Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

10.3791/58688 ◽  
2019 ◽  
Author(s):  
Romana Vidergar ◽  
Chiara Agostinis ◽  
Paola Zacchi ◽  
Alessandro Mangogna ◽  
Fleur Bossi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Complement Protein

Epithelial cell adhesion on films mimicking surface of polymeric scaffolds of artificial urethra compared to molecular modeling of integrin binding

2019 ◽  
Vol 34 (3) ◽  
pp. 280-290 ◽  
Author(s):  
Jakub Kollar ◽  
Andrea Morelli ◽  
Federica Chiellini ◽  
Stanislav Miertus ◽  
Dusan Bakos ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Polymer Films ◽  
Rational Design ◽  
Computational Study ◽  
Transmembrane Protein ◽  
Molecular Mechanic ◽  
Three Dimensional ◽  
Film Surface

In this study, a combined experimental and computational study of long-term human bladder epithelial cell line HBLAK adhesion and proliferation on five different polymeric surfaces, namely hyaluronic acid, amylose, collagen, polyhydroxybutyrate, and polylactic acid, was performed with the goal to understand the nature of the attraction between various surface materials and a simplified model of the cell surface (transmembrane protein integrin). These biodegradable polymers are frequently used as scaffolds for tissue engineering. During formation of the new tissue, the scaffold polymers are gradually replaced by the natural extracellular matrix of the proliferating cells. Cell adhesion and proliferation experiments were carried out employing thin polymer films prepared by solvent casting while for the computational approach three-dimensional molecular models of layers of ordered polymeric fibers were used as quasi-planar nano-sized models of polymeric surface patches. Experimental results indicated a good capability of amylose, polyhydroxybutyrate, and hyaluronic acid polymer films to foster cell adhesion. Proliferation experiment, carried out by incubating cells with the investigated polymer films for 72 h, showed that all the investigated polymers are able to sustain a good proliferation of HBLAK cells almost comparable to plain glass. Computational estimate of molecular mechanic interaction energies between three-dimensional models of polymeric films and the collagen-binding α2 I domain of the cell adhesion receptor integrin α2β1 confirmed elevated affinity to amylose and polyhydroxybutyrate that is related to higher polarity of function groups on the film surface as documented by the maps of molecular electrostatic potential. This combined experimental and modeling approach can contribute to rational design and surface modifications of polymeric material suitable for forming the scaffolds of human urethra that can be effectively colonized by stem cells.

Differential support of cell adhesion and growth by copolymers of polyurethane with hyaluronic acid

2013 ◽  
Vol 101 (10) ◽  
pp. 2870-2882 ◽  
Author(s):  
Amaliris Ruiz ◽  
Claire E. Flanagan ◽  
Kristyn S. Masters
Keyword(s):  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Differential Support

scholarly journals Hyaluronic Acid (Ha) Binding to Cd44 Activates Rac1 and Induces Lamellipodia Outgrowth

2000 ◽  
Vol 148 (6) ◽  
pp. 1159-1164 ◽  
Author(s):  
Snezhana Oliferenko ◽  
Irina Kaverina ◽  
J. Victor Small ◽  
Lukas A. Huber
Keyword(s):  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Recombinant Protein ◽  
Dominant Negative ◽  
Guanine Nucleotide ◽  
Adhesion Protein ◽  
Cell Edge ◽  
Gtp Binding ◽  
Cytoskeleton Rearrangements ◽  
Main Ligand

Both cell adhesion protein CD44 and its main ligand hyaluronic acid (HA) are thought to be involved in several processes ultimately requiring cytoskeleton rearrangements. Here, we show that the small guanine nucleotide (GTP)-binding protein, Rac1, can be activated upon HA binding to CD44. When applied locally to a passive cell edge, HA promoted the formation of lamellipodial protrusions in the direction of the stimulus. This process was inhibited by the prior injection of cells with dominant-negative N17Rac recombinant protein or by pretreatment of cells with monoclonal anti-CD44 antibodies, interfering with HA binding, implying the direct involvement of CD44 in signaling to Rac1.

scholarly journals Prostaglandin E2 activates complement protein CD55 to enhance cell adhesion in endometriosis

2019 ◽  
Vol 112 (3) ◽  
pp. e327
Author(s):  
Elliott G. Richards ◽  
Emily L. Esakov ◽  
Chad A. Braley ◽  
Jenna M. Rehmer ◽  
Jeffrey M. Goldberg ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Prostaglandin E2 ◽  
Complement Protein ◽  
Enhance Cell Adhesion

scholarly journals Complement Protein C1q Binds to Hyaluronic Acid in the Malignant Pleural Mesothelioma Microenvironment and Promotes Tumor Growth

