scholarly journals Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

10.3791/60130 ◽  
2019 ◽  
Author(s):  
Chi-Keung Cheng ◽  
Terry H.Y. Wong ◽  
Yuk-Lin Yung ◽  
Nelson C.N. Chan ◽  
Margaret H.L. Ng
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Acute Myeloid Leukemia Cells ◽  
Acute Myeloid

scholarly journals Icariside II Induces Apoptosis in U937 Acute Myeloid Leukemia Cells: Role of Inactivation of STAT3-Related Signaling

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e28706 ◽  
Author(s):  
Sang-Hun Kang ◽  
Soo-Jin Jeong ◽  
Sun-Hee Kim ◽  
Ji-Hyun Kim ◽  
Ji Hoon Jung ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Acute Myeloid Leukemia Cells ◽  
Icariside Ii ◽  
Acute Myeloid

scholarly journals Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 505
Author(s):  
Minjing Li ◽  
Shiyu Hao ◽  
Chunling Li ◽  
Huimin Xiao ◽  
Liyuan Sun ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Growth And Survival ◽  
Elevated Expression ◽  
Acute Myeloid Leukemia Cells ◽  
Poor Outcomes ◽  
Induced Apoptosis ◽  
Acute Myeloid

Current strategies are not especially successful in the treatment of acute myeloid leukemia (AML). The identification and characterization of oncogenes crucial to the survival and growth of leukemia cells will provide potential targets for the exploitation of novel therapies. Herein, we report that the elevated expression of SH3 domain-binding protein 5 (SH3BP5) significantly correlates with poor outcomes of AML patients. To test whether SH3BP5 contributes to the growth and survival of AML cells, we use the shRNA-encoding lentivirus system to achieve the knockdown of SH3BP5 expression in human AML cell lines U937, THP-1, Kasumi-1, and MV4-11. Functionally, the knockdown of SH3BP5 expression markedly inhibits the cell viability and induced apoptosis of these leukemia cells. Mechanistically, western blot analysis indicates that the knockdown of SH3BP5 expression decreases the phosphorylation of JNK and BAD. Moreover, the JNK agonist anisomycin rescues the growth inhibition phenotype of SH3BP5 deficiency in THP-1 cells. Moreover, the expression of SH3BP5 positively correlates with CD25 and CD123 levels. Finally, our study highlights the crucial role of SH3BP5 in promoting the survival of AML cells, and its suppression may be a potential therapeutic strategy for treating human AML.

scholarly journals Hyper-SUMOylation of ERG Is Essential for the Progression of Acute Myeloid Leukemia

2021 ◽  
Vol 8 ◽  
Author(s):  
Xu Chen ◽  
Yuanyuan Qin ◽  
Zhenzhen Zhang ◽  
Zhengcao Xing ◽  
Qiqi Wang ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Crucial Role ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Hematopoietic Tissue ◽  
Acute Myeloid Leukemia Cells ◽  
Hematopoietic Precursor ◽  
Ets Transcription Factor ◽  
Acute Myeloid

Leukemia is a malignant disease of hematopoietic tissue characterized by the differentiation arrest and malignant proliferation of immature hematopoietic precursor cells in bone marrow. ERG (ETS-related gene) is an important member of the E26 transformation-specific (ETS) transcription factor family that plays a crucial role in physiological and pathological processes. However, the role of ERG and its modification in leukemia remains underexplored. In the present study, we stably knocked down or overexpressed ERG in leukemia cells and observed that ERG significantly promotes the proliferation and inhibits the differentiation of AML (acute myeloid leukemia) cells. Further experiments showed that ERG was primarily modified by SUMO2, which was deconjugated by SENP2. PML promotes the SUMOylation of ERG, enhancing its stability. Arsenic trioxide decreased the expression level of ERG, further promoting cell differentiation. Furthermore, the mutation of SUMO sites in ERG inhibited its ability to promote the proliferation and inhibit the differentiation of leukemia cells. Our results demonstrated the crucial role of ERG SUMOylation in the development of AML, providing powerful targeted therapeutic strategies for the clinical treatment of AML.

scholarly journals Pro-survival role of p62 during granulocytic differentiation of acute myeloid leukemia cells

2014 ◽  
Vol 1 (4) ◽  
pp. e970066 ◽  
Author(s):  
Evelyne Ségal-Bendirdjian ◽  
Mario P Tschan ◽  
Josy Reiffers ◽  
Mojgan Djavaheri-Mergny
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Granulocytic Differentiation ◽  
Acute Myeloid Leukemia Cells ◽  
Acute Myeloid

Role of selenite in differentiation of acute myeloid leukemia cells

2012 ◽  
Vol 53 ◽  
pp. S117-S118
Author(s):  
S. Misra⁎ ◽  
M. Wallenberg ◽  
A. Barsham ◽  
M. Björnstedt ◽  
A. Fernandes
Keyword(s):  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Acute Myeloid Leukemia Cells ◽  
Acute Myeloid
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