scholarly journals Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

10.3791/56084 ◽  
2017 ◽  
Author(s):  
Elena K. Schneider ◽  
Felisa Reyes-Ortega ◽  
Jian Li ◽  
Tony Velkov
Keyword(s):  
Cystic Fibrosis ◽  
High Throughput ◽  
Biological Fluids ◽  
Clinical Samples ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”

2021 ◽  
Vol 2 ◽  
Author(s):  
Beatriz B. Oliveira ◽  
Bruno Veigas ◽  
Pedro Viana Baptista
Keyword(s):  
High Throughput ◽  
Gold Standard ◽  
Dna Amplification ◽  
Standard Technique ◽  
Isothermal Amplification ◽  
Nucleic Acid Amplification ◽  
Clinical Samples ◽  
High Throughput Analysis ◽  
Throughput Analysis

Nucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification. However, the requirement for different working temperatures leads to the need of a thermocycler machine or complex thermal apparatus, which have been preventing its application in novel integrated devices for single workflow and high throughput analysis. Conversely, isothermal amplification methods have been gaining attention, especially for point-of-care diagnosis and applications. These non-PCR based methods have been developed by mimicking the in vivo amplification mechanisms, while performing the amplification with high sensitivity, selectivity and allowing for high-throughput analysis. These favorable capabilities have pushed forward the implementation and commercialization of several platforms that exploit isothermal amplification methods, mostly against virus, bacteria and other pathogens in water, food, environmental and clinical samples. Nevertheless, the future of isothermal amplification methods is still dependent on achieving technical maturity and broader commercialization of enzymes and reagents.

Combination of Free Software, Light Microscopy and Non-specific Dyes for a High-throughput Analysis in Live/Dead Cell Count

2015 ◽  
Vol 11 (4) ◽  
pp. 233-238 ◽  
Author(s):  
Luciano Cardoso ◽  
Suellen Cordeiro ◽  
Marcio Fronza ◽  
Denise Endringer ◽  
Tadeu de Andrade ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Cell Count ◽  
High Throughput ◽  
Free Software ◽  
Dead Cell ◽  
High Throughput Analysis ◽  
Throughput Analysis

Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell–ECM Interactions

2021 ◽  
Author(s):  
Ruoxing Lei ◽  
Erin A. Akins ◽  
Kelly C. Y. Wong ◽  
Nicole A. Repina ◽  
Kayla J. Wolf ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis

Development of pattern recognition based on the nanosheet-DNA probes and an extendible DNA library

The Analyst ◽  
2021 ◽  
Author(s):  
Jiawei Qi ◽  
Pinhua Rao ◽  
Lele Wang ◽  
Li Xu ◽  
Yanli Wen ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
High Throughput ◽  
Analysis Data ◽  
Dna Probes ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Library ◽  
Array Sensing

Pattern recognition, also called “array sensing” is a recognition strategy with a wide and expandable analysis range, based on the high-throughput analysis data. In this work, we constructed a sensor...

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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