scholarly journals Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

10.3791/55489 ◽  
2017 ◽  
Author(s):  
Qing-Wei Huo ◽  
Sidra Tabassum ◽  
Afzal Ali Misrani ◽  
Cheng Long
Keyword(s):  
Neonatal Rats ◽  
Novel Method ◽  
Freely Moving

A simple method for implanting electrodes in freely moving neonatal rats

1982 ◽  
Vol 6 (1-2) ◽  
pp. 175-177 ◽  
Author(s):  
Enrico Cherubini ◽  
Maria Rita de Feo ◽  
Oriano Mecarelli ◽  
Gianfranco Ricci
Keyword(s):  
Neonatal Rats ◽  
Simple Method ◽  
Freely Moving

μ-1 Opioid Receptor Stimulation Decreases Body Temperature in Conscious, Unrestrained Neonatal Rats

2002 ◽  
Vol 227 (6) ◽  
pp. 377-381 ◽  
Author(s):  
Atalie S. Colman ◽  
John H. Miller
Keyword(s):  
Body Temperature ◽  
Age Groups ◽  
Neonatal Rats ◽  
Experimental Conditions ◽  
Rat Pups ◽  
Opioid Agonists ◽  
Freely Moving ◽  
Induced Changes ◽  
Conscious Animals ◽  
Stimulation Of

The influence of μ-selective opioid agonists on neonatal thermoregulatory mechanisms has received little attention. Opioid treatment in adult subjects can cause either hyper- or hypothermia, depending on the experimental conditions, the strain of rat used, and the dose and route of administration of the drug. The present study assessed the effect of two μ opioid agonists on body temperature in neonatal Wistar rats aged 2 to 13 days. Rat pups were administered either saline or one of the two μ-selective opioid agonists, dermorphin (0.4 mg/kg) or fentanyl (0.06 mg/kg), by subcutaneous injection. Continuous rectal temperatures were measured both prior to and following drug or saline injection in freely moving, conscious animals. Ambient temperature in a plethysmograph chamber was maintained within or close to the thermoneutral zone for pups (32°C). To distinguish between μ-1 and μ-2 effects, all animals received either saline or 10 mg/kg of the irreversible μ-1 antagonist naloxonazine (NALZ) 1 day prior to agonist administration. NALZ on its own had no effect on body temperature. Dermorphin and fentanyl both caused a fall in body temperature in pups of all age groups. The temperature decreases ranged from 0.8°-2.2°C. These opioid-Induced changes were inhibited by NALZ pretreatment. Although there was no evidence for endogenous μ-1 opioid activity, this study indicated that stimulation of μ-1 opioid receptors causes a decrease in body temperature in conscious, unrestrained neonatal rats under or close to thermoneutral conditions.

scholarly journals A novel method for modeling facial allodynia associated with migraine in awake and freely moving rats

2010 ◽  
Vol 185 (2) ◽  
pp. 236-245 ◽  
Author(s):  
Julie Wieseler ◽  
Amanda Ellis ◽  
David Sprunger ◽  
Kim Brown ◽  
Andrew McFadden ◽  
...  
Keyword(s):  
Freely Moving Rats ◽  
Novel Method ◽  
Freely Moving

scholarly journals In vivo brain activity imaging of interactively locomoting mice

2017 ◽  
Author(s):  
Shigenori Inagaki ◽  
Masakazu Agetsuma ◽  
Shinya Ohara ◽  
Toshio Iijima ◽  
Tetsuichi Wazawa ◽  
...  
Keyword(s):  
Visual Cortex ◽  
Primary Visual Cortex ◽  
Brain Activity ◽  
Field Potential ◽  
Brain Functions ◽  
Wide Range ◽  
Novel Method ◽  
Freely Moving ◽  
Voltage Indicator

AbstractElectrophysiological field potential dynamics have been widely used to investigate brain functions and related psychiatric disorders. Conversely, however, various technical limitations of conventional recording methods have limited its applicability to freely moving subjects, especially when they are in a group and socially interacting with each other. Here, we propose a new method to overcome these technical limitations by introducing a bioluminescent voltage indicator called LOTUS-V. Using our simple and fiber-free recording method, named “SNIPA,” we succeeded in capturing brain activity in freely-locomotive mice, without the need for complicated instruments. This novel method further allowed us to simultaneously record from multiple independently-locomotive animals that were interacting with one another. Further, we successfully demonstrated that the primary visual cortex was activated during the interaction. This methodology will further facilitate a wide range of studies in neurobiology and psychiatry.

scholarly journals A novel method of sequential urine collection and recording in freely moving mice

2009 ◽  
Vol 133 (6) ◽  
pp. 332-336 ◽  
Author(s):  
Gen Yamamoto ◽  
Nahoko Kai ◽  
Tetsuya Shirasaki ◽  
Fumio Soeda ◽  
Kazuo Takahama
Keyword(s):  
Urine Collection ◽  
Novel Method ◽  
Freely Moving
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