scholarly journals Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

10.3791/56404 ◽  
2017 ◽  
Author(s):  
Max R Salick ◽  
Michael F Wells ◽  
Kevin Eggan ◽  
Ajamete Kaykas
Keyword(s):  
Stem Cell ◽  
Virus Infection ◽  
Human Brain ◽  
Zika Virus ◽  
Zika Virus Infection

The P-MAPA immunomodulator partially prevents apoptosis induced by Zika virus infection in THP-1 cells

2020 ◽  
Vol 21 ◽  
Author(s):  
Morganna C. Lima ◽  
Elisa A. N. Azevedo ◽  
Clarice N. L. de Morais ◽  
Larissa I. O. de Sousa ◽  
Bruno M. Carvalho ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Virus Infection ◽  
Virus Replication ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Caspase 3 ◽  
Neurological Complications ◽  
Zika Virus Infection

Background: Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology. Objective: To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells. Methods: THP-1 cells were subjected at Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin+/ PI+ and caspase 3/ 7+ staining by flow cytometry. In addition, the virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively. Results: We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation. Conclusions: Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibits the effects of Zika virus infection in mammalian cells.

scholarly journals JMX0207, a Niclosamide Derivative with Improved Pharmacokinetics, Suppresses Zika Virus Infection Both In Vitro and In Vivo

2020 ◽  
Vol 6 (10) ◽  
pp. 2616-2628 ◽  
Author(s):  
Zhong Li ◽  
Jimin Xu ◽  
Yuekun Lang ◽  
Xiaoyu Fan ◽  
Lili Kuo ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Zika Virus Infection

scholarly journals MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection

2018 ◽  
Vol 12 (1) ◽  
pp. e0006154 ◽  
Author(s):  
Dominic Paquin-Proulx ◽  
Vivian I. Avelino-Silva ◽  
Bianca A. N. Santos ◽  
Nathália Silveira Barsotti ◽  
Fabiana Siroma ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Dengue Virus Infection ◽  
Mait Cells ◽  
Zika Virus Infection

Beneficial in vitro effects of the passion fruit seed extract against placental Zika virus infection

Placenta ◽  
2021 ◽  
Vol 112 ◽  
pp. e42
Author(s):  
Eloiza Tanabe ◽  
Jaqueline Santos ◽  
Keyla Pires ◽  
Monique Nova ◽  
Karen Borbely ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Seed Extract ◽  
Passion Fruit ◽  
Zika Virus Infection ◽  
In Vitro Effects

scholarly journals Autopsy and Postmortem Studies Are Concordant: Pathology of Zika Virus Infection Is Neurotropic in Fetuses and Infants With Microcephaly Following Transplacental Transmission

2016 ◽  
Vol 141 (1) ◽  
pp. 68-72 ◽  
Author(s):  
David A. Schwartz
Keyword(s):  
Virus Infection ◽  
Brain Damage ◽  
Vertical Transmission ◽  
Zika Virus ◽  
In Vivo Studies ◽  
Cytopathic Effects ◽  
Zika Virus Infection ◽  
Postmortem Studies

Context.—Pathology studies have been important in concluding that Zika virus infection occurring in pregnant women can result in vertical transmission of the agent from mother to fetus. Fetal and infant autopsies have provided crucial direct evidence that Zika virus can infect an unborn child, resulting in microcephaly, other malformations, and, in some cases, death. Objective.—To better understand the etiologic role and mechanism(s) of Zika virus in causing birth defects such as microcephaly, this communication analyzes the spectrum of clinical and autopsy studies reported from fetuses and infants who developed intrauterine Zika virus infection, and compares these findings with experimental data related to Zika virus infection. Design.—Retrospective analysis of reported clinical, autopsy, pathology, and related postmortem studies from 9 fetuses and infants with intrauterine Zika virus infection and microcephaly. Results.—All fetuses and infants examined demonstrated an overlapping spectrum of gross and microscopic neuropathologic abnormalities. Direct cytopathic effects of infection by the Zika virus were confined to the brain; in cases where other organs were evaluated, no direct viral effects were identified. Conclusions.—There is concordance of the spectrum of brain damage, reinforcing previous data indicating that the Zika virus has a strong predilection for cells of the fetal central nervous system following vertical transmission. The occurrence of additional congenital abnormalities suggests that intrauterine brain damage from Zika virus interferes with normal fetal development, resulting in fetal akinesia. Experimental in vitro and in vivo studies of Zika virus infection corroborate the human autopsy findings of neural specificity.

In vitro study of Zika virus infection in boar semen

2017 ◽  
Vol 162 (10) ◽  
pp. 3209-3213 ◽  
Author(s):  
Natthanej Luplertlop ◽  
San Suwanmanee ◽  
Sumate Ampawong ◽  
Sompong Vongpunsawad ◽  
Yong Poovorawan
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Zika Virus Infection ◽  
Boar Semen

scholarly journals Zika virus infection dysregulates human neural stem cell growth and inhibits differentiation into neuroprogenitor cells

2017 ◽  
Vol 8 (10) ◽  
pp. e3106-e3106 ◽  
Author(s):  
Pradip Devhare ◽  
Keith Meyer ◽  
Robert Steele ◽  
Ratna B Ray ◽  
Ranjit Ray
Keyword(s):  
Stem Cell ◽  
Cell Growth ◽  
Virus Infection ◽  
Neural Stem Cell ◽  
Zika Virus ◽  
Human Neural Stem Cell ◽  
Zika Virus Infection

scholarly journals Punica granatum Leaf Ethanolic Extract and Ellagic Acid as Inhibitors of Zika Virus Infection

Planta Medica ◽  
2020 ◽  
Vol 86 (18) ◽  
pp. 1363-1374
Author(s):  
Stefano Acquadro ◽  
Andrea Civra ◽  
Cecilia Cagliero ◽  
Arianna Marengo ◽  
Massimo Rittà ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Ellagic Acid ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Therapeutic Interventions ◽  
Zika Virus Infection ◽  
Neurological Effects

AbstractZika virus, an arthropod-borne flavivirus, is an emerging healthcare threat worldwide. Zika virus is responsible for severe neurological effects, such as paralytic Guillain-Barrè syndrome, in adults, and also congenital malformations, especially microcephaly. No specific antiviral drugs and vaccines are currently available, and treatments are palliative, but medicinal plants show great potential as natural sources of anti-Zika phytochemicals. This study deals with the investigation of the composition, cytotoxicity, and anti-Zika activity of Punica granatum leaf ethanolic extract, fractions, and phytoconstituents. P. granatum leaves were collected from different areas in Italy and Greece in different seasons. Crude extracts were analyzed and fractionated, and the pure compounds were isolated. The phytochemical and biomolecular fingerprint of the pomegranate leaves was determined. The antiviral activities of the leaf extract, fractions, and compounds were investigated against the MR766 and HPF2013 Zika virus strains in vitro. Both the extract and its fractions were found to be active against Zika virus infection. Of the compounds isolated, ellagic acid showed particular anti-Zika activities, with EC50 values of 30.86 µM for MR766 and 46.23 µM for HPF2013. The mechanism of action was investigated using specific antiviral assays, and it was demonstrated that ellagic acid was primarily active as it prevented Zika virus infection and was able to significantly reduce Zika virus progeny production. Our data demonstrate the anti-Zika activity of pomegranate leaf extract and ellagic acid for the first time. These findings identify ellagic acid as a possible anti-Zika candidate compound that can be used for preventive and therapeutic interventions.

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