scholarly journals Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

10.3791/55627 ◽  
2017 ◽  
Author(s):  
Ben Katz ◽  
Rita Gutorov ◽  
Elisheva Rhodes-Mordov ◽  
Roger C. Hardie ◽  
Baruch Minke
Keyword(s):  
Voltage Clamp ◽  
Cell Voltage ◽  
Whole Cell ◽  
Whole Cell Voltage Clamp ◽  
Electrophysiological Method

Developmental change in fast Na channel properties in embryonic chick ventricular heart cells

1995 ◽  
Vol 73 (10) ◽  
pp. 1475-1484 ◽  
Author(s):  
Hideaki Sada ◽  
Takashi Ban ◽  
Takeshi Fujita ◽  
Yoshio Ebina ◽  
Nicholas Sperelakis
Keyword(s):  
Steady State ◽  
Voltage Clamp ◽  
Time Constant ◽  
Voltage Dependence ◽  
Cell Voltage ◽  
Developmental Changes ◽  
Heart Cells ◽  
Embryonic Chick ◽  
Whole Cell ◽  
Whole Cell Voltage Clamp

To assess developmental changes in kinetic properties of the cardiac sodium current, whole-cell voltage-clamp experiments were conducted using 3-, 10-, and 17-day-old embryonic chick ventricular heart cells. Experimental data were quantified according to the Hodgkin–Huxley model. While the Na current density, as examined by the maximal conductance, drastically increased (six- to seven-fold) with development, other current–voltage parameters remained unchanged. Whereas the activation time constant and the steady-state activation characteristics were comparable among the three age groups, the voltage dependence of the inactivation time constant and the steady-state inactivation underwent a shift in the voltage dependence toward negative potentials during embryonic development. Consequently, the steady-state (window current) conductance, which was sufficient to induce automatic activity in the young embryos, was progressively reduced with age.Key words: cardiac electrophysiology, whole-cell voltage-clamp experiments, fast Na currents, heart, development, developmental changes.

A-current in rat globus pallidus: A whole-cell voltage clamp study on acutely dissociated neurons

1992 ◽  
Vol 144 (1-2) ◽  
pp. 4-8 ◽  
Author(s):  
Alessandro Stefani ◽  
Paolo Calabresi ◽  
Nicola B. Mercuri ◽  
Giorgio Bernardi
Keyword(s):  
Voltage Clamp ◽  
Globus Pallidus ◽  
Cell Voltage ◽  
Whole Cell ◽  
Whole Cell Voltage Clamp ◽  
Clamp Study ◽  
A Current

Stimulation of Ca2+ current by phorbol esters in rat myometrial cells is dependent on intracellular Ca2+ concentration

1996 ◽  
Vol 8 (8) ◽  
pp. 1147 ◽  
Author(s):  
M Kusaka ◽  
N Sperelakis
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Voltage Clamp ◽  
Phorbol Esters ◽  
Cell Voltage ◽  
Muscle Cells ◽  
Whole Cell ◽  
Perforated Patch ◽  
Kinase C ◽  
Whole Cell Voltage Clamp

The effects of phorbol esters on the L-type Ca2+ current (ICa(L)) were investigated using nystatin-perforated patch and standard whole-cell voltage clamp in uterine smooth muscle cells isolated from late-pregnant rats. Using nystatin-perforated patch to maintain the integrity of the cytosol components, phorbol 12-myristate 13-acetate (PMA, 300 nM) increased ICa(L). When the standard whole-cell voltage clamp was used, the effect of PMA was dependent on the Ca2+ concentration in the pipette solution: PMA enhanced ICa(L) at pCa 6 and pCa 7 but not at pCa 10 or pCa 8. The effect of PMA was reversed by a selective inhibitor of protein kinase C, calphostin-C (500 nM). It is concluded that phorbol esters stimulate ICa(L) in uterine muscle cells and that the isoform of protein kinase C involved in this effect is Ca2+ dependent. This mechanism may be involved in the regulation of uterine contraction during pregnancy.

scholarly journals Whole-cell Voltage Clamp Measurements of Anthrax Toxin Pore Current

2005 ◽  
Vol 280 (47) ◽  
pp. 39417-39422 ◽  
Author(s):  
Joshua T. Wolfe ◽  
Bryan A. Krantz ◽  
G. Jonah A. Rainey ◽  
John A. T. Young ◽  
R. John Collier
Keyword(s):  
Voltage Clamp ◽  
Cell Voltage ◽  
Anthrax Toxin ◽  
Whole Cell ◽  
Whole Cell Voltage Clamp

An iconographic program for computer-controlled whole-cell voltage clamp experiments

1993 ◽  
Vol 48 (1-2) ◽  
pp. 65-74 ◽  
Author(s):  
Dénes Budai ◽  
Lois J. Kehl ◽  
Georgetta I. Poliac ◽  
George L. Wilcox
Keyword(s):  
Voltage Clamp ◽  
Cell Voltage ◽  
Whole Cell ◽  
Whole Cell Voltage Clamp ◽  
Computer Controlled

Protein kinase C stimulates Ca2+ current in pregnant rat myometrial cells

1994 ◽  
Vol 72 (11) ◽  
pp. 1304-1307 ◽  
Author(s):  
Keiichi Shimamura ◽  
Masumi Kusaka ◽  
Nicholas Sperelakis
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Voltage Clamp ◽  
Cell Voltage ◽  
Pregnant Rat ◽  
Whole Cell ◽  
Myometrial Cells ◽  
Kinase C ◽  
Whole Cell Voltage Clamp ◽  
Voltage Dependent

The factors that regulate the voltage-dependent Ca2+ channels in pregnant uterine smooth muscle cells have not been elucidated, including any roles for protein kinase C (PKC). Therefore, the role of PKC in the regulation of the slow (L type) Ca2+ channels was examined in myometrial cells isolated from late pregnant (18–19 day) rat uterus, using the nystatin-perforated whole-cell voltage clamp. A PKC activator, phorbol 12, 13-dibutyrate (PDB), increased the L-type Ca2+ current (ICa(L)). Bath application of PDB (0.03 and 0.3 μM) increased the peak amplitude of ICa(L) by 21 ± 14% (n = 6) and 37 ± 8% (n = 9, p < 0.01), respectively. PDB did not change the holding current or shift the current–voltage relationship for ICa(L). The PKC inhibitors, H-7 (20 μM) or staurosporine (10 nM), reversed the effect of PDB. These results indicate that PKC may play a role in regulating Ca2+ channel function in pregnant rat myometrial cells and, therefore, may be involved in control of uterine contraction.Key words: protein kinase C, phorbol ester, calcium current, myometrial cell, nystatin-perforated patch, whole-cell voltage clamp.

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