scholarly journals In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

10.3791/58083 ◽  
2018 ◽  
Author(s):  
Ronald H.L. Li ◽  
Fern Tablin
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Microscopy ◽  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap

scholarly journals In vitro Demonstration and Quantification of Neutrophil Extracellular Trap Formation

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (13) ◽  
Author(s):  
Dongsheng Jiang ◽  
Mona Saffarzadeh ◽  
Karin Scharffetter-Kochanek
Keyword(s):  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap

scholarly journals TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Qian Ou ◽  
Jia-qi Fang ◽  
Zhe-sheng Zhang ◽  
Zhe Chi ◽  
Jie Fang ◽  
...  
Keyword(s):  
Innate Immune ◽  
Neutrophil Extracellular Trap ◽  
Mrna Levels ◽  
Peptidylarginine Deiminase ◽  
E Coli ◽  
Peptidylarginine Deiminase 4 ◽  
Trap Formation ◽  
Extracellular Trap ◽  
Innate Immune Evasion

AbstractTcpC is a multifunctional virulence factor of uropathogenic E. coli (UPEC). Neutrophil extracellular trap formation (NETosis) is a crucial anti-infection mechanism of neutrophils. Here we show the influence of TcpC on NETosis and related mechanisms. We show NETosis in the context of a pyelonephritis mouse model induced by TcpC-secreting wild-type E. coli CFT073 (CFT073wt) and LPS-induced in vitro NETosis with CFT073wt or recombinant TcpC (rTcpC)-treated neutrophils are inhibited. rTcpC enters neutrophils through caveolin-mediated endocytosis and inhibits LPS-induced production of ROS, proinflammatory cytokines and protein but not mRNA levels of peptidylarginine deiminase 4 (PAD4). rTcpC treatment enhances PAD4 ubiquitination and accumulation in proteasomes. Moreover, in vitro ubiquitination kit analyses show that TcpC is a PAD4-targetd E3 ubiquitin-ligase. These data suggest that TcpC inhibits NETosis primarily by serving as an E3 ligase that promotes degradation of PAD4. Our findings provide a novel mechanism underlying TcpC-mediated innate immune evasion.

scholarly journals Unmetabolized quetiapine exerts an in vitro effect on innate immune cells by modulating inflammatory response and neutrophil extracellular trap formation

2020 ◽  
Vol 131 ◽  
pp. 110497
Author(s):  
Bárbara Osmarin Turra ◽  
Fernanda Barbisan ◽  
Verônica Farina Azzolin ◽  
Cibele Ferreira Teixeira ◽  
Thamara Flores ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Immune Cells ◽  
Innate Immune ◽  
Neutrophil Extracellular Trap ◽  
Innate Immune Cells ◽  
Trap Formation ◽  
Extracellular Trap ◽  
Vitro Effect

scholarly journals MUC5B induces in vitro neutrophil extracellular trap formation: Implication in otitis media

2020 ◽  
Vol 5 (3) ◽  
pp. 536-545
Author(s):  
Stéphanie Val ◽  
Anna Krueger ◽  
Arman Hussain ◽  
Amarel Tomney ◽  
Yajun Chen ◽  
...  
Keyword(s):  
Otitis Media ◽  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap

scholarly journals Recombinant human thrombomodulin inhibits neutrophil extracellular trap formation in vitro

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Yasuyo Shimomura ◽  
Mika Suga ◽  
Naohide Kuriyama ◽  
Tomoyuki Nakamura ◽  
Toshikazu Sakai ◽  
...  
Keyword(s):  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap
Sign in / Sign up

Export Citation Format

Share Document