scholarly journals Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

10.3791/57566 ◽  
2018 ◽  
Author(s):  
Markus Horsthemke ◽  
Janine Wilden ◽  
Anne C. Bachg ◽  
Peter J. Hanley
Keyword(s):  
3D Imaging ◽  
Time Lapse ◽  
Mouse Macrophages

scholarly journals Salmonella stimulate macrophage macropinocytosis and persist within spacious phagosomes.

1994 ◽  
Vol 179 (2) ◽  
pp. 601-608 ◽  
Author(s):  
C M Alpuche-Aranda ◽  
E L Racoosin ◽  
J A Swanson ◽  
S I Miller
Keyword(s):  
Bone Marrow ◽  
Plasma Membrane ◽  
Time Lapse ◽  
Membrane Ruffling ◽  
Mouse Macrophages ◽  
Constitutive Mutation ◽  
Microscopic Studies ◽  
Specific Igg ◽  
Salmonella Survival ◽  
Time Lapse Video

Light microscopic studies of phagocytosis showed that Salmonella typhimurium entered mouse macrophages enclosed in spacious phagosomes (SP). Viewed by time-lapse video microscopy, bone marrow-derived macrophages exposed to S. typhimurium displayed generalized plasma membrane ruffling and macropinocytosis. Phagosomes containing Salmonella were morphologically indistinguishable from macropinosomes. SP formation was observed after several methods of bacterial opsonization, although bacteria opsonized with specific IgG appeared initially in small phagosomes that later enlarged. In contrast to macropinosomes induced by growth factors, which shrink completely within 15 min, SP persisted in the cytoplasm, enlarging often by fusion with macropinosomes or other SP. A Salmonella strain containing a constitutive mutation in the phoP virulence regulatory locus (PhoPc) induced significantly fewer SP. Similar to Yersinia enterocolitica, PhoPc bacteria entered macrophages in close-fitting phagosomes, consistent with that expected for conventional receptor-mediated phagocytosis. These results suggest that formation of SP contributes to Salmonella survival and virulence.

scholarly journals Time Lapse 3D Imaging of Mineral Dissolution

2021 ◽  
Author(s):  
Chandra Winardhi ◽  
Jose Godinho ◽  
Jens Gutzmer ◽  
Gero Frisch
Keyword(s):  
3D Imaging ◽  
Time Lapse ◽  
Mineral Dissolution

scholarly journals Time-lapse 3D imaging by positron emission tomography of Cu mobilized in a soil column by the herbicide MCPA

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Johannes Kulenkampff ◽  
Madeleine Stoll ◽  
Marion Gründig ◽  
Alexander Mansel ◽  
Johanna Lippmann-Pipke ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
3D Imaging ◽  
Soil Column ◽  
Time Lapse ◽  
Emission Tomography ◽  
Positron Emission

Time-lapse 3D imaging of calcite precipitation in a microporous column

2018 ◽  
Vol 222 ◽  
pp. 156-170 ◽  
Author(s):  
Jose R.A. Godinho ◽  
Philip J. Withers
Keyword(s):  
3D Imaging ◽  
Time Lapse ◽  
Calcite Precipitation

scholarly journals In-vivo Optical Tomography of Small Scattering Specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Alicia Arranz ◽  
Di Dong ◽  
Shouping Zhu ◽  
Charalambos Savakis ◽  
Jie Tian ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
3D Imaging ◽  
Optical Tomography ◽  
Time Lapse

scholarly journals Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination

2012 ◽  
Vol 109 (14) ◽  
pp. 5311-5315 ◽  
Author(s):  
R. Fiolka ◽  
L. Shao ◽  
E. H. Rego ◽  
M. W. Davidson ◽  
M. G. L. Gustafsson
Keyword(s):  
3D Imaging ◽  
Time Lapse ◽  
Live Cells ◽  
Structured Illumination

A contractile activity that closes phagosomes in macrophages

1999 ◽  
Vol 112 (3) ◽  
pp. 307-316 ◽  
Author(s):  
J.A. Swanson ◽  
M.T. Johnson ◽  
K. Beningo ◽  
P. Post ◽  
M. Mooseker ◽  
...  
Keyword(s):  
Immunofluorescence Microscopy ◽  
Contractile Activity ◽  
Particle Surface ◽  
Microscopic Analysis ◽  
Time Lapse ◽  
Purse String ◽  
Mouse Macrophages ◽  
Butanedione Monoxime ◽  
Phosphoinositide 3 Kinase ◽  
Uncompetitive Inhibitor

Studies of Fc-mediated phagocytosis by mouse macrophages identified a contractile activity at the distal margins of forming phagosomes. Time-lapse video microscopic analysis of macrophages containing rhodamine-labeled actin and fluorescein dextran showed that actin was concentrated at the distal margins of closing phagosomes. Phagocytosis-related contractile activities were observed when one IgG-opsonized erythrocyte was engaged by two macrophages. Both cells extended pseudopodia until they met midway around the erythrocyte. It was then constricted and pulled into two phagosomes, which remained interconnected by a string of erythrocyte membrane. Butanedione monoxime, an uncompetitive inhibitor of class II and perhaps other myosins, and wortmannin and LY294002, inhibitors of phosphoinositide 3-kinase, prevented the constrictions without inhibiting the initial pseudopod extension. Immunofluorescence microscopy showed the presence of myosins IC, II, V and IXb in phagosomes. Of these, only myosin IC was concentrated around the strings connecting shared erythrocytes, suggesting that myosin IC mediates the purse-string-like contraction that closes phagosomes. The sequential processes of pseudopod extension and contraction can explain how macropinosomes and spacious phagosomes form without guidance from a particle surface.

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