scholarly journals Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

10.3791/52235 ◽  
2015 ◽  
Author(s):  
Petar N. Grozdanov ◽  
Clinton C. MacDonald
Keyword(s):  
Elongation Factor ◽  
Gibson Assembly ◽  
Plasmid Vectors ◽  
Elongation Factor 1 ◽  
Human Elongation Factor

The Human Elongation Factor 1 A-2 Gene (EEF1A2): Complete Sequence and Characterization of Gene Structure and Promoter Activity

Genomics ◽  
2000 ◽  
Vol 68 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Claus Bischoff ◽  
Søren Kahns ◽  
Ann Lund ◽  
Helle F. Jørgensen ◽  
Morten Præstegaard ◽  
...  
Keyword(s):  
Gene Structure ◽  
Promoter Activity ◽  
Elongation Factor ◽  
Complete Sequence ◽  
Elongation Factor 1 ◽  
Human Elongation Factor

scholarly journals Different Sp1 family members differentially affect transcription from the human elongation factor 1 A-1 gene promoter

1998 ◽  
Vol 333 (3) ◽  
pp. 511-517 ◽  
Author(s):  
S⊘ren J. NIELSEN ◽  
Morten PRÆSTEGAARD ◽  
Helle F. J. ØRGENSEN ◽  
Brian F. C. CLARK
Keyword(s):  
Hela Cells ◽  
Promoter Activity ◽  
Binding Proteins ◽  
Regulatory Element ◽  
Elongation Factor ◽  
Proximal Promoter ◽  
Mobility Shift ◽  
Gc Box ◽  
Elongation Factor 1 ◽  
Human Elongation Factor

The GC box is an important transcriptional regulatory element present in the promoters of many mammalian genes. In the present study we examine the effect of known GC-box-binding proteins on the promoter of the human elongation factor 1 A-1 (hEF1A-1) gene in human HeLa cells and Drosophila SL2 cells. In HeLa cells co-transfection with the GC-box-binding protein BTEB resulted in a 4–10-fold increase in hEF1A-1 promoter activity. This stimulation was dependent on a single GC box located between positions -69 and -50 of the promoter. Little or no effect was observed of other GC-box-binding proteins including Sp1, Sp3, Sp4 and BTEB2. In SL2 cells stimulation by Sp1 and Sp3 through the single GC box of the proximal promoter led to 13-fold and 21-fold increases respectively in promoter activity. Inclusion of further upstream sequences resulted in high levels of expression when Sp1 or Sp3 was co-transfected with the reporter plasmid. In this setting Sp1 stimulated transcription by 750-fold, whereas Sp3 was even more potent, yielding a 1150-fold stimulation. Mobility-shift assays performed with the promoter-proximal GC box demonstrated the binding of Sp1, Sp3 and Sp4 to this sequence. To our knowledge, the present study represents the first comparison of all known GC-box-binding proteins on a natural promoter.

scholarly journals Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tingting Li ◽  
Weigao Yuan ◽  
Shuai Qiu ◽  
Jisen Shi
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Reference Genes ◽  
Gene Selection ◽  
Elongation Factor ◽  
Reverse Transcription Pcr ◽  
Expression Of Genes ◽  
Suitable Reference ◽  
Functional Analyses ◽  
Elongation Factor 1

AbstractThe differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.

scholarly journals Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.

1985 ◽  
Vol 260 (5) ◽  
pp. 3090-3096
Author(s):  
P Cottrelle ◽  
D Thiele ◽  
V L Price ◽  
S Memet ◽  
J Y Micouin ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1
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