scholarly journals Murine Full-thickness Skin Transplantation

10.3791/55105 ◽  
2017 ◽  
Author(s):  
Chih–Hsien Cheng ◽  
Chen–Fang Lee ◽  
Madeline Fryer ◽  
Georg J. Furtmüller ◽  
Byoungchol Oh ◽  
...  
Keyword(s):  
Skin Transplantation ◽  
Thickness Skin

Continuous Aspirin Use Does Not Increase Bleeding Risk of Split-Thickness Skin Transplantation Repair to Chronic Wounds

2017 ◽  
Vol 21 (4) ◽  
pp. 316-319
Author(s):  
Yanwei Sun ◽  
Yibing Wang ◽  
Liang Li ◽  
Zheng Zhang ◽  
Ning Wang ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Odds Ratio ◽  
Chronic Wounds ◽  
Univariate Analysis ◽  
Bleeding Risk ◽  
Bleeding Complications ◽  
Skin Transplantation ◽  
Control Groups ◽  
Thickness Skin ◽  
And Control

Background: Discontinuation of aspirin therapy before cutaneous surgery may cause serious complications. Objectives: The aim of this prospective study was to evaluate the bleeding risk of split-thickness skin transplantation repair to chronic wounds in patients on aspirin therapy. Methods: A total of 97 patients who underwent split-thickness skin transplantation surgery of chronic wounds during a 2-year period were enrolled. They were categorized on the basis of aspirin therapies. The primary outcome was postoperative bleeding and bleeding complications. Univariate analysis was performed to examine the association between aspirin and bleeding complications. Among the 26 patients taking aspirin continuously in group A, there were 5 bleeding complications (19.23%). Among the 55 nonusers in group B, there were 10 bleeding complications (18.18%). Among the 16 discontinuous patients in group C, there were 3 bleeding complications (18.75%). No statistical differences were found among the groups ( P = .956). Univariate analysis showed that continuous aspirin use was not significantly associated with bleeding complications (odds ratio, 0.933; 95% confidence interval, 0.283-3.074; P = .910 in the aspirin and control groups) and that discontinuous aspirin use was not significantly associated with bleeding complications (odds ratio, 0.963; 95% confidence interval, 0.230-4.025; P = .959 in the aspirin and control groups; odds ratio, 0.969; 95% confidence interval, 0.198-4.752; P = .969 in the aspirin and discontinuous groups). Conclusions: Continuous aspirin use does not produce an additional bleeding risk in patients who undergo split-thickness skin transplantation repair of chronic wounds.

miR-31, miR-155, and miR-221 expression profiles and their association with graft skin tolerance in a syngeneic vs. allogeneic murine skin transplantation model

2022 ◽  
Author(s):  
Edna Ayerim Mandujano-Tinoco ◽  
Francisco González-García ◽  
Rosa M Salgado ◽  
René Fernando Abarca-Buis ◽  
José Manuel Sanchez-Lopez ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Structural Characteristics ◽  
Expression Profiles ◽  
Mouse Strains ◽  
Skin Transplantation ◽  
Post Transplantation ◽  
Thickness Skin ◽  
Significant Difference ◽  
Tgf Β1 ◽  
Transplantation Model

Abstract Grafting is the gold standard for the treatment of severe skin burns. Frequently, allogeneic tissue is the only transient option for wound coverage, but their use risks damage to surrounding tissues. MicroRNAs have been associated with acute rejection of different tissues/organs. In this study, we analyzed the expression of miR-31, miR-155, and miR-221 and associate it with graft tolerance or rejection using a murine full-thickness skin transplantation model. Recipient animals for the syngeneic and allogeneic groups were BALB/c and C57BL/6 mice, respectively; donor tissues were obtained from BALB/c mice. After 7 days post-transplantation (DPT), the recipient skin and grafts in the syngeneic group maintained most of their structural characteristics and transforming growth factor (TGF)β1 and TGFβ3 expression. Allografts were rejected early (Banff grades II and IV at 3 and 7 DPT, respectively), showing damage to the skin architecture and alteration of TGFβ3 distribution. miRNAs skin expression changed in both mouse strains; miR-31 expression increased in the recipient skin of syngeneic grafts relative to that of allogeneic grafts at 3 and 7 DPT (p < 0.05 and p < 0.01, respectively); miR-221 expression increased in the same grafts at 7 DPT (p < 0.05). The only significant difference between donor tissues was observed for miR-155 expression at 7 DPT which was associated with necrotic tissue. Only miR-31 and miR-221 levels were increased in the blood of BALB/c mice that received syngeneic grafts after 7 DPT. Our data suggest that local and systemic miR-31 and miR-221 overexpression are associated with graft tolerance.

Outcomes for split-thickness skin transplantation in high-risk patients using octenidine

2015 ◽  
Vol 24 (Sup6) ◽  
pp. S8-S12 ◽  
Author(s):  
J. Matiasek ◽  
G. Djedovic ◽  
L. Unger ◽  
H. Beck ◽  
M. Mattesich ◽  
...  
Keyword(s):  
High Risk ◽  
Skin Transplantation ◽  
High Risk Patients ◽  
Thickness Skin ◽  
Risk Patients

Microvascular integrity plays a role in graft survival after skin transplantation

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
C. Heim ◽  
B. Motsch ◽  
N. Koch ◽  
M. Ramsperger-Gleixner ◽  
M. Weyand ◽  
...  
Keyword(s):  
Graft Survival ◽  
Skin Transplantation
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