scholarly journals Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

10.3791/55498 ◽  
2017 ◽  
Author(s):  
Mercedes Pardo ◽  
Daniel Bode ◽  
Lu Yu ◽  
Jyoti S. Choudhary
Keyword(s):  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

scholarly journals Bacterial protein complexes investigation using blue native PAGE

2011 ◽  
Vol 166 (1) ◽  
pp. 47-62 ◽  
Author(s):  
Jiri Dresler ◽  
Jana Klimentova ◽  
Jiri Stulik
Keyword(s):  
Protein Complexes ◽  
Bacterial Protein ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

Protein complexes of the plant plasma membrane resolved by Blue Native PAGE

2004 ◽  
Vol 121 (4) ◽  
pp. 546-555 ◽  
Author(s):  
Jonas Kjell ◽  
Allan G. Rasmusson ◽  
Hakan Larsson ◽  
Susanne Widell
Keyword(s):  
Plasma Membrane ◽  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native ◽  
Plant Plasma Membrane

Blue-Native Page in Studying Protein Complexes

2008 ◽  
pp. 557-569
Author(s):  
Holger Eubel ◽  
A. Harvey Millar
Keyword(s):  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Blue Native

Analysis of Membrane Protein Complexes by Blue Native PAGE

PROTEOMICS ◽  
2006 ◽  
Vol 6 (S2) ◽  
pp. 6-15 ◽  
Author(s):  
Veronika Reisinger ◽  
Lutz Andreas Eichacker
Keyword(s):  
Membrane Protein ◽  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Membrane Protein Complexes ◽  
Blue Native

Analysis of membrane-protein complexes of the marine sulfate reducer Desulfobacula toluolica Tol2 by 1D blue native-PAGE complexome profiling and 2D blue native-/SDS-PAGE

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 973-988 ◽  
Author(s):  
Lars Wöhlbrand ◽  
Hanna S. Ruppersberg ◽  
Christoph Feenders ◽  
Bernd Blasius ◽  
Hans-Peter Braun ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Complexes ◽  
Sulfate Reducer ◽  
Native Page ◽  
Blue Native Page ◽  
Sds Page ◽  
Membrane Protein Complexes ◽  
Blue Native ◽  
Desulfobacula Toluolica

scholarly journals The GPI Transamidase Complex of Saccharomyces cerevisiae Contains Gaa1p, Gpi8p, and Gpi16p

2001 ◽  
Vol 12 (10) ◽  
pp. 3295-3306 ◽  
Author(s):  
Patrick Fraering ◽  
Isabella Imhof ◽  
Urs Meyer ◽  
Jean-Marc Strub ◽  
Alain van Dorsselaer ◽  
...  
Keyword(s):  
Transmembrane Domain ◽  
Protein Complexes ◽  
Native Page ◽  
Transmembrane Glycoprotein ◽  
Blue Native Page ◽  
Essential Components ◽  
Precursor Proteins ◽  
Gpi Proteins ◽  
Gpi Transamidase ◽  
Blue Native

Gpi8p and Gaa1p are essential components of the GPI transamidase that adds glycosylphosphatidylinositols (GPIs) to newly synthesized proteins. After solubilization in 1.5% digitonin and separation by blue native PAGE, Gpi8p is found in 430–650-kDa protein complexes. These complexes can be affinity purified and are shown to consist of Gaa1p, Gpi8p, and Gpi16p (YHR188c). Gpi16p is an essential N-glycosylated transmembrane glycoprotein. Its bulk resides on the lumenal side of the ER, and it has a single C-terminal transmembrane domain and a small C-terminal, cytosolic extension with an ER retrieval motif. Depletion of Gpi16p results in the accumulation of the complete GPI lipid CP2 and of unprocessed GPI precursor proteins. Gpi8p and Gpi16p are unstable if either of them is removed by depletion. Similarly, when Gpi8p is overexpressed, it largely remains outside the 430–650-kDa transamidase complex and is unstable. Overexpression of Gpi8p cannot compensate for the lack of Gpi16p. Homologues of Gpi16p are found in all eucaryotes. The transamidase complex is not associated with the Sec61p complex and oligosaccharyltransferase complex required for ER insertion and N-glycosylation of GPI proteins, respectively. When GPI precursor proteins or GPI lipids are depleted, the transamidase complex remains intact.

Blue native-PAGE analysis of membrane protein complexes in Porphyromonas gingivalis

2014 ◽  
Vol 110 ◽  
pp. 72-92 ◽  
Author(s):  
Michelle D. Glew ◽  
Paul D. Veith ◽  
Dina Chen ◽  
Christine A. Seers ◽  
Yu-Yen Chen ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Porphyromonas Gingivalis ◽  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Membrane Protein Complexes ◽  
Blue Native

scholarly journals A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

2011 ◽  
Vol 11 (1) ◽  
pp. 22 ◽  
Author(s):  
Yanfeng Peng ◽  
Yuanming Luo ◽  
Tingting Yu ◽  
Xinping Xu ◽  
Keqiang Fan ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Clostridium Thermocellum ◽  
Protein Complexes ◽  
Native Page ◽  
Blue Native Page ◽  
Membrane Protein Complexes ◽  
Blue Native
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