Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

10.3791/60629 ◽  
2020 ◽  
Author(s):  
Immacolata Belviso ◽  
Anna Maria Sacco ◽  
Veronica Romano ◽  
Fabrizio Schonauer ◽  
Daria Nurzynska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Abdominal Skin ◽  
Adult Human ◽  
Induced Pluripotent

scholarly journals Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells

Stem Cells ◽  
2015 ◽  
Vol 34 (1) ◽  
pp. 246-251 ◽  
Author(s):  
Tomas Barta ◽  
Lucie Peskova ◽  
Joseph Collin ◽  
David Montaner ◽  
Irina Neganova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Induced Pluripotent

Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells

2021 ◽  
Author(s):  
Immacolata Belviso ◽  
Franca Di Meglio ◽  
Veronica Romano ◽  
Stefania Montagnani ◽  
Clotilde Castaldo
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Induced Pluripotent

scholarly journals Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors

2010 ◽  
Vol 6 (1) ◽  
pp. 78-88 ◽  
Author(s):  
Kazim H Narsinh ◽  
Fangjun Jia ◽  
Robert C Robbins ◽  
Mark A Kay ◽  
Michael T Longaker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Adult Human ◽  
Induced Pluripotent ◽  
Dna Vectors ◽  
Minicircle Dna

scholarly journals Generation of induced pluripotent stem cells from human blood

Blood ◽  
2009 ◽  
Vol 113 (22) ◽  
pp. 5476-5479 ◽  
Author(s):  
Yuin-Han Loh ◽  
Suneet Agarwal ◽  
In-Hyun Park ◽  
Achia Urbach ◽  
Hongguang Huo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Induced Pluripotent Stem Cells ◽  
Human Blood ◽  
Pluripotent Stem Cells ◽  
Methylation Status ◽  
Embryonic Stem ◽  
Dermal Fibroblasts ◽  
Patient Specific ◽  
Induced Pluripotent

Human dermal fibroblasts obtained by skin biopsy can be reprogrammed directly to pluripotency by the ectopic expression of defined transcription factors. Here, we describe the derivation of induced pluripotent stem cells from CD34+ mobilized human peripheral blood cells using retroviral transduction of OCT4/SOX2/KLF4/MYC. Blood-derived human induced pluripotent stem cells are indistinguishable from human embryonic stem cells with respect to morphology, expression of surface antigens, and pluripotency-associated transcription factors, DNA methylation status at pluripotent cell-specific genes, and the capacity to differentiate in vitro and in teratomas. The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.

scholarly journals Generation of human induced pluripotent stem cells from dermal fibroblasts

2008 ◽  
Vol 105 (8) ◽  
pp. 2883-2888 ◽  
Author(s):  
W. E. Lowry ◽  
L. Richter ◽  
R. Yachechko ◽  
A. D. Pyle ◽  
J. Tchieu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Dermal Fibroblasts ◽  
Induced Pluripotent
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