scholarly journals Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

10.3791/59905 ◽  
2019 ◽  
Author(s):  
Robert S. Brzozowski ◽  
Maria L. White ◽  
Prahathees J. Eswara
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Morphology ◽  
Protein Localization ◽  
Live Cell ◽  
Subcellular Protein Localization ◽  
Morphology Changes

Initial Attachment of MC3T3-E1 Cells on Nano-HAp Surfaces

2007 ◽  
Vol 361-363 ◽  
pp. 1115-1118
Author(s):  
Un Hye Kwon ◽  
Jung Suk Han ◽  
In Young Ryu ◽  
Dae Joon Kim
Keyword(s):  
Surface Roughness ◽  
Cell Adhesion ◽  
Fluorescence Microscopy ◽  
Cell Morphology ◽  
Confocal Laser ◽  
Initial Attachment ◽  
Zirconia Ceramics ◽  
Initial Cell ◽  
Cell Adhesion Proteins ◽  
Initial Cell Adhesion

The initial osteoblast like cell response to bioactive nano-sized hydroxyapatite (HAp) and bioinert zirconia was evaluated with the cell morphology by SEM and cell adhesion proteins by fluorescence microscopy. Surface roughness also measured by a confocal laser microscopy. The surface roughness and topography was almost identical among specimens. The nano-sized HAp specimens showed better initial cell adhesion and activity than bioinert zirconia ceramics.

scholarly journals Full-field supercritical angle fluorescence microscopy for live cell imaging

2011 ◽  
Vol 36 (16) ◽  
pp. 3051 ◽  
Author(s):  
Thomas Barroca ◽  
Karla Balaa ◽  
Julie Delahaye ◽  
Sandrine Lévêque-Fort ◽  
Emmanuel Fort
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Full Field

TheArabidopsis Localizome: Subcellular Protein Localization and Interactions inARABIDOPSIS

2008 ◽  
pp. 61-81
Author(s):  
Georgios Kitsios ◽  
Nicolas Tsesmetzis ◽  
Max Bush ◽  
John H. Doonan
Keyword(s):  
Protein Localization ◽  
Subcellular Protein Localization

Dictyostelium Discoideum: Live Cell Imaging in Changing Perspective

2020 ◽  
pp. 126-145
Keyword(s):  
Dictyostelium Discoideum ◽  
Protein Localization ◽  
Red Light ◽  
Live Cell ◽  
Cell Organelle ◽  
Light Spectrum ◽  
Localization Study ◽  
Cell Localization ◽  
Uv Visible ◽  
Fluorescent Tags

The advent of advanced microscopes; during microscope evolution from simple microscopes to confocal and live cell microscope; having digital imaging facility revolutionized our view for the living cells. In the protein localization study, fluorescent proteins are tagged at amino or carboxyl (preferably) terminal of desired protein for live cell study. These live cell studies improved our understanding of protein dynamics and understanding its role in biological regulation. The mutational variants of fluorescent tags (GFP, RFP); can be used with different protein; which will efficiently use UV-Visible to Far Red light spectrum; without overlapping of excitation and emission spectrum. Further, various cell organelle (Lysosome, Golgi bodies, Endoplasmic Reticulum, Mitochondria, Nucleus) trackers; improved our live cell localization studies in the wide non-overlapping UV-Visible spectrum.This chapter gives an overview for live cell protein localization study in mitotically active, unicellular stage of Dictyostelium discoideum. This evolutionary cutting edge organism had both unicellular as well as multicellular stages during its life cycle. This chapter will provide the design of fusion of fluorescent tag to the specific gene and its live cell localization. Further, it will cover; transformation of the unicellular organism; drug based selection; sample preparation with nuclear, mitochondrial localization markers (trackers) and live cell localization study on live cell-confocal microscope setup. It will also have a glimpse of the design of fusion protein with an aspect of advantage and disadvantages.

scholarly journals Live cell fluorescence microscopy to study microbial pathogenesis

2009 ◽  
Vol 11 (4) ◽  
pp. 540-550 ◽  
Author(s):  
Adam D. Hoppe ◽  
Stephanie Seveau ◽  
Joel A. Swanson
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell ◽  
Microbial Pathogenesis
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