scholarly journals Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

10.3791/58134 ◽  
2018 ◽  
Author(s):  
Alexander Goedel ◽  
Dorota M. Zawada ◽  
Fangfang Zhang ◽  
Zhifen Chen ◽  
Alessandra Moretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Action Potential ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Ventricular Cardiomyocytes ◽  
Induced Pluripotent

Assessment of Drug Proarrhythmic Potential in Electrically Paced Human Induced Pluripotent Stem Cell–Derived Ventricular Cardiomyocytes Using Multielectrode Array

2020 ◽  
pp. 247255522095320
Author(s):  
Shuyun Bai ◽  
Junjie Pei ◽  
Kan Chen ◽  
Ya Zhao ◽  
Henghua Cao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Field Potential ◽  
Induced Pluripotent Stem Cell ◽  
Multielectrode Array ◽  
Drug Induced ◽  
Ventricular Cardiomyocytes ◽  
Wide Range ◽  
Beating Rate ◽  
Induced Pluripotent

Human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) have been widely used for the assessment of drug proarrhythmic potential through multielectrode array (MEA). HiPSC-CM cultures beat spontaneously with a wide range of frequencies, however, which could affect drug-induced changes in repolarization. Pacing hiPSC-CMs at a physiological heart rate more closely resembles the state of in vivo ventricular myocytes and permits the standardization of test conditions to improve consistency. In this study, we systematically investigated the time window of stable ion currents in high-purity hiPSC-derived ventricular cardiomyocytes (hiPSC-vCMs) and confirmed that these cells could be used to correctly predict the proarrhythmic risk of Comprehensive In Vitro Proarrhythmia Assay (CiPA) reference compounds. To evaluate drug proarrhythmic potentials at a physiological beating rate, we used a MEA to electrically pace hiPSC-vCMs, and we recorded regular field potential waveforms in hiPSC-vCMs treated with DMSO and 10 CiPA reference drugs. Prolongation of field potential duration was detected in cells after exposure to high- and intermediate-risk drugs; in addition, drug-induced arrhythmia-like events were observed. The results of this study provide a simple and feasible method to investigate drug proarrhythmic potentials in hiPSC-CMs at a physiological beating rate.

scholarly journals Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

2021 ◽  
Vol 2 (4) ◽  
pp. 100859
Author(s):  
Joe Z. Zhang ◽  
Shane Rui Zhao ◽  
Chengyi Tu ◽  
Paul Pang ◽  
Mao Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Action Potential ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals Deep phenotyping of human induced pluripotent stem cell–derived atrial and ventricular cardiomyocytes

JCI Insight ◽  
2018 ◽  
Vol 3 (12) ◽  
Author(s):  
Lukas Cyganek ◽  
Malte Tiburcy ◽  
Karolina Sekeres ◽  
Kathleen Gerstenberg ◽  
Hanibal Bohnenberger ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Ventricular Cardiomyocytes ◽  
Induced Pluripotent ◽  
Deep Phenotyping
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