Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

10.3791/59491 ◽  
2019 ◽  
Author(s):  
Alexander Hamilton ◽  
Elisa Vergari ◽  
Caroline Miranda ◽  
Andrei I. Tarasov
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Calcium Dynamics ◽  
Pancreatic Islet Cells ◽  
Mouse Pancreatic Islet

Carbamoylcholine regulation of polyphosphoinositide synthesis and hydrolysis in cultured, dispersed, digitonin-permeabilized mouse pancreatic islet cells

1997 ◽  
Vol 136 (5) ◽  
pp. 539-545 ◽  
Author(s):  
Andrew M Kardasz ◽  
Peter Thams ◽  
Kirsten Capito ◽  
Carl J Hedeskov
Keyword(s):  
Pancreatic Islet ◽  
Kinase Activity ◽  
Inositol Phosphates ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Mouse Pancreatic Islet ◽  
Phosphatidylinositol 4 ◽  
Stimulation Of

Abstract Continuing formation of inositol phosphates during stimulation of pancreatic β-cells by hormones and neurotransmitters requires the continued synthesis of the polyphosphoinositides phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5 bisphosphate (PIP2) from phosphatidylinositol (PI). In the present study we have investigated how this pathway and the activity of phosphoinositide-specific phospholipase C (PI-PLC) are regulated by carbamoylcholine (CCh), Ca2+, the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), GTPγS and NaF in 44-h [3H]inositol-labelled, dispersed and digitonin-permeabilized mouse pancreatic islet cells. CCh stimulated not only PI-PLC (G-protein-mediated) but also, by an as yet unknown mechanism, significantly enhanced PI 4-kinase activity, estimated as the PIP:PI ratio, by 100%, and further increased the flux from PI to PIP and PIP2. GTPγS and NaF mimicked the effects of CCh on PI-PLC but had no effect on the levels of PIP and PIP2. TPA raised the PIP:PI ratio by 75%. In addition TPA counteracted the CCh stimulation of PI-PLC. There was no effect of 10−6 mol/l Ca2+ on the levels of PIP and PIP2. Experiments with quinacrine and adenosine confirmed that PI-PLC and PI 4-kinase could be regulated independently of each other. In conclusion, these data point to differential regulation of polyphosphoinositide synthesis and breakdown. European Journal of Endocrinology 136 539–545

Cytotoxic activation of complement by mouse pancreatic islet cells

Diabetes ◽  
1980 ◽  
Vol 29 (8) ◽  
pp. 636-642 ◽  
Author(s):  
L. A. Idahl ◽  
J. Sehlin ◽  
I. B. Taljedal ◽  
L. E. Thornell
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Mouse Pancreatic Islet

Cytotoxic Activation of Complement by Mouse Pancreatic Islet Cells

Diabetes ◽  
1980 ◽  
Vol 29 (8) ◽  
pp. 636-642 ◽  
Author(s):  
L.-A. Idahl ◽  
J. Sehlin ◽  
I.-B. Taljedal ◽  
L.-E. Thornell
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Mouse Pancreatic Islet

scholarly journals Notch signalling suppresses apoptosis in adult human and mouse pancreatic islet cells

Diabetologia ◽  
2007 ◽  
Vol 50 (12) ◽  
pp. 2504-2515 ◽  
Author(s):  
V. Dror ◽  
V. Nguyen ◽  
P. Walia ◽  
T. B. Kalynyak ◽  
J. A. Hill ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Notch Signalling ◽  
Pancreatic Islet Cells ◽  
Adult Human ◽  
Mouse Pancreatic Islet ◽  
Human And Mouse

scholarly journals Influence of cell number on the characteristics and synchrony of Ca2+oscillations in clusters of mouse pancreatic islet cells

1999 ◽  
Vol 520 (3) ◽  
pp. 839-849 ◽  
Author(s):  
Françoise C. Jonkers ◽  
Jean-Christophe Jonas ◽  
Patrick Gilon ◽  
Jean-Claude Henquin
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Cell Number ◽  
Pancreatic Islet Cells ◽  
Mouse Pancreatic Islet
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