-domain antibody library is a source of soluble and highly thermostable binders

2017 ◽  
Vol 90 ◽  
pp. 190-196 ◽  
Author(s):  
Kevin A. Henry ◽  
Hiba Kandalaft ◽  
Michael J. Lowden ◽  
Martin A. Rossotti ◽  
Henk van Faassen ◽  
...  
Keyword(s):  
Single Domain ◽  
Antibody Library ◽  
Single Domain Antibody ◽  
Domain Antibody

Surface display of a single-domain antibody library on Gram-positive bacteria

2012 ◽  
Vol 70 (6) ◽  
pp. 1081-1093 ◽  
Author(s):  
Filippa Fleetwood ◽  
Nick Devoogdt ◽  
Mireille Pellis ◽  
Ulrich Wernery ◽  
Serge Muyldermans ◽  
...  
Keyword(s):  
Surface Display ◽  
Single Domain ◽  
Antibody Library ◽  
Single Domain Antibody ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Domain Antibody

P-E2 Selection of single domain antibody fragments (nanobodies) from dromedaries immunized with HIV-1 subtype C gp140 SOSIPs

2014 ◽  
Vol 67 ◽  
pp. 94
Author(s):  
Ursula Dietrich ◽  
Kathrin Koch ◽  
Ulrich Wernery ◽  
Kamal Khazanehdari ◽  
Welbeck Danquah ◽  
...  
Keyword(s):  
Antibody Fragments ◽  
Single Domain ◽  
Subtype C ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Hiv 1 ◽  
Selection Of

scholarly journals Selection of a Single Domain Antibody, Specific for an HLA-Bound Epitope of the Mycobacterial Ag85B Antigen

2020 ◽  
Vol 11 ◽  
Author(s):  
Paola A. Ortega ◽  
Mayra Silva-Miranda ◽  
Alfredo Torres-Larios ◽  
Eduardo Campos-Chávez ◽  
Kees C. L. C. M. Franken ◽  
...  
Keyword(s):  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Selection Of

scholarly journals Structural basis for chemically-induced homodimerization of a single domain antibody

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jean Lesne ◽  
Hung-Ju Chang ◽  
Angelique De Visch ◽  
Matteo Paloni ◽  
Philippe Barthe ◽  
...  
Keyword(s):  
Single Domain ◽  
Structural Basis ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Chemically Induced

Anti-idiotypic nanobody as citrinin mimotope from a naive alpaca heavy chain single domain antibody library

2015 ◽  
Vol 407 (18) ◽  
pp. 5333-5341 ◽  
Author(s):  
Yang Xu ◽  
Liang Xiong ◽  
Yanping Li ◽  
Yonghua Xiong ◽  
Zhui Tu ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Single Domain ◽  
Antibody Library ◽  
Single Domain Antibody ◽  
Domain Antibody

Selection of a fully human single domain antibody specific to Helicobacter pylori urease

2019 ◽  
Vol 103 (8) ◽  
pp. 3407-3420 ◽  
Author(s):  
Mehdi Fouladi ◽  
Shamim Sarhadi ◽  
Mohammadreza Tohidkia ◽  
Farnaz Fahimi ◽  
Naser Samadi ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Selection Of

Construction of a Semisynthetic Human VH Single-Domain Antibody Library and Selection of Domain Antibodies against α-Crystalline of Mycobacterium tuberculosis

2015 ◽  
Vol 21 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Nur Hidayah Hairul Bahara ◽  
Siang Tean Chin ◽  
Yee Siew Choong ◽  
Theam Soon Lim
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Selection Process ◽  
Antibody Library ◽  
Affinity Selection ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Variable Heavy ◽  
Vh Domain ◽  
Complementarity Determining Region ◽  
Selection Of

The use of human variable heavy (VH) domain antibodies has been on the rise due to their small scaffold size and simple folding mechanism. A highly diverse library is largely dependent on the diversity introduced within the complementarity-determining region (CDR) cassettes. Here we introduced diversity with the use of a single framework diversifying all three CDRs using tailored codons consisting of degenerate trinucleotides (NNK). The length of the degeneracy in the CDRs was also taken into consideration based on the most frequently occurring length of CDRs and the canonical confirmation for each antibody subfamily. The semisynthetic human VH domain genes were assembled in a single pot using a temperature cascading process. The affinity selection process with Mycobacterium tuberculosis (MTb) α-crystalline was done using a semiautomated process. Enrichment of target-specific clones was observed with successful identification of monoclonal VH domain antibodies for MTb α-crystalline. In short, the semisynthetic library generated was able to select monoclonal VH domain antibodies against full MTb α-crystalline protein with complete semisynthetic CDRs displayed on a single scaffold. The library has the potential to be applied for the isolation of antibodies against other pathogenic proteins.

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