scholarly journals Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

10.3791/55122 ◽  
2017 ◽  
Author(s):  
Dmitry V. Samigullin ◽  
Eduard F. Khaziev ◽  
Nikita V. Zhilyakov ◽  
Ellya A. Bukharaeva ◽  
Eugeny E. Nikolsky
Keyword(s):  
Neuromuscular Junction ◽  
Calcium Transient ◽  
Nerve Endings ◽  
Frog Neuromuscular Junction ◽  
Nerve Stump

scholarly journals Voltage-Dependent P/Q-Type Calcium Channels at the Frog Neuromuscular Junction

2011 ◽  
pp. 815-823 ◽  
Author(s):  
L. F. NURULLIN ◽  
A. R. MUKHITOV ◽  
A. N. TSENTSEVYTSKY ◽  
N. V. PETROVA ◽  
D. V. SAMIGULLIN ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Calcium Channels ◽  
Motor Nerve ◽  
Calcium Transient ◽  
Functional Expression ◽  
Frog Neuromuscular Junction ◽  
Motor Nerve Ending ◽  
Voltage Gated ◽  
Voltage Gated Calcium Channels ◽  
Release Of Acetylcholine

It is well known that antagonists of N-type voltage-gated calcium channels inhibit the evoked quantal release of acetylcholine in amphibian neuromuscular synapses. This, however, does not exclude the functional expression of other types of voltage-gated calcium channels in these nerve terminals. Using immunocytochemistry, we detected the expression of the 1A subunit of P/Q-type calcium channels (that is otherwise typical of mammalian motor nerve endings) in the frog neuromuscular junction. In addition, we demonstrated that the P/Q-type channel blocker ω-agatoxin IVA (20 nM) reduced the action potential-induced calcium transient and significantly decreased both spontaneous and evoked mediator release. Our data indicates the functional expression of P/Q-type calcium channels in the frog motor nerve ending which participate in acetylcholine release.

221 Presynaptic Ca2+-imaging at the frog neuromuscular junction

1996 ◽  
Vol 25 ◽  
pp. S36 ◽  
Author(s):  
M. Osanai ◽  
S. Suzuki ◽  
N. Suzuki ◽  
K. Kuba ◽  
H. Kijima
Keyword(s):  
Neuromuscular Junction ◽  
Frog Neuromuscular Junction
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