scholarly journals A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

10.3791/56785 ◽  
2018 ◽  
Author(s):  
Patrick Ming-Kuen Tang ◽  
Philip Chiu-Tsun Tang ◽  
Jeff Yat-Fai Chung ◽  
Jessica Shuk Chun Hung ◽  
Qing-Ming Wang ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Mass Production ◽  
Killer Cells ◽  
Free System

In-vitro activation of natural killer cells from regional lymph nodes of melanoma patients with interleukin-2 and interleukin-15

2015 ◽  
Vol 25 (1) ◽  
pp. 22-34 ◽  
Author(s):  
Ana M. Vuletić ◽  
Irena P. Jovanić ◽  
Vladimir B. Jurišić ◽  
Zorka M. Milovanović ◽  
Srđan S. Nikolić ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Lymph Nodes ◽  
Natural Killer ◽  
Interleukin 2 ◽  
Regional Lymph Nodes ◽  
Killer Cells ◽  
In Vitro Activation ◽  
Interleukin 15 ◽  
Melanoma Patients

Intrinsic Cytotoxicity of Natural Killer Cells to Pancreatic Islets In Vitro

Diabetes ◽  
1990 ◽  
Vol 39 (7) ◽  
pp. 836-843 ◽  
Author(s):  
N. Akamura ◽  
B. A. Woda ◽  
A. Tafuri ◽  
D. L. Greiner ◽  
C. W. Reynolds ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Pancreatic Islets ◽  
Natural Killer ◽  
Killer Cells

scholarly journals Production of interferon-γ by natural killer cells and aging in chronic human schistosomiasis

2004 ◽  
Vol 13 (5-6) ◽  
pp. 327-333 ◽  
Author(s):  
E. Speziali ◽  
J. Bethony ◽  
O. Martins-Filho ◽  
L. A. O. Fraga ◽  
D. S. Lemos ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Infection Intensity ◽  
The Elderly ◽  
Interferon Γ ◽  
Older Individuals ◽  
Killer Cells ◽  
Human Schistosomiasis ◽  
Ifn Γ

BACKGROUNG: Aging is associated with several alterations in the phenotype, repertoire and activation status of lymphocytes as well as in the cytokine profile produced by these cells. As a lifelong condition, chronic parasitic diseases such as human schistosomiasis overlaps with the aging process and no systematic study has yet addressed the changes in immune response during infection withSchistosoma mansoniin older individuals.Aim: Herein we study the influence of immunological alterations brought about by senescence in the course of schistosomiasis.Materials and methods: Individuals 10-95 years of age, from both sexes, from an endemic area forS. mansoniinfection were matched by intensity of infection as measured by egg counts. We analyzed, as a parameter, cytokine expression by lymphocytes and natural killer cells afterin vitrostimulation with soluble egg antigen and soluble worm antigen using flow cytometry.Results: We demonstrated that the frequency of CD16+interferon-γ (IFN-γ)+natural killer cells in negative individuals over the age of 70 years is significantly higher than in positive individuals afterin vitrostimulation withS. mansoniantigen extracts. The frequency of these cells is increased in all individuals over the age of 50 years and only declines in positive individuals after 70 years of age. Analysis of either CD4?or CD8?cells after antigen stimulation show no significant increase in frequency of IFN-γ in negative or in positive individuals of this age group, suggesting that the effect on CD16+cells is not T-cell dependent.Conclusion: Since production of IFN-γ has been related to resistance to schistosome infection, our data suggest that age-associated changes in CD16+cells may play a role in controlling infection intensity in the elderly inS. mansoniendemic areas of Brazil.

scholarly journals Potential Therapeutic Effect of Natural Killer Cells on Doxorubicin-Resistant Breast Cancer Cells In Vitro

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136209 ◽  
Author(s):  
Mi-Hye Hwang ◽  
Xiu Juan Li ◽  
Jung Eun Kim ◽  
Shin Young Jeong ◽  
Sang-Woo Lee ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Natural Killer Cells ◽  
Cancer Cells ◽  
Natural Killer ◽  
Therapeutic Effect ◽  
Breast Cancer Cells ◽  
Killer Cells
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