scholarly journals Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

10.3791/56604 ◽  
2018 ◽  
Author(s):  
Andrea Brigitta Alber ◽  
David Michael Suter
Keyword(s):  
Cell Culture ◽  
Fluorescence Microscopy ◽  
Protein Degradation ◽  
Single Cell ◽  
Time Lapse ◽  
Adherent Cell ◽  
Cell Quantification ◽  
Degradation Rates ◽  
Adherent Cell Culture

In Situ Single‐Cell Western Blot on Adherent Cell Culture

2019 ◽  
Vol 131 (39) ◽  
pp. 14067-14072 ◽  
Author(s):  
Yizhe Zhang ◽  
Isao Naguro ◽  
Amy E. Herr
Keyword(s):  
Cell Culture ◽  
Western Blot ◽  
Single Cell ◽  
Adherent Cell ◽  
Adherent Cell Culture

Measurement of Cell–Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture

2018 ◽  
Vol 90 (15) ◽  
pp. 9637-9643 ◽  
Author(s):  
Sifeng Mao ◽  
Qiang Zhang ◽  
Haifang Li ◽  
Qiushi Huang ◽  
Mashooq Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Single Cell ◽  
Adherent Cell ◽  
Matrix Adhesion ◽  
Cell Matrix ◽  
Adherent Cell Culture ◽  
Cell Matrix Adhesion

scholarly journals Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level

2011 ◽  
Vol 6 (12) ◽  
pp. 1847-1859 ◽  
Author(s):  
Felipe Ortega ◽  
Marcos R Costa ◽  
Tatiana Simon-Ebert ◽  
Timm Schroeder ◽  
Magdalena Götz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Adherent Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Adult Neural Stem Cells ◽  
Subependymal Zone ◽  
Adherent Cell Culture

scholarly journals In Situ Single‐Cell Western Blot on Adherent Cell Culture

2019 ◽  
Vol 58 (39) ◽  
pp. 13929-13934 ◽  
Author(s):  
Yizhe Zhang ◽  
Isao Naguro ◽  
Amy E. Herr
Keyword(s):  
Cell Culture ◽  
Western Blot ◽  
Single Cell ◽  
Adherent Cell ◽  
Adherent Cell Culture

scholarly journals Use of a non-adherent cell culture system for testing the effect of 2′,3′-dideoxyinosine againstCryptosporidium parvum

1999 ◽  
Vol 176 (1) ◽  
pp. 235-240
Author(s):  
Philippe Lawton ◽  
Carine Hejl ◽  
Marie-Elisabeth Sarciron ◽  
Roselyne Mancassola ◽  
Muriel Naciri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Adherent Cell ◽  
Cell Culture System ◽  
Adherent Cell Culture

NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

scholarly journals AFM and Fluorescence Microscopy of Single Cells with Simultaneous Mechanical Stimulation via Electrically Stretchable Substrates

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4131
Author(s):  
Natalia Becerra ◽  
Barbara Salis ◽  
Mariateresa Tedesco ◽  
Susana Moreno Flores ◽  
Pasquale Vena ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Spatial Resolution ◽  
Single Cells ◽  
Optical Microscope ◽  
Average Cell ◽  
Atomic Force ◽  
Cell Stretcher

We have developed a novel experimental set-up that simultaneously, (i) applies static and dynamic deformations to adherent cells in culture, (ii) allows the visualization of cells under fluorescence microscopy, and (iii) allows atomic force microscopy nanoindentation measurements of the mechanical properties of the cells. The cell stretcher device relies on a dielectric elastomer film that can be electro-actuated and acts as the cell culture substrate. The shape and position of the electrodes actuating the film can be controlled by design in order to obtain specific deformations across the cell culture chamber. By using optical markers we characterized the strain fields under different electrode configurations and applied potentials. The combined setup, which includes the cell stretcher device, an atomic force microscope, and an inverted optical microscope, can assess in situ and with sub-micron spatial resolution single cell topography and elasticity, as well as ion fluxes, during the application of static deformations. Proof of performance on fibroblasts shows a reproducible increase in the average cell elastic modulus as a response to applied uniaxial stretch of just 4%. Additionally, high resolution topography and elasticity maps on a single fibroblast can be acquired while the cell is deformed, providing evidence of long-term instrumental stability. This study provides a proof-of-concept of a novel platform that allows in situ and real time investigation of single cell mechano-transduction phenomena with sub-cellular spatial resolution.

Laminin-adherent versus suspension-non-adherent cell culture conditions for the isolation of cancer stem cells in the DAOY medulloblastoma cell line

Tumor Biology ◽  
2016 ◽  
Vol 37 (9) ◽  
pp. 12359-12370 ◽  
Author(s):  
Javier de la Rosa ◽  
Ander Sáenz Antoñanzas ◽  
Mehdi H. Shahi ◽  
Bárbara Meléndez ◽  
Juan A. Rey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Culture Conditions ◽  
Adherent Cell ◽  
Medulloblastoma Cell ◽  
Medulloblastoma Cell Line ◽  
Adherent Cell Culture ◽  
Cell Culture Conditions

Measuring bacterial adaptation dynamics at the single-cell level using a microfluidic chemostat and time-lapse fluorescence microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (20) ◽  
pp. 5254-5262 ◽  
Author(s):  
Zhicheng Long ◽  
Anne Olliver ◽  
Elisa Brambilla ◽  
Bianca Sclavi ◽  
Marco Cosentino Lagomarsino ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Cell ◽  
Time Lapse ◽  
Single Cell Level ◽  
Cell Level ◽  
Gfp Expression ◽  
Bacterial Adaptation ◽  
E Coli ◽  
Cell Dimensions

We grewE. coliin a microfluidic chemostat and monitored the dynamics of cell dimensions and reporter GFP expression in individual cells during nutritional upshift or downshift.

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