scholarly journals A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

10.3791/52196 ◽  
2014 ◽  
Author(s):  
Matthew J. Farrar ◽  
Chris B. Schaffer
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord

scholarly journals In Vivo Two-Photon Imaging of Neurons and Glia in the Mouse Spinal Cord

2012 ◽  
Vol 2012 (12) ◽  
pp. pdb.prot072264-pdb.prot072264 ◽  
Author(s):  
H. Steffens ◽  
F. Nadrigny ◽  
F. Kirchhoff
Keyword(s):  
Spinal Cord ◽  
Mouse Spinal Cord ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Chronic in vivo imaging in the mouse spinal cord using an implanted chamber

2012 ◽  
Vol 9 (3) ◽  
pp. 297-302 ◽  
Author(s):  
Matthew J Farrar ◽  
Ida M Bernstein ◽  
Donald H Schlafer ◽  
Thomas A Cleland ◽  
Joseph R Fetcho ◽  
...  
Keyword(s):  
Spinal Cord ◽  
In Vivo Imaging ◽  
Mouse Spinal Cord

Nuclear magnetic resonance microimaging of mouse spinal cord in vivo

2004 ◽  
Vol 15 (3) ◽  
pp. 474-482 ◽  
Author(s):  
Jean-Marie Bonny ◽  
Manuel Gaviria ◽  
Jean-Pierre Donnat ◽  
Beatrix Jean ◽  
Alain Privat ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mouse Spinal Cord ◽  
Magnetic Resonance Microimaging ◽  
Nuclear Magnetic Resonance Microimaging ◽  
Nuclear Magnetic

In vivo characterization of colorectal and cutaneous inputs to lumbosacral dorsal horn neurons in the mouse spinal cord

Neuroscience ◽  
2016 ◽  
Vol 316 ◽  
pp. 13-25 ◽  
Author(s):  
K.E. Farrell ◽  
M.M. Rank ◽  
S. Keely ◽  
A.M. Brichta ◽  
B.A. Graham ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Mouse Spinal Cord ◽  
Dorsal Horn Neurons ◽  
In Vivo Characterization

Ventral midline cells are required for the local control of commissural axon guidance in the mouse spinal cord

Development ◽  
1999 ◽  
Vol 126 (16) ◽  
pp. 3649-3659
Author(s):  
M.P. Matise ◽  
M. Lustig ◽  
T. Sakurai ◽  
M. Grumet ◽  
A.L. Joyner
Keyword(s):  
Spinal Cord ◽  
Critical Role ◽  
Floor Plate ◽  
Posterior Commissure ◽  
Ventral Midline ◽  
Mouse Spinal Cord ◽  
Commissural Axons ◽  
Midline Cells

Specialized cells at the midline of the central nervous system have been implicated in controlling axon projections in both invertebrates and vertebrates. To address the requirement for ventral midline cells in providing cues to commissural axons in mice, we have analyzed Gli2 mouse mutants, which lack specifically the floor plate and immediately adjacent interneurons. We show that a Dbx1 enhancer drives tau-lacZ expression in a subpopulation of commissural axons and, using a reporter line generated from this construct, as well as DiI tracing, we find that commissural axons projected to the ventral midline in Gli2(−/−) embryos. Netrin1 mRNA expression was detected in Gli2(−/−) embryos and, although much weaker than in wild-type embryos, was found in a dorsally decreasing gradient. This result demonstrates that while the floor plate can serve as a source of long-range cues for C-axons in vitro, it is not required in vivo for the guidance of commissural axons to the ventral midline in the mouse spinal cord. After reaching the ventral midline, most commissural axons remained clustered in Gli2(−/−) embryos, although some were able to extend longitudinally. Interestingly, some of the longitudinally projecting axons in Gli2(−/−) embryos extended caudally and others rostrally at the ventral midline, in contrast to normal embryos in which virtually all commissural axons turn rostrally after crossing the midline. This finding indicates a critical role for ventral midline cells in regulating the rostral polarity choice made by commissural axons after they cross the midline. In addition, we provide evidence that interactions between commissural axons and floor plate cells are required to modulate the localization of Nr-CAM and TAG-1 proteins on axons at the midline. Finally, we show that the floor plate is not required for the early trajectory of motoneurons or axons of the posterior commissure, whose projections are directed away from the ventral midline in both WT and Gli2(−/−) embryos, although they are less well organized in Gli2(−/−)mutants.

Degradation of nociceptin (orphanin FQ) by mouse spinal cord synaptic membranes is triggered by endopeptidase-24.11: an in vitro and in vivo study

2002 ◽  
Vol 64 (8) ◽  
pp. 1293-1303 ◽  
Author(s):  
Chikai Sakurada ◽  
Shinobu Sakurada ◽  
Tohru Orito ◽  
Koichi Tan-No ◽  
Tsukasa Sakurada
Keyword(s):  
Spinal Cord ◽  
In Vivo Study ◽  
Synaptic Membranes ◽  
Orphanin Fq ◽  
Mouse Spinal Cord ◽  
Endopeptidase 24.11
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