scholarly journals Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

10.3791/53186 ◽  
2015 ◽  
Author(s):  
Diana B. Peckys ◽  
Niels de Jonge
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Correlative Microscopy ◽  
Intact Cells ◽  
Epidermal Growth

scholarly journals Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor

1989 ◽  
Vol 262 (2) ◽  
pp. 659-663 ◽  
Author(s):  
J J Hsuan ◽  
N Totty ◽  
M D Waterfield
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tryptic Peptide ◽  
Growth Factor Receptor ◽  
Intact Cells ◽  
C Terminus ◽  
Partial Digestion ◽  
Epidermal Growth ◽  
Autophosphorylation Site

Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.

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