scholarly journals Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

10.3791/56747 ◽  
2017 ◽  
Author(s):  
Caroline Haupt ◽  
Tommy Hofmann ◽  
Sabine Wittig ◽  
Susann Kostmann ◽  
Argyris Politis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Complexes ◽  
Intact Protein ◽  
Cross Linking ◽  
Protein Assemblies ◽  
Subunit Protein ◽  
Chemical Cross Linking

scholarly journals Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes

2014 ◽  
Vol 111 (26) ◽  
pp. 9455-9460 ◽  
Author(s):  
Alexander Leitner ◽  
Lukasz A. Joachimiak ◽  
Pia Unverdorben ◽  
Thomas Walzthoeni ◽  
Judith Frydman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Complexes ◽  
Cross Linking ◽  
Acidic Residues ◽  
Chemical Cross Linking

Chemical Cross-linking and Mass Spectrometry for the Structural Analysis of Protein Assemblies

2013 ◽  
Vol 66 (7) ◽  
pp. 749 ◽  
Author(s):  
Antonio N. Calabrese ◽  
Tara L. Pukala
Keyword(s):  
Mass Spectrometry ◽  
Structural Biology ◽  
Structural Information ◽  
Cross Linking ◽  
Spectrometric Analysis ◽  
Protein Assemblies ◽  
Cellular Functions ◽  
The Past ◽  
And Function ◽  
Chemical Cross Linking

Cellular functions are performed and regulated at a molecular level by the coordinated action of intricate protein assemblies, and hence the study of protein folding, structure, and interactions is vital to the appreciation and understanding of complex biological problems. In the past decade, continued development of chemical cross-linking methodologies combined with mass spectrometry has seen this approach develop to enable detailed structural information to be elucidated for protein assemblies often intractable by traditional structural biology methods. In this review article, we describe recent advances in reagent design, cross-linking protocols, mass spectrometric analysis, and incorporation of cross-linking constraints into structural models, which are contributing to overcoming the intrinsic challenges of the cross-linking method. We also highlight pioneering applications of chemical cross-linking mass spectrometry approaches to the study of structure and function of protein assemblies.

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