scholarly journals Serum and Plasma Copy Number Detection Using Real-time PCR

10.3791/56502 ◽  
2017 ◽  
Author(s):  
Samanta Salvi ◽  
Vincenza Conteduca ◽  
Filippo Martignano ◽  
Giorgia Gurioli ◽  
Daniele Calistri ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Copy Number Detection

scholarly journals Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132954 ◽  
Author(s):  
Bruce Humphrey ◽  
Neil McLeod ◽  
Carrie Turner ◽  
J. Mark Sutton ◽  
Paul M. Dark ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Low Copy Number ◽  
Copy Number Detection ◽  
Clinically Relevant Bacteria

Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum)

2008 ◽  
Vol 375 (1) ◽  
pp. 150-152 ◽  
Author(s):  
Cheng Xin Yi ◽  
Jun Zhang ◽  
Ka Man Chan ◽  
Xiao Kun Liu ◽  
Yan Hong
Keyword(s):  
Gossypium Hirsutum ◽  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Transgene Copy Number

TaqMan real-time PCR quantification strategy of CYP2D6 gene copy number for the LightCycler 2.0

2009 ◽  
Vol 403 (1-2) ◽  
pp. 207-211 ◽  
Author(s):  
Duc L. Nguyen ◽  
Julia Staeker ◽  
Barbara Laika ◽  
Werner Steimer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Cyp2d6 Gene

The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR

2006 ◽  
Vol 65 (3) ◽  
pp. 476-487 ◽  
Author(s):  
Miguel A. Providenti ◽  
Jason M. O'Brien ◽  
Robyn J. Ewing ◽  
E. Suzanne Paterson ◽  
Myron L. Smith
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Sybr Green ◽  
Quantitative Real Time Pcr ◽  
Genetic Elements

scholarly journals Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Runa M Grimholt ◽  
Petter Urdal ◽  
Olav Klingenberg ◽  
Armin P Piehler
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Globin Gene ◽  
Copy Number Variations ◽  
Globin Genes ◽  
Quantitative Real Time Pcr ◽  
Human Genetic Disease ◽  
Large Deletions ◽  
Wide Range

Abstract Background Alpha-thalassemia is the most common human genetic disease worldwide. Copy number variations in the form of deletions of α-globin genes lead to α-thalassemia while duplications of α-globin genes can cause a severe phenotype in β-thalassemia carriers due to accentuation of globin chain imbalance. It is important to have simple and reliable methods to identify unknown or rare deletions and duplications in cases in which thalassemia is suspected but cannot be confirmed by multiplex gap-PCR. Here we describe a copy number variation assay to detect deletions and duplications in the α-globin gene cluster (HBA-CNV). Results Quantitative real-time PCR was performed using four TaqMan® assays which specifically amplify target sequences representing both the α-globin genes, the –α3.7 deletion and the HS-40 region. The copy number for each target was determined by the 2-ΔΔCq method. To validate our method, we compared the HBA-CNV method with traditional gap-PCR in 108 samples from patients referred to our laboratory for hemoglobinopathy evaluation. To determine the robustness of the four assays, we analyzed samples with and without deletions diluted to obtain different DNA concentrations. The HBA-CNV method identified the correct copy numbers in all 108 samples. All four assays showed the correct copy number within a wide range of DNA concentrations (3.2-100 ng/μL), showing that it is a robust and reliable method. By using the method in routine diagnostics of hemoglobinopathies we have also identified several deletions and duplications that are not detected with conventional gap-PCR. Conclusions HBA-CNV is able to detect all known large deletions and duplications affecting the α-globin genes, providing a flexible and simple workflow with rapid and reliable results.

Quantification of the Transcripts Encoding Different Forms of AChE in Various Cell Types: Real-Time PCR Coupled with Standards in Revealing the Copy Number

2014 ◽  
Vol 53 (3) ◽  
pp. 461-468 ◽  
Author(s):  
Cathy W.C. Bi ◽  
Wilson K.W. Luk ◽  
María-Letizia Campanari ◽  
Yuen H. Liu ◽  
Li Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Cell Types

scholarly journals Photo-Induced Electron Transfer Real-Time PCR for Detection ofPlasmodium falciparum plasmepsin 2Gene Copy Number

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Samaly Santos Souza ◽  
Mariangela L'Episcopia ◽  
Carlo Severini ◽  
Venkatachalam Udhayakumar ◽  
Naomi W. Lucchi
Keyword(s):  
Electron Transfer ◽  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy ◽  
Content Type ◽  
Copy Numbers ◽  
Ofplasmodium Falciparum ◽  
Photo Induced Electron Transfer ◽  
Gene Copy Numbers

ABSTRACTPiperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in theplasmepsin 2and3gene copy numbers has been associated with decreased susceptibility ofPlasmodium falciparumto piperaquine in Cambodia. Here, we developed a photo-induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of theP. falciparumplasmepsin 2gene (PfPM2) that can be used in countries whereP. falciparumis endemic to enhance molecular surveillance.

scholarly journals Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR

Plasmid ◽  
2008 ◽  
Vol 60 (2) ◽  
pp. 118-124 ◽  
Author(s):  
Nathalie Turgeon ◽  
Christian Laflamme ◽  
Jim Ho ◽  
Caroline Duchaine
Keyword(s):  
Real Time ◽  
Bacterial Growth ◽  
Real Time Pcr ◽  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy
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