scholarly journals Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids

10.3791/58017 ◽  
2018 ◽  
Author(s):  
Kerstin Pieper ◽  
Kathi Gundermann ◽  
Lars Dietzel
Keyword(s):  
Light Harvesting ◽  
Cyclotella Meneghiniana ◽  
Thylakoid Lipids

scholarly journals Energy dissipation mechanisms in the FCPb light-harvesting complex of the diatom Cyclotella meneghiniana

2018 ◽  
Vol 1859 (10) ◽  
pp. 1151-1160 ◽  
Author(s):  
Huzifa M.A.M. Elnour ◽  
Lars Dietzel ◽  
Charusheela Ramanan ◽  
Claudia Büchel ◽  
Rienk van Grondelle ◽  
...  
Keyword(s):  
Energy Dissipation ◽  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Cyclotella Meneghiniana

scholarly journals The structure of FCPb, a light-harvesting complex in the diatom Cyclotella meneghiniana

2016 ◽  
Vol 135 (1-3) ◽  
pp. 203-211 ◽  
Author(s):  
Anja Röding ◽  
Egbert Boekema ◽  
Claudia Büchel
Keyword(s):  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Cyclotella Meneghiniana

scholarly journals Exploring the mechanism(s) of energy dissipation in the light harvesting complex of the photosynthetic algae Cyclotella meneghiniana

2014 ◽  
Vol 1837 (9) ◽  
pp. 1507-1513 ◽  
Author(s):  
Charusheela Ramanan ◽  
Rudi Berera ◽  
Kathi Gundermann ◽  
Ivo van Stokkum ◽  
Claudia Büchel ◽  
...  
Keyword(s):  
Energy Dissipation ◽  
Light Harvesting ◽  
Light Harvesting Complex ◽  
Cyclotella Meneghiniana

Role of Thylakoid Lipids in the Structural Flexibility of Lamellar Aggregates of the Isolated Light-Harvesting Chlorophylla/bComplex of Photosystem II†

Biochemistry ◽  
1998 ◽  
Vol 37 (12) ◽  
pp. 4169-4173 ◽  
Author(s):  
Ilian Simidjiev ◽  
Virginijus Barzda ◽  
László Mustárdy ◽  
Gyözö Garab
Keyword(s):  
Photosystem Ii ◽  
Light Harvesting ◽  
Structural Flexibility ◽  
Thylakoid Lipids

High-Resolution electron crystallography of the light-harvesting chlorophyll-a/b protein complex from chloroplast membranes

1990 ◽  
Vol 48 (1) ◽  
pp. 610-610
Author(s):  
Werner Kühlbrandt ◽  
Da Neng Wang ◽  
K.H. Downing
Keyword(s):  
High Resolution ◽  
Electron Diffraction ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Structural Integrity ◽  
Light Harvesting ◽  
Lhc Ii ◽  
Diffraction Patterns ◽  
Difference Fourier ◽  
2D Crystals

The light-harvesting chlorophyll-a/b protein complex (LHC-II) is the most abundant membrane protein in the chloroplasts of green plants where it functions as a molecular antenna of solar energy for photosynthesis. We have grown two-dimensional (2d) crystals of the purified, detergent-solubilized LHC-II . The crystals which measured 5 to 10 μm in diameter were stabilized for electron microscopy by washing with a 0.5% solution of tannin. Electron diffraction patterns of untilted 2d crystals cooled to 130 K showed sharp spots to 3.1 Å resolution. Spot-scan images of 2d crystals were recorded at 160 K with the Berkeley microscope . Images of untilted crystals were processed, using the unbending procedure by Henderson et al . A projection map of the complex at 3.7Å resolution was generated from electron diffraction amplitudes and high-resolution phases obtained by image processing .A difference Fourier analysis with the same image phases and electron diffraction amplitudes recorded of frozen, hydrated specimens showed no significant differences in the 3.7Å projection map. Our tannin treatment therefore does not affect the structural integrity of the complex.

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