scholarly journals Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

10.3791/57070 ◽  
2018 ◽  
Author(s):  
Daniela Baitan ◽  
Robin Schubert ◽  
Arne Meyer ◽  
Karsten Dierks ◽  
Markus Perbandt ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Crystals

Aqueous solubilization of hydrophobic supramolecular metal–organic nanocapsules

2014 ◽  
Vol 5 (6) ◽  
pp. 2554-2559 ◽  
Author(s):  
H. Kumari ◽  
S. R. Kline ◽  
J. L. Atwood
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Neutron Scattering ◽  
Metal Organic

Micelles of surfactant solubilized metal-seamed pyrogallol[4]arene based organic nanocapsules are synthesized and characterized using in situ neutron scattering and dynamic light scattering techniques, which show trends in sizes as a function of alkyl tails of pyrogallols and surfactants.

Vesicles made of PS-PI cyclic diblock copolymers: In situ freeze-drying cryo-TEM and dynamic light scattering experiments

2005 ◽  
Vol 128 ◽  
pp. 163 ◽  
Author(s):  
Jean-Luc Putaux ◽  
Edson Minatti ◽  
Christelle Lefebvre ◽  
Redouane Borsali ◽  
Michel Schappacher ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Freeze Drying ◽  
Diblock Copolymers

In Situ Dynamic-Light-Scattering Study of a Cellular Interface Arising During Directional Growth

1991 ◽  
Vol 15 (2) ◽  
pp. 197-202
Author(s):  
J.-M Laherrère ◽  
J.-C Tolédano ◽  
H Savary ◽  
R Mellet
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Directional Growth ◽  
Scattering Study

In Situ Dynamic Light Scattering Complementing Neutron Spin Echo Measurements on Protein Samples

2020 ◽  
Vol 14 (S1) ◽  
pp. S185-S189
Author(s):  
L. Balacescu ◽  
F. Vögl ◽  
S. Staringer ◽  
V. Ossovyi ◽  
G. Brandl ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Spin Echo ◽  
Neutron Spin ◽  
Neutron Spin Echo

scholarly journals Do protein crystals nucleate within dense liquid clusters?

2015 ◽  
Vol 71 (7) ◽  
pp. 815-822 ◽  
Author(s):  
Dominique Maes ◽  
Maria A. Vorontsova ◽  
Marco A. C. Potenza ◽  
Tiziano Sanvito ◽  
Mike Sleutel ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Glucose Isomerase ◽  
Crystal Nucleation ◽  
Protein Crystal ◽  
Protein Crystals ◽  
Liquid Clusters ◽  
Protein Rich ◽  
Dense Liquid ◽  
Step Mechanism

Protein-dense liquid clusters are regions of high protein concentration that have been observed in solutions of several proteins. The typical cluster size varies from several tens to several hundreds of nanometres and their volume fraction remains below 10−3of the solution. According to the two-step mechanism of nucleation, the protein-rich clusters serve as locations for and precursors to the nucleation of protein crystals. While the two-step mechanism explained several unusual features of protein crystal nucleation kinetics, a direct observation of its validity for protein crystals has been lacking. Here, two independent observations of crystal nucleation with the proteins lysozyme and glucose isomerase are discussed. Firstly, the evolutions of the protein-rich clusters and nucleating crystals were characterized simultaneously by dynamic light scattering (DLS) and confocal depolarized dynamic light scattering (cDDLS), respectively. It is demonstrated that protein crystals appear following a significant delay after cluster formation. The cDDLS correlation functions follow a Gaussian decay, indicative of nondiffusive motion. A possible explanation is that the crystals are contained inside large clusters and are driven by the elasticity of the cluster surface. Secondly, depolarized oblique illumination dark-field microscopy reveals the evolution from liquid clusters without crystals to newly nucleated crystals contained in the clusters to grown crystals freely diffusing in the solution. Collectively, the observations indicate that the protein-rich clusters in lysozyme and glucose isomerase solutions are locations for crystal nucleation.

Particle Size Measurement in Optical Waveguide Manufacturing Torches Using Dynamic Light Scattering

1988 ◽  
Vol 117 ◽  
Author(s):  
J. R. Bautista ◽  
Eugene Potkay ◽  
Daria L. Scatton
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Optical Waveguide ◽  
Low Frequency ◽  
Correlation Spectroscopy ◽  
Industrial Systems ◽  
Deposition Processes ◽  
Flame Hydrolysis Deposition

AbstractThe characterization of silica “sooting” torches used in optical waveguide manufacture by external flame-hydrolysis deposition processes such as Outside Vapor Deposition (OVD) and Vapor-phase Axial Deposition (VAD) prflents a formid.ble high temperature environment at > 2000°C in the presence of > 1012 particles/cm3 of silica. This paper describes the first application of Dynamic Light Scattering (DLS) to probe particle sizes in an actual lightguide burner following the “triple-flame” VAD design. DLS, also termed Photon Correlation Spectroscopy (PCS) or Diffusion Broadening Spectroscopy (DBS), was previously identified as a viable diagnostic tool for in-situ particle measurements in silica-sooting flames by W. L. Flower [1] and through an earlier collaboration between AT&T and the Sandia Combustion Research Facility. In applying DLS, however, our experience with the VAD torch reveals that a specialized experimental method will undoubtedly be required to deal with the inevitable presence of low frequency system instabilities in industrial systems.

scholarly journals Monitoring and Scoring Counter-Diffusion Protein Crystallization Experiments in Capillaries by in situ Dynamic Light Scattering

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e33545 ◽  
Author(s):  
Dominik Oberthuer ◽  
Emilio Melero-García ◽  
Karsten Dierks ◽  
Arne Meyer ◽  
Christian Betzel ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Crystallization ◽  
Crystallization Experiments ◽  
Counter Diffusion
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