scholarly journals Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

10.3791/54042 ◽  
2016 ◽  
Author(s):  
Allison S. Harney ◽  
Yarong Wang ◽  
John S. Condeelis ◽  
David Entenberg
Keyword(s):  
Tumor Microenvironment ◽  
Real Time ◽  
Time Lapse ◽  
Intravital Imaging ◽  
Extended Time

scholarly journals Near-surface real-time seismic imaging using parsimonious interferometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sherif M. Hanafy ◽  
Hussein Hoteit ◽  
Jing Li ◽  
Gerard T. Schuster
Keyword(s):  
Fluid Flow ◽  
Real Time ◽  
Temporal Resolution ◽  
Seismic Imaging ◽  
Time Lapse ◽  
Rock Properties ◽  
Recording Time ◽  
Near Surface ◽  
Arrival Times ◽  
Order Of Magnitude

AbstractResults are presented for real-time seismic imaging of subsurface fluid flow by parsimonious refraction and surface-wave interferometry. Each subsurface velocity image inverted from time-lapse seismic data only requires several minutes of recording time, which is less than the time-scale of the fluid-induced changes in the rock properties. In this sense this is real-time imaging. The images are P-velocity tomograms inverted from the first-arrival times and the S-velocity tomograms inverted from dispersion curves. Compared to conventional seismic imaging, parsimonious interferometry reduces the recording time and increases the temporal resolution of time-lapse seismic images by more than an order-of-magnitude. In our seismic experiment, we recorded 90 sparse data sets over 4.5 h while injecting 12-tons of water into a sand dune. Results show that the percolation of water is mostly along layered boundaries down to a depth of a few meters, which is consistent with our 3D computational fluid flow simulations and laboratory experiments. The significance of parsimonious interferometry is that it provides more than an order-of-magnitude increase of temporal resolution in time-lapse seismic imaging. We believe that real-time seismic imaging will have important applications for non-destructive characterization in environmental, biomedical, and subsurface imaging.

scholarly journals Module to Support Real-Time Microscopic Imaging of Living Organisms on Ground-Based Microgravity Analogs

2021 ◽  
Vol 11 (7) ◽  
pp. 3122
Author(s):  
Srujana Neelam ◽  
Audrey Lee ◽  
Michael A. Lane ◽  
Ceasar Udave ◽  
Howard G. Levine ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Simulated Microgravity ◽  
Image Acquisition ◽  
Time Lapse ◽  
Moving Frames ◽  
Cell Functions ◽  
Microgravity Simulation ◽  
Division Cell ◽  
Over Time

Since opportunities for spaceflight experiments are scarce, ground-based microgravity simulation devices (MSDs) offer accessible and economical alternatives for gravitational biology studies. Among the MSDs, the random positioning machine (RPM) provides simulated microgravity conditions on the ground by randomizing rotating biological samples in two axes to distribute the Earth’s gravity vector in all directions over time. Real-time microscopy and image acquisition during microgravity simulation are of particular interest to enable the study of how basic cell functions, such as division, migration, and proliferation, progress under altered gravity conditions. However, these capabilities have been difficult to implement due to the constantly moving frames of the RPM as well as mechanical noise. Therefore, we developed an image acquisition module that can be mounted on an RPM to capture live images over time while the specimen is in the simulated microgravity (SMG) environment. This module integrates a digital microscope with a magnification range of 20× to 700×, a high-speed data transmission adaptor for the wireless streaming of time-lapse images, and a backlight illuminator to view the sample under brightfield and darkfield modes. With this module, we successfully demonstrated the real-time imaging of human cells cultured on an RPM in brightfield, lasting up to 80 h, and also visualized them in green fluorescent channel. This module was successful in monitoring cell morphology and in quantifying the rate of cell division, cell migration, and wound healing in SMG. It can be easily modified to study the response of other biological specimens to SMG.

