scholarly journals Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

10.3791/55831 ◽  
2017 ◽  
Author(s):  
Yuetian Zhang ◽  
Yi Pan ◽  
Hiroaki Matsunami ◽  
Hanyi Zhuang
Keyword(s):  
Real Time ◽  
Odorant Receptor ◽  
Receptor Activation ◽  
Live Cell ◽  
Camp Assay ◽  
Cell Measurement

scholarly journals Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

10.3791/59446 ◽  
2019 ◽  
Author(s):  
Claire A. de March ◽  
Yosuke Fukutani ◽  
Aashutosh Vihani ◽  
Hitoshi Kida ◽  
Hiroaki Matsunami
Keyword(s):  
Real Time ◽  
Vapor Phase ◽  
Odorant Receptor ◽  
Receptor Activation

A novel microfluidic microelectrode chip for a significantly enhanced monitoring of NPY-receptor activation in live mode

Lab on a Chip ◽  
2017 ◽  
Vol 17 (24) ◽  
pp. 4294-4302 ◽  
Author(s):  
Franziska D. Zitzmann ◽  
Heinz-Georg Jahnke ◽  
Felix Nitschke ◽  
Annette G. Beck-Sickinger ◽  
Bernd Abel ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Chip ◽  
Microelectrode Array ◽  
Fem Simulation ◽  
Receptor Activation ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Non Invasive ◽  
Npy Receptor ◽  
Simulation Based

We present a FEM simulation based step-by-step development of a microelectrode array integrated into a microfluidic chip for the non-invasive real-time monitoring of living cells.

Abstract 2156: Quantifying cell subsets and heterogeneity in living cultures using real time live-cell analysis

2019 ◽  
Author(s):  
Nicola Bevan ◽  
Tim Jackson ◽  
Clare Szybut ◽  
Lauren Kelsey ◽  
Hinnah Campwala ◽  
...  
Keyword(s):  
Real Time ◽  
Live Cell ◽  
Cell Analysis ◽  
Live Cell Analysis

Abstract 2156: Quantifying cell subsets and heterogeneity in living cultures using real time live-cell analysis

2019 ◽  
Author(s):  
Nicola Bevan ◽  
Tim Jackson ◽  
Clare Szybut ◽  
Lauren Kelsey ◽  
Hinnah Campwala ◽  
...  
Keyword(s):  
Real Time ◽  
Live Cell ◽  
Cell Analysis ◽  
Live Cell Analysis

scholarly journals A Multiplexed Fluorescent Assay for Independent Second-Messenger Systems

2013 ◽  
Vol 18 (7) ◽  
pp. 797-806 ◽  
Author(s):  
Paul H. Tewson ◽  
Anne Marie Quinn ◽  
Thomas E. Hughes
Keyword(s):  
Real Time ◽  
Second Messengers ◽  
Basic Research ◽  
Second Messenger ◽  
Cell Types ◽  
Live Cell ◽  
Multiple Components ◽  
Second Messenger Systems ◽  
G Protein Coupled ◽  
Cell Signaling Pathways

There is a growing need in drug discovery and basic research to measure multiple second-messenger components of cell signaling pathways in real time and in relevant tissues and cell types. Many G-protein–coupled receptors activate the heterotrimeric protein, Gq, which in turn activates phospholipase C (PLC). PLC cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) to produce two second messengers: diacylglycerol (DAG), which remains in the plasma membrane, and inositol triphosphate (IP3), which diffuses through the cytosol to release stores of intracellular calcium ions (Ca2+). Our goal was to create a series of multiplex sensors that would make it possible to simultaneously measure two different components of the Gq pathway in living cells. Here we describe new fluorescent sensors for DAG and PIP2 that produce robust changes in green or red fluorescence and can be combined with one another, or with existing Ca2+ sensors, in a live-cell assay. These assays can detect multiple components of Gq signaling, simultaneously in real time, on standard fluorescent plate readers or live-cell imaging systems.

Real-time and quantitative fluorescent live-cell imaging with quadruplex-specific red-edge probe (G4-REP)

2017 ◽  
Vol 1861 (5) ◽  
pp. 1312-1320 ◽  
Author(s):  
Sunny Y. Yang ◽  
Souheila Amor ◽  
Aurélien Laguerre ◽  
Judy M.Y. Wong ◽  
David Monchaud
Keyword(s):  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Red Edge ◽  
Edge Probe

Single live cell imaging for real-time monitoring of resistance mechanism in Pseudomonas aeruginosa

2002 ◽  
Vol 7 (4) ◽  
pp. 576 ◽  
Author(s):  
Sophia V. Kyriacou ◽  
Michelle E. Nowak ◽  
William J. Brownlow ◽  
Xiao-Hong Nancy Xu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Resistance Mechanism ◽  
Live Cell ◽  
Real Time Monitoring
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