2017 ◽  
Vol 8 ◽  
Author(s):  
Chiara Agostinis ◽  
Romana Vidergar ◽  
Beatrice Belmonte ◽  
Alessandro Mangogna ◽  
Leonardo Amadio ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Tumor Growth ◽  
Malignant Pleural Mesothelioma ◽  
Pleural Mesothelioma ◽  
Complement Protein

Cell adhesion and proteoglycans. I. The effect of exogenous proteoglycans on the attachment of chick embryo fibroblasts to tissue culture plastic and collagen

1979 ◽  
Vol 40 (1) ◽  
pp. 77-88
Author(s):  
P. Knox ◽  
P. Wells
Keyword(s):  
Tissue Culture ◽  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Chick Embryo ◽  
Structural Integrity ◽  
Chondroitin Sulphate ◽  
Cell Types ◽  
Tissue Culture Plastic ◽  
Keratan Sulphate ◽  
Covalently Linked

Proteoglycan was isolated from cartilage and freed from contaminating glycoproteins and hyaluronic acid. The macromolecule consists of a protein core covalently linked to a number of glycosaminoglycan side chains, namely chondroitin sulphate and keratan sulphate. This proteoglycan retards the attachment of a variety of cell types to tissue culture plastic and to collagen. Glycosaminoglycans alone, have no significant effect on rates of attachment. Similarly, trypsinized proteoglycan is without effect. It is concluded that the structural integrity of the proteoglycan macromolecule is essential for its effect on cell adhesion.

scholarly journals Cell adhesion molecules and hyaluronic acid as markers of inflammation, fibrosis and response to antiviral therapy in chronic hepatitis C patients

2001 ◽  
Vol 10 (5) ◽  
pp. 253-258 ◽  
Author(s):  
Esther Granot ◽  
Daniel Shouval ◽  
Yaffa Ashur
Keyword(s):  
Chronic Hepatitis ◽  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Hepatitis C ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecules ◽  
Response To Therapy ◽  
Markers Of Inflammation ◽  
Term Response

Objective:Cell adhesion molecules (intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1)) and hyaluronic acid, markers of inflammation and fibrosis were monitored in hepatitis C patients to determine whether changes in plasma levels, during antiviral treatment, can predict long-term response to therapy.Methods:In 55 patients with chronic hepatitis C virus (HCV), 33 treated with interferon (IFN) and 22 treated with IFN + ribavirin, sera was collected prior to treatment, at 3 + 6 months of therapy and 6 months post-treatment. Levels of ICAM-1, VCAM-1 and hyaluronic acid were correlated with alanine aminotransferase levels, HCV-RNA-polymerase chain reaction status and histological fibrosis scoring.Results:A decrease in ICAM-1 levels at 3 and 6 months of therapy, compared with pretreatment levels, was observed in responders to IFN + ribavirin therapy but this decrease in ICAM-1 levels was not evident following cessation of treatment. Hyaluronic acid levels, in both treatment groups, did not differ significantly between responders and non-responders. Hyaluronic acid levels did correlate, significantly, with degree of fibrosis whereas VCAM-1 levels were marginally increased only in patients with moderate (grade III) fibrosis.Conclusions:Monitoring of VCAM-1 and hyaluronic acid, during antiviral therapy, does not differentiate between responders and non-responders. A decrease in ICAM-1 levels during IFN + ribavirin treatmment is associated with response to therapy, and its efficacy in predicting long-term response should be further substantiated.

scholarly journals Role of Receptor for Hyaluronic Acid-mediated Motility (RHAMM) in Low Molecular Weight Hyaluronan (LMWHA)-mediated Fibrosarcoma Cell Adhesion

2011 ◽  
Vol 286 (44) ◽  
pp. 38509-38520 ◽  
Author(s):  
Katerina Kouvidi ◽  
Aikaterini Berdiaki ◽  
Dragana Nikitovic ◽  
Pavlos Katonis ◽  
Nikos Afratis ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Low Molecular Weight ◽  
Fibrosarcoma Cell

scholarly journals E‐selectin‐mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid

2017 ◽  
Vol 31 (11) ◽  
pp. 5078-5086 ◽  
Author(s):  
Daniel J. Shea ◽  
Yi W. Li ◽  
Kathleen J. Stebe ◽  
Konstantinos Konstantopoulos
Keyword(s):  
Pancreatic Cancer ◽  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cancer Cell Adhesion
Sign in / Sign up

Export Citation Format

Share Document