Collecting Vehicle-Speed Data by Using Time-Lapse Video Recording Equipment

2003 ◽  
Vol 1855 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Christopher Strong ◽  
Scott Lowry ◽  
Peter McCarthy
Keyword(s):  
Real Time ◽  
Video Recording ◽  
Warning System ◽  
Time Lapse ◽  
Vehicle Speed ◽  
Recording Equipment ◽  
Safety Improvement ◽  
Camera Location ◽  
Message Signs ◽  
Time Lapse Video

An innovative application of time-lapse video recording is used to assist in an evaluation of a highway safety improvement. The improvement is an icy-curve warning system near Fredonyer Summit in northern California that activates real-time motorist warnings via extinguishable message signs, based on weather readings collected from road weather information systems. A measure of effectiveness is whether motorist speed is reduced as a result of real-time warnings to drivers. Why indirect speed measurement with video was preferred over radar for this case is discussed, as is how specific methodological issues related to the custom-built equipment, including camera location and orientation, distance benchmarking, and data collection and reduction. Theoretical and empirical accuracy measurements show that the video surveillance trailers yield results comparable to radar and, hence, would be applicable for studies in which speed change is measured. Because this particular technology had not been used previously, several lessons are documented that may help determine where and how similar equipment may be optimally used in future studies.

scholarly journals Ultrasound and Transcriptomics Identify a Differential Impact of Cisplatin and Histone Deacetylation on Tumor Structure and Microenvironment in a Patient-Derived In Vivo Model of Gastric Cancer

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1485
Author(s):  
Aina Venkatasamy ◽  
Eric Guerin ◽  
Anais Blanchet ◽  
Christophe Orvain ◽  
Véronique Devignot ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Microenvironment ◽  
Real Time ◽  
Histone Deacetylase Inhibitors ◽  
Structural Changes ◽  
Histone Deacetylation ◽  
In Vivo Model ◽  
Specific Cell ◽  
The Impact

The reasons behind the poor efficacy of transition metal-based chemotherapies (e.g., cisplatin) or targeted therapies (e.g., histone deacetylase inhibitors, HDACi) on gastric cancer (GC) remain elusive and recent studies suggested that the tumor microenvironment could contribute to the resistance. Hence, our objective was to gain information on the impact of cisplatin and the pan-HDACi SAHA (suberanilohydroxamic acid) on the tumor substructure and microenvironment of GC, by establishing patient-derived xenografts of GC and a combination of ultrasound, immunohistochemistry, and transcriptomics to analyze. The tumors responded partially to SAHA and cisplatin. An ultrasound gave more accurate tumor measures than a caliper. Importantly, an ultrasound allowed a noninvasive real-time access to the tumor substructure, showing differences between cisplatin and SAHA. These differences were confirmed by immunohistochemistry and transcriptomic analyses of the tumor microenvironment, identifying specific cell type signatures and transcription factor activation. For instance, cisplatin induced an “epithelial cell like” signature while SAHA favored a “mesenchymal cell like” one. Altogether, an ultrasound allowed a precise follow-up of the tumor progression while enabling a noninvasive real-time access to the tumor substructure. Combined with transcriptomics, our results underline the different intra-tumoral structural changes caused by both drugs that impact differently on the tumor microenvironment.

scholarly journals Antibiotic action revealed by real-time imaging of the mycobacterial membrane

2022 ◽  
Author(s):  
Michael G. Wuo ◽  
Charles L Dulberger ◽  
Robert A. Brown ◽  
Alexander Sturm ◽  
Eveline Ultee ◽  
...  
Keyword(s):  
Real Time ◽  
Immune Modulation ◽  
Cell Envelope ◽  
Membrane Vesicles ◽  
Time Lapse ◽  
Outer Membrane Vesicles ◽  
Imaging Data ◽  
Minimal Cell ◽  
Mycobacterial Cell ◽  
Cellular Phenotypes

The current understanding of mycobacterial cell envelope remodeling in response to antibiotics is limited. Chemical tools that report on phenotypic changes with minimal cell wall perturbation are critical to gaining insight into this time-dependent phenomenon. Herein we describe a fluorogenic chemical probe that reports on mycobacterial cell envelope assembly in real time. We used time-lapse microscopy to reveal distinct spatial and temporal changes in the mycobacterial membrane upon treatment with frontline antibiotics. Differential antibiotic treatment elicited unique cellular phenotypes, providing a platform for monitoring cell envelope construction and remodeling responses simultaneously. Analysis of the imaging data indicates a role for antibiotic-derived outer membrane vesicles in immune modulation.

scholarly journals Impact of Platelet Count on Bleeding in the Setting of Anti-Platelet Therapy

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 18-18
Author(s):  
Robert Hugh Lee ◽  
Wolfgang Bergmeier
Keyword(s):  
Real Time ◽  
Conflicts Of Interest ◽  
Thrombotic Event ◽  
Platelet Inhibition ◽  
Intravital Imaging ◽  
Severe Thrombocytopenia ◽  
Platelet Counts ◽  
Anti Platelet Therapy ◽  
The Impact

