Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

10.3791/59213 ◽  
2019 ◽  
Author(s):  
Elizabeth C. Bondy ◽  
Martha S. Hunter
Keyword(s):  
Bemisia Tabaci ◽  
Fertilization Rate ◽  
Cytogenetic Technique

CFTR protein expression in healthy men spermatozoa is not correlated with fertilization rate of ova

2010 ◽  
Vol 34 (8) ◽  
pp. S12-S12
Author(s):  
Hong‑Ge Li ◽  
Chen Min Xu ◽  
Kun Li ◽  
Ya Ni ◽  
Wen‑Ying Chen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Fertilization Rate ◽  
Healthy Men ◽  
Cftr Protein

Control of the Bemisia tabaci/Tomato yellow leaf curl virus complex on protected tomato crops in Algarve (Portugal)*

EPPO Bulletin ◽  
2002 ◽  
Vol 32 (1) ◽  
pp. 31-35
Author(s):  
A. F. Arsenio ◽  
E. Neto ◽  
N. Ramos ◽  
S. Mangerico ◽  
E. Fortunato ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Virus Complex ◽  
Leaf Curl Virus ◽  
Leaf Curl

Improvement of Ctenopharyngodon idella reproduction technology under the conditions of water fish fishery

2020 ◽  
pp. 58-65
Author(s):  
I. Nikishkin ◽  
L. Zakonnova
Keyword(s):  
Energy Production ◽  
Fertilization Rate ◽  
Ctenopharyngodon Idella ◽  
Hormonal Stimulation ◽  
Hydrochemical Regime ◽  
Early Maturation ◽  
Planting Material ◽  
Products Analysis ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Herbivorous Species

The most rational way to reduce the eutrophic load on reservoirs-coolers of energy enterprises, in which the technological processes of energy production and growing fish products are not disrupted, is the introduction of fish-reclaimers into the reservoir. In this regard, full-system fisheries of this type include the production of fish-planting material of herbivorous species, in particular grass carp Ctenopharyngodon idella in their work plan. In the production conditions of Belovsky Fisheries LLC, a technology for growing planting material and commercial products of the white Amur has been developed, which has fully justified itself. At the same time, certain difficulties are associated with obtaining high-quality sexual products of herbivorous fish. Due to the technological features and hydrochemical regime of the reservoir, the production of plant-eating fish planting material is carried out with a high risk of over-aging of sexual products. Analysis of the results of spawning campaigns revealed a number of problems, including: zero percent of fertilization, early maturation and release of part of the eggs by females after a permissive injection, and gonad thrombosis. To solve the problem of low fertilization rate, special attention should be paid to sufficient oxygen concentration in water during pre-spawning operations with producers of Cupid. The problem of over-aging of sexual products and gonad thrombosis is proposed to be solved by using a flexible scheme of hormonal stimulation of producers.

scholarly journals Determination of seasonal activity of the sweetpotato whitefly (Homoptera: Aleyrodidae) and leafhoppers (Homoptera: Cicadellidae) by plastic cup traps on the Çukurova Plain, Turkey.

2010 ◽  
Vol 41 (No. 1) ◽  
pp. 8-13 ◽  
Author(s):  
K. Karut ◽  
C. Chu C ◽  
T.J. Henneberry ◽  
C. Kazak
Keyword(s):  
Bemisia Tabaci ◽  
Seasonal Activity ◽  
Sweetpotato Whitefly ◽  
Daily Minimum ◽  
Trap Catches

The flight activities of adult sweetpotato whitefly, Bemisia tabaci, and leafhoppers were monitored by plastic cup traps at Boğalı, Taşçı, Hacıali, Doğankent and Balcalı in the Çukurova Plain, Turkey, in 2001 and 2003. Activity of B. tabaci, expressed as numbers of adults caught in traps, was low from May to early July in both years. Numbers of B. tabaci caught at Tasçı were higher than at Boğalı from 10 July and 21 August in 2001. Its numbers were also higher at Doğankent than at Hacıali and Balcalı during August of 2003. The numbers of leafhopper adults caught fluctuated greatly in both years. Numbers remained low until late June, followed by gradual increases in July and August at Boğalı and Taşçı in 2001. In contrast, numbers of adults caught were higher at Hacıali, Doğankent and Balcalı from May through July in 2003, followed by lower catches during the remainder of the season. Daily minimum temperatures in July and August were positively correlated with higher trap catches of both B. tabaci and leafhoppers.

Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽  
2020 ◽  
Vol 28 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Jie Zhang ◽  
De-Ling Kong ◽  
Bin Xiao ◽  
Hong-Jie Yuan ◽  
Qiao-Qiao Kong ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Restraint Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Testicular Cells ◽  
Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

scholarly journals Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Reproduction ◽  
2013 ◽  
Vol 146 (2) ◽  
pp. 119-133 ◽  
Author(s):  
Barbara Ambruosi ◽  
Gianluca Accogli ◽  
Cécile Douet ◽  
Sylvie Canepa ◽  
Géraldine Pascal ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Western Blot ◽  
Immunohistochemical Analysis ◽  
Fertilization Rate ◽  
Malignant Brain Tumor ◽  
Estrus Cycle ◽  
Immunofluorescence Analysis ◽  
Oviductal Fluid ◽  
Porcine Spermatozoa

Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus–oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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