scholarly journals Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

10.3791/60447 ◽  
2019 ◽  
Author(s):  
Klemens Weisleitner ◽  
Lars Hunger ◽  
Christoph Kohstall ◽  
Albert Frisch ◽  
Michael C. Storrie-Lombardi ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Laser Induced Fluorescence ◽  
In Situ Measurements ◽  
Non Invasive

L.I.F.E.: laser induced fluorescence emission, a non-invasive tool to detect photosynthetic pigments in glacial ecosystems

2011 ◽  
Author(s):  
Markus Tilg ◽  
Michael Storrie-Lombardi ◽  
Christoph Kohstall ◽  
Andreas Trenkwalder ◽  
Roland Psenner ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Fluorescence Emission ◽  
Laser Induced Fluorescence ◽  
Non Invasive

A Laser Induced Fluorescence-Based Instrument for In-Situ Measurements of Atmospheric Formaldehyde

2009 ◽  
Vol 43 (3) ◽  
pp. 790-795 ◽  
Author(s):  
John R. Hottle ◽  
Andrew J. Huisman ◽  
Joshua P. DiGangi ◽  
Aster Kammrath ◽  
Melissa M. Galloway ◽  
...  
Keyword(s):  
Laser Induced Fluorescence ◽  
In Situ Measurements

scholarly journals Improvements to a laser-induced fluorescence instrument for measuring SO<sub>2</sub>: impact on accuracy and precision

2020 ◽  
Author(s):  
Pamela S. Rickly ◽  
Lu Xu ◽  
John D. Crounse ◽  
Paul O. Wennberg ◽  
Andrew W. Rollins
Keyword(s):  
Limit Of Detection ◽  
Fluorescence Emission ◽  
Laser Induced Fluorescence ◽  
Fluorescence Signal ◽  
Fluorescence Emission Spectrum ◽  
Signal Ratio ◽  
Accuracy And Precision ◽  
Aromatic Species

Abstract. This work describes improvements made to the in-situ laser induced fluorescence SO2 instrument as originally described in Rollins et al. (2016). We report measurements of the SO2 fluorescence emission spectrum. These measurements allow for the determination of the most appropriate bandpass filters to optimize the fluorescence signal while reducing the instrumental background. Because many aromatic species fluoresce in the same spectral region as SO2, fluorescence spectra were also measured for naphthalene and anisole to determine if ambient SO2 measurements may be biased in the presence of such species. In addition, the 216.9 nm laser linewidth was decreased in order to increase the online/offline signal ratio which in-turn increases the precision of the measurement. The effects of these improvements on the instrumental sensitivity were determined by analyzing the signal and background of the instrument using varying optical bandpass filter ranges and cell pressures and calculating the resulting limit of detection. As a result, we report an improvement to the instrumental sensitivity by as much as 50 %.

scholarly journals Application of the TDR measuring technique for in-situ measurements using surface probes

2011 ◽  
Vol 8 (1) ◽  
pp. 097-106
Author(s):  
Zbigniew Suchorab ◽  
Zbyšek Pavlík ◽  
Robert Černý
Keyword(s):  
Time Domain ◽  
Time Domain Reflectometry ◽  
In Situ Measurements ◽  
Measuring Technique ◽  
Ceramic Brick ◽  
Non Invasive

The article presents the possibility of in-situ measurements of building barriers moisture using the TDR technique (Time Domain Reflectometry). To minimize the previous disadvantages of the described method – invasive character, several prototypes of the surface probes were manufactured. With such a sensor solution it was possible to conduct the non-invasive measurements using the full TDR method potential – quick measurements and no sensitivity of salinity influence. The measurements were conducted on the brickworks made of red ceramic brick at the old building located on 1st of May Street in Lublin.

scholarly journals Laser-induced fluorescence emission (LIFE) from Lake Fryxell (Antarctica) cryoconites

2010 ◽  
Vol 51 (56) ◽  
pp. 145-152 ◽  
Author(s):  
Birgit Sattler ◽  
Michael C. Storrie-Lombardi ◽  
Christine M. Foreman ◽  
Markus Tilg ◽  
Roland Psenner
Keyword(s):  
Ice Core ◽  
Fluorescence Emission ◽  
Digital Camera ◽  
Laser Induced Fluorescence ◽  
Green Laser ◽  
Fluorescence Response ◽  
Total Pigment ◽  
Reflection And Refraction ◽  
Lake Fryxell

AbstractLaser-induced fluorescence emission (LIFE) images were obtained in situ from a 27 cm long ice core at Lake Fryxell, Antarctica. The excitation was accomplished with a simple 532 nm green laser pen light, and the fluorescence images were captured with a small compact digital camera. The targets for the experiment were mm-scale cryoconite assemblages found in the ice covers of this perennially frozen Antarctic lake. The fluorescence response originates from photo-pigments in cyanobacteria-dominated cryoconite assemblages with phycoerythrin (PE) exhibiting the optimal target cross section. This inexpensive, low-mass, low-energy method avoids manipulation of the in situ habitat and individual target organisms and does not disturb the microbial community or the surrounding ice matrix. We establish the correlation between fluorescence intensity and PE concentration. We show that cryoconite fluorescence response does not appear to decrease with depth in the ice cover, in agreement with similar findings at Lake Untersee, a perennially ice-covered lake in Dronning Maud Land, Antarctica. Optical reflection and refraction events at the air/ice interface can complicate quantitative estimates of total pigment concentrations. Laser targeting of a single mm-scale cryoconite can result in multiple neighboring excitation events secondary to reflection and refraction phenomena in the multiple air/ice interface of the bubbles surrounding the primary target.

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