Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

10.3791/59437 ◽  
2019 ◽  
Author(s):  
Melissa Bredow ◽  
Irina Sementchoukova ◽  
Kristen Siegel ◽  
Jacqueline Monaghan
Keyword(s):  
Arabidopsis Thaliana ◽  
Growth Inhibition ◽  
Seedling Growth ◽  
Oxidative Burst ◽  
Seedling Growth Inhibition

Two Arabidopsis thaliana dihydrodipicolinate synthases, DHDPS1 and DHDPS2, are unequally redundant

2012 ◽  
Vol 39 (12) ◽  
pp. 1058 ◽  
Author(s):  
Susan Jones-Held ◽  
Luciana Pimenta Ambrozevicius ◽  
Michael Campbell ◽  
Bradley Drumheller ◽  
Emily Harrington ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Arabidopsis Thaliana ◽  
Growth Inhibition ◽  
Segregation Analysis ◽  
Narrow Range ◽  
Insertion Mutant ◽  
Biosynthesis Pathway ◽  
Wild Type ◽  
Exogenous Feeding ◽  
Growth Inhibitory

In Arabidopsis thalinana (L.) Heynh., DHDPS1 and DHDPS2 encode orthologous dihydrodipicolinate synthases (DHDPS), the first enzyme of the lysine (Lys) biosynthesis pathway. A TDNA insertion mutant of dhdps2 was previously reported to be viable and to accumulate free threonine (Thr). Analysis of additional TDNA insertion lines showed that dhdps1 and dhdps2 mutants are both viable and that whereas dhdps2 mutants accumulate Thr, dhdps1 plants do not. Thr-accumulation was complemented by heterologous expression of Escherichia coli DapA, indicating that the phenotype is due to reduced DHDPS activity in dhdps2. DHDPS1 contributes ~30% towards the total DHDPS activity in leaves of young plants and DHDPS2 contributes 70%; therefore, the threshold of activity resulting in Thr accumulation lies within this narrow range. dhdps1–dhdps2 double mutants could not be isolated, even after exogenous feeding with Lys. Segregation analysis indicated that gametes lacking functional DHDPS genes are defective, as are embryos. Plants carrying only a single DHDPS2 gene do not accumulate Thr, but they show a gametophytic defect that is partially rescued by Lys application. Despite the accumulation of Thr, dhdps2 seedlings are no more sensitive than wild-type plants to growth inhibition by Lys or the Lys precursor diaminopimelate. They also are not rescued by methionine at growth-inhibitory Lys concentrations. Exogenous application of Lys and methionine to dhdps2 mutants did not reduce the accumulation of Thr.

An RRM-containing mei2 -like MCT1 plays a negative role in the seed germination and seedling growth of Arabidopsis thaliana in the presence of ABA

2016 ◽  
Vol 109 ◽  
pp. 273-279 ◽  
Author(s):  
Lili Gu ◽  
Hyun Ju Jung ◽  
Kyung Jin Kwak ◽  
Sy Nguyen Dinh ◽  
Yeon-Ok Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Seedling Growth ◽  
Negative Role
Sign in / Sign up

Export Citation Format

Share Document