Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

10.3791/60347 ◽  
2019 ◽  
Author(s):  
Lu Zhou ◽  
Qingyun Li
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Mouse Brain ◽  
Adult Mouse ◽  
Adult Mouse Brain ◽  
Brain Hemisphere ◽  
Single Cell Rna Sequencing

scholarly journals Expression of the Na+/K+-ATPase Subunits in Adult Mouse Brain Analyzed by Single-Cell RNA-Seq Profiling

2019 ◽  
Vol 116 (3) ◽  
pp. 129a
Author(s):  
Song Jiao ◽  
Cristina Moreno Vadillo ◽  
Miguel Holmgren
Keyword(s):  
Single Cell ◽  
Mouse Brain ◽  
Adult Mouse ◽  
Rna Seq ◽  
Adult Mouse Brain ◽  
Atpase Subunits

scholarly journals Single-Cell RNA Sequencing of the Adult Mouse Kidney: From Molecular Cataloging of Cell Types to Disease-Associated Predictions

2019 ◽  
Vol 73 (1) ◽  
pp. 140-142 ◽  
Author(s):  
Nils O. Lindström ◽  
Guilherme De Sena Brandine ◽  
Andrew Ransick ◽  
Andrew P. McMahon
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Adult Mouse ◽  
Cell Types ◽  
Mouse Kidney ◽  
Single Cell Rna Sequencing

scholarly journals Single-cell RNA sequencing identifies senescent cerebromicrovascular endothelial cells in the aged mouse brain

GeroScience ◽  
2020 ◽  
Vol 42 (2) ◽  
pp. 429-444 ◽  
Author(s):  
Tamas Kiss ◽  
Ádám Nyúl-Tóth ◽  
Priya Balasubramanian ◽  
Stefano Tarantini ◽  
Chetan Ahire ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Single Cell ◽  
Rna Sequencing ◽  
Mouse Brain ◽  
Aged Mouse ◽  
Single Cell Rna Sequencing

scholarly journals Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing

2021 ◽  
Vol 2 (3) ◽  
pp. 100715
Author(s):  
Akari Yamazaki ◽  
Francis Shue ◽  
Yu Yamazaki ◽  
Yuka A. Martens ◽  
Guojun Bu ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Mouse Brain ◽  
Cell Suspensions ◽  
Vascular Cells ◽  
Single Cell Rna Sequencing

scholarly journals Single-cell RNA sequencing of adult mouse testes

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Soeren Lukassen ◽  
Elisabeth Bosch ◽  
Arif B. Ekici ◽  
Andreas Winterpacht
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Adult Mouse ◽  
Single Cell Rna Sequencing

scholarly journals A Single-Cell Atlas of Cell Types, States, and Other Transcriptional Patterns from Nine Regions of the Adult Mouse Brain

2018 ◽  
Author(s):  
Arpiar Saunders ◽  
Evan Macosko ◽  
Alec Wysoker ◽  
Melissa Goldman ◽  
Fenna Krienen ◽  
...  
Keyword(s):  
Basal Ganglia ◽  
Substantia Nigra ◽  
Single Cell ◽  
Globus Pallidus ◽  
Mouse Brain ◽  
Adult Mouse ◽  
Adult Mouse Brain ◽  
Neuronal Populations ◽  
The Brain ◽  
Globus Pallidus Externus

The mammalian brain is composed of diverse, specialized cell populations, few of which we fully understand. To more systematically ascertain and learn from cellular specializations in the brain, we used Drop-seq to perform single-cell RNA sequencing of 690,000 cells sampled from nine regions of the adult mouse brain: frontal and posterior cortex (156,000 and 99,000 cells, respectively), hippocampus (113,000), thalamus (89,000), cerebellum (26,000), and all of the basal ganglia – the striatum (77,000), globus pallidus externus/nucleus basalis (66,000), entopeduncular/subthalamic nuclei (19,000), and the substantia nigra/ventral tegmental area (44,000). We developed computational approaches to distinguish biological from technical signals in single-cell data, then identified 565 transcriptionally distinct groups of cells, which we annotate and present through interactive online software we developed for visualizing and re-analyzing these data (DropViz). Comparison of cell classes and types across regions revealed features of brain organization. These included a neuronal gene-expression module for synthesizing axonal and presynaptic components; widely shared patterns in the combinatorial co-deployment of voltage-gated ion channels by diverse neuronal populations; functional distinctions among cells of the brain vasculature; and specialization of glutamatergic neurons across cortical regions to a degree not observed in other neuronal or non-neuronal populations. We describe systematic neuronal classifications for two complex, understudied regions of the basal ganglia, the globus pallidus externus and substantia nigra reticulata. In the striatum, where neuron types have been intensely researched, our data reveal a previously undescribed population of striatal spiny projection neurons (SPNs) comprising 4% of SPNs. The adult mouse brain cell atlas can serve as a reference for analyses of development, disease, and evolution.

scholarly journals Single-cell RNA sequencing reveals midbrain dopamine neuron diversity emerging during mouse brain development

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Katarína Tiklová ◽  
Åsa K. Björklund ◽  
Laura Lahti ◽  
Alessandro Fiorenzano ◽  
Sara Nolbrant ◽  
...  
Keyword(s):  
Brain Development ◽  
Single Cell ◽  
Rna Sequencing ◽  
Mouse Brain ◽  
Dopamine Neuron ◽  
Single Cell Rna Sequencing ◽  
Neuron Diversity ◽  
Midbrain Dopamine ◽  
Mouse Brain Development

scholarly journals Single-cell RNA sequencing of mouse brain and lung vascular and vessel-associated cell types

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Liqun He ◽  
Michael Vanlandewijck ◽  
Maarja Andaloussi Mäe ◽  
Johanna Andrae ◽  
Koji Ando ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Mouse Brain ◽  
Cell Types ◽  
Single Cell Rna Sequencing

scholarly journals Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing

2021 ◽  
Vol 2 (2) ◽  
pp. 100590
Author(s):  
Lu Liu ◽  
Simon Besson-Girard ◽  
Hao Ji ◽  
Katrin Gehring ◽  
Buket Bulut ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Single Cell Rna Sequencing
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