Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

10.3791/58830 ◽  
2019 ◽  
Author(s):  
Barry W. Neun ◽  
Marina A. Dobrovolskaia
Keyword(s):  
Limulus Amoebocyte Lysate

scholarly journals Role of insulin during the development of oligofructose (OF)-induced equine laminitis

2015 ◽  
Vol 59 (2) ◽  
pp. 303-309
Author(s):  
Renli Jiang ◽  
Li Gao ◽  
Guanying Wang ◽  
Xinran Li ◽  
Yue Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glucose Oxidase ◽  
Negative Correlation ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Endothelin 1 ◽  
Limulus Amoebocyte Lysate ◽  
And Control ◽  
Over Time

Abstract Horses (n = 20) were divided into 2 groups: oligofructose (OF)-induced equine laminitis group (group OF; n = 11) which received 10 g/kg b.w. of OF dissolved in 4 L water via nasogastric intubation, and control group (NS; n = 9) which received 4 L of saline. Blood was collected at 4 h intervals over 72 h study period and analysed by ELISA, kinetic limulus amoebocyte lysate assay, and glucose-oxidase methods. The level of insulin changed significantly in horses which received OF (P < 0.01); there was a significant negative correlation between the level of adiponectin and insulin over time. The results suggested that insulin may play an important role in the development of OF-induced equine laminitis by altering the level of endothelin-1 and nitric oxide.

The Limulus Amoebocyte Lysate Assay for Bacterial Endotoxins

1985 ◽  
Vol 13 (3) ◽  
pp. 180-192
Author(s):  
A. Christine McCartney
Keyword(s):  
Medical Devices ◽  
Limulus Amoebocyte Lysate ◽  
Bacterial Endotoxins

The Limulus amoeboecyte lysate (LAL) test not only provides a means for the detection and quantitation of endotoxin in parenteral preparations and medical devices to be used for the benefit of man without recourse to the use of animals, but also provides a more precise assay than the traditional rabbit test.

The Limulus Amoebocyte Lysate Assay and the Direct Epifluorescent Filter Technique as Estimators of Potential Shelf-life of Pasteurized Fluid Milk

1990 ◽  
Vol 53 (2) ◽  
pp. 151-153 ◽  
Author(s):  
ROBERT D. BYRNE ◽  
J. RUSSELL BISHOP
Keyword(s):  
Shelf Life ◽  
Benzalkonium Chloride ◽  
Detection Techniques ◽  
Filter Technique ◽  
Psychrotrophic Bacteria ◽  
Limulus Amoebocyte Lysate ◽  
Whole Milk ◽  
Before And After ◽  
Direct Epifluorescent Filter Technique ◽  
Fluid Milk

The Limulus Amoebocyte Lysate Assay, Direct Epifluorescent Filter Technique and modified Psychrotrophic Bacteria Count were used to indicate potential shelf-life of pasteurized fluid milk. Commercial whole milk samples, stored at 7°C, were analyzed for bacterial and biochemical parameters, as well as for potential shelf-life by daily sensory evaluation. Each sample was evaluated before and after the following preliminary incubations: milk alone, milk with benzalkonium chloride, milk and broth, and milk and broth with benzalkonium chloride. The Limulus Amoebocyte Lysate Assay, Direct Epifluorescent Filter Technique, and modified Psychrotrophic Bacteria Count in conjunction with the preliminary incubations, produced relatively high correlations to shelf-life (−0.78, −0.85, and −0.86 respectively). Thus, these bacterial detection techniques could be used as rapid methods of shelf-life estimation.

Salmonella Detection in Foods: Present Status and Research Needs for the Future

1984 ◽  
Vol 47 (1) ◽  
pp. 78-81 ◽  
Author(s):  
JEAN-YVES D'AOUST
Keyword(s):  
Membrane Filter ◽  
Fluorescent Antibody ◽  
Neutral Red ◽  
Test Material ◽  
Enzyme Linked Immunosorbent Assay ◽  
Selective Enrichment ◽  
Limulus Amoebocyte Lysate ◽  
Immunological Tests ◽  
Cost Efficient ◽  
Radiometric Technique

Standard cultural procedures generally require 4 to 5 d for presumptive evidence of Salmonella in foods. Attempts at greater method brevity have resulted in the use of selective enrichment cultures as test material for short immunological tests including fluorescent antibody (FA), enrichment serology (ES), enzyme-linked immunosorbent assay (ELISA), direct immunoenzyme (DI) and membrane filter-disc-immunoimmobilization (MFDI) assays. Nonimmunological tests such as the lysine-iron-cystine-neutral red (LICNR) broth and a 14C-dulcitol radiometric technique have also been applied to enrichment broth cultures. Sensitivity of short (4 to 6 h) incubation of selective enrichment broths has yet to be established. The need for rapid, cost-efficient preenrichment-dependent analytical schemes is clear. Investigations on the modification of the Limulus amoebocyte lysate (LAL) test to detect Salmonella cell wall antigens in preenrichment cultures or application of the ELISA, the hydrophobic-grid-membrane (HGMF) techniques or other rapid diagnostic tests to preenrichment cultures are indicated.

Effect of heparin sodium on limulus amoebocyte lysate preparations from two manufacturers

1991 ◽  
Vol 43 (6) ◽  
pp. 454-455
Author(s):  
N. V. NAIDU ◽  
I. J. KHALIFA ◽  
V. R. NALLARI ◽  
H. I. RAUT
Keyword(s):  
Heparin Sodium ◽  
Limulus Amoebocyte Lysate
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