scholarly journals Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)

10.3791/56623 ◽  
2017 ◽  
Author(s):  
Priya Crosby ◽  
Nathaniel P. Hoyle ◽  
John S. O'Neill
Keyword(s):  
Flexible Measurement ◽  
Bioluminescent Reporters

Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo

2005 ◽  
Vol 10 (4) ◽  
pp. 041210 ◽  
Author(s):  
Hui Zhao ◽  
Timothy C. Doyle ◽  
Olivier Coquoz ◽  
Flora Kalish ◽  
Bradley W. Rice ◽  
...  
Keyword(s):  
Emission Spectra ◽  
Mammalian Tissue ◽  
Bioluminescent Reporters

scholarly journals Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors

2019 ◽  
Vol 12 (1) ◽  
pp. 129-150 ◽  
Author(s):  
Hsien-Wei Yeh ◽  
Hui-Wang Ai
Keyword(s):  
Excited State ◽  
External Excitation ◽  
Excitation Light ◽  
Fluorescent Reporters ◽  
Fluorescence Measurements ◽  
Chemiluminescent Sensors ◽  
Biomedical Fields ◽  
Bioluminescent Reporters

Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Lastly, we discuss chemiluminescent sensors that produce photons in the absence of luciferases. This review aims to explore fundamentals and experimental insights and to emphasize the yet-to-be-reached potential of next-generation luminescent reporters and biosensors.

Logistic Regression with Exposure Biomarkers and Flexible Measurement Error

Biometrics ◽  
2006 ◽  
Vol 63 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Elizabeth A. Sugar ◽  
Ching-Yun Wang ◽  
Ross L. Prentice
Keyword(s):  
Logistic Regression ◽  
Measurement Error ◽  
Exposure Biomarkers ◽  
Flexible Measurement

The Design of Intelligent Measurement System Based on Time-Triggered Embedded System

2014 ◽  
Vol 643 ◽  
pp. 196-200
Author(s):  
Feng Huang ◽  
Xin Bian ◽  
Guo Rong Cao
Keyword(s):  
Embedded System ◽  
Data Storage ◽  
Measurement System ◽  
Data Transmission ◽  
Experimental Results ◽  
Control Data ◽  
Intelligent Measurement ◽  
Measurement Control ◽  
The Stability ◽  
Flexible Measurement

An intelligent measurement system is introduced, which is based on time-triggered embedded system with the characteristics of multitask and multiparameter in the process of measuring parameters. The system is consist of MCU, USB and the time-triggered structure. The predictability makes the system avoid the interrupt loss. The stability and reliability of the system has been improved. The experimental results show that the system not only has the characteristics of flexible measurement control, data transmission, and data storage, but also can analysis the field parameters.

scholarly journals Construction of Mobilizable Mini-Tn7Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy PromoterluxReporter Analysis

2013 ◽  
Vol 79 (13) ◽  
pp. 4149-4153 ◽  
Author(s):  
F. Heath Damron ◽  
Elizabeth S. McKenney ◽  
Mariette Barbier ◽  
George W. Liechti ◽  
Herbert P. Schweizer ◽  
...  
Keyword(s):  
Host Range ◽  
Single Copy ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Bioluminescent Reporters

ABSTRACTWe describe the construction of mini-Tn7-based broad-host-range vectors encodingluxgenes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-luxintegration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

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