Anti-platelet therapy (APT) is used for secondary prevention of thrombosis. The most commonly prescribed anti-platelet drugs are aspirin and P2Y12 inhibitors, including clopidogrel, prasugrel and ticagrelor. Dual anti-platelet therapy (DAPT) consisting of aspirin and a P2Y12 inhibitor is often used in the first 1-12 months after an initial thrombotic event and has a greater anti-thrombotic effect than single agents, but is also associated with a higher risk of bleeding. Due to this risk of hemorrhage, the appropriate use of DAPT in patients requiring percutaneous coronary intervention (PCI) with baseline or periprocedural thrombocytopenia remains unclear. To study the impact of thrombocytopenia on bleeding with APT, we used intravital imaging in a murine hemostasis model and adoptive platelet transfer to generate mice with specific platelet counts with or without platelet inhibition. To generate experimental mice, we used transgenic mice in which platelets express a chimeric GPIb receptor with the extracellular domain replaced with a domain of the human IL-4R (hIL-4R/GPIb-Tg). Endogenous platelets were depleted by injection of anti-hIL-4R antibody, and the recipient mice were then transfused with wild-type (WT) platelets from donor mice treated, or not, with single or dual APT (aspirin 20 mg/kg; clopidogrel 25 mg/kg) to achieve specific platelet counts ranging from 50,000 to 400,000 platelets/μL. We also compared these mice with WT mice (with normal platelet counts, ~1,200,000 platelets/μL) treated with APT. Platelet inhibition was confirmed prior to performing in vivo experiments. Hemostasis was determined by intravital imaging in our saphenous vein laser injury model, in which a 50 μm injury was induced by laser ablation. Real-time top-down epifluorescence imaging was used to determine time to initial hemostasis, rebleeding events, and platelet and fibrin accumulation. In each mouse, 3-5 injuries were induced at different sites and each injury was visualized for 10 minutes. Following real-time imaging, spinning disk confocal Z-stacks of platelet plugs were obtained for 3D reconstruction to compare platelet plug volume. In untreated WT mice, hemostasis was achieved in ~20 seconds. In WT mice treated with DAPT, initial hemostasis was often rapidly achieved but this was followed by significant rebleeding events. Paradoxically, platelet accumulation was increased in WT + DAPT mice due to extravascular accumulation of platelets which occurred during bleeding. However, in plugs that stabilized, plug volume was reduced in WT + DAPT mice. In hIL-4R/GPIb-Tg mice with reduced platelet counts, untreated platelets were able to form a stable hemostatic plug even at 50,000/μL, although time to hemostasis was slightly prolonged. However, as platelet counts decreased in mice with DAPT-treated platelets, initial hemostasis became more prolonged and many injuries never achieved initial hemostasis. These results suggest that DAPT may not be safe in the setting of severe thrombocytopenia. Disclosures No relevant conflicts of interest to declare.

Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment

2019 ◽  
Vol 167 (1) ◽  
pp. 123-130 ◽  
Author(s):  
S. S. Vodopyanov ◽  
M. A. Kunin ◽  
A. S. Garanina ◽  
N. F. Grinenko ◽  
K. Yu. Vlasova ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Fluorescent Proteins ◽  
Intravital Imaging

Synthetic versus real time‐lapse seismic data at the Sleipner CO2 injection site

2007 ◽  
Author(s):  
R. J. Arts ◽  
R. A. Chadwick ◽  
O. Eiken ◽  
M. Trani ◽  
S. Dortland
Keyword(s):  
Injection Site ◽  
Real Time ◽  
Seismic Data ◽  
Time Lapse ◽  
Co2 Injection

Real-Time Video Acquisition and Frame Compression Processing Technology Based on FFmpeg

2014 ◽  
Vol 631-632 ◽  
pp. 494-497 ◽  
Author(s):  
Yun Geng Xu ◽  
San Xing Cao
Keyword(s):  
Real Time ◽  
Time Lapse ◽  
Digital Transformation ◽  
Processing Technology ◽  
Broad Application ◽  
Video Information ◽  
Video Acquisition ◽  
Time Lapse Photography

Currently, video has become the most popular form of media, traditional video field is facing simulated to digital transformation. Using the computer to process the video information has a broad application prospect in many areas. This paper proposes an approach of real-time video acquisition and frame compression processing technology by using the FFmpeg and PHP function to achieve the effect of time-lapse photography. Finally, through the Apache log test the system running status.

Sign in / Sign up

Export Citation Format

Share Document