scholarly journals Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

10.3791/52022 ◽  
2014 ◽  
Author(s):  
Emmanuel Bikorimana ◽  
Danica Lapid ◽  
Hyewon Choi ◽  
Richard Dahl
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation ◽  
Retroviral Infection ◽  
Murine Embryonic Stem Cell

In vitro recapitulation of the urea cycle using murine embryonic stem cell-derived in vitro liver model

Amino Acids ◽  
2013 ◽  
Vol 45 (6) ◽  
pp. 1343-1351 ◽  
Author(s):  
Miho Tamai ◽  
Mami Aoki ◽  
Akihito Nishimura ◽  
Koji Morishita ◽  
Yoh-ichi Tagawa
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Urea Cycle ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cell ◽  
Liver Model

scholarly journals Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning

2007 ◽  
Vol 8 (9) ◽  
pp. R184 ◽  
Author(s):  
Michael Doss ◽  
Shuhua Chen ◽  
Johannes Winkler ◽  
Rita Hippler-Altenburg ◽  
Margareta Odenthal ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Phenotypic Analysis ◽  
Murine Embryonic Stem Cell ◽  
Insight Into

scholarly journals Global transcriptomic analysis of murine embryonic stem cell-derived brachyury+(T) cells

2010 ◽  
Vol 15 (3) ◽  
pp. 209-228 ◽  
Author(s):  
Michael Xavier Doss ◽  
Vilas Wagh ◽  
Herbert Schulz ◽  
Meelis Kull ◽  
Raivo Kolde ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Transcriptomic Analysis ◽  
Murine Embryonic Stem Cell

scholarly journals Sorting and expansion of murine embryonic stem cell colonies using micropallet arrays

2009 ◽  
Vol 75A (2) ◽  
pp. 121-129 ◽  
Author(s):  
Hamed Shadpour ◽  
Christopher E. Sims ◽  
Randy J. Thresher ◽  
Nancy L. Allbritton
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cell

scholarly journals A Novel Purification Method of Murine Embryonic Stem Cell– and Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes by Simple Manual Dissociation

2012 ◽  
Vol 17 (5) ◽  
pp. 683-691 ◽  
Author(s):  
Tadahiro Shinozawa ◽  
Hatsue Furukawa ◽  
Eimei Sato ◽  
Kenji Takami
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Pluripotent Stem Cell ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Drug Induced ◽  
Thin Glass ◽  
Murine Embryonic Stem Cell ◽  
Induced Pluripotent

Cardiomyocytes derived from embryonic stem cells (ES-CMs) and induced pluripotent stem cells (iPS-CMs) are useful for toxicity and pharmacology screening. In the present study, we found that cardiomyocyte-rich beating cell clusters (CCs) emerged from murine embryonic stem cell (mESC)–derived beating EBs and from human-induced pluripotent stem cell (hiPSC)–derived beating EBs dissociated by gentle pipetting with a thin glass pipette. The percentage of cardiac troponin T (cTnT)–positive cells in the beating CCs obtained from mESC-derived and hiPSC-derived beating EBs was higher (81.5% and 91.6%, respectively) than in beating-undissociated EBs (13.7% and 67.1%, respectively). For mESCs, the yield of cTnT-positive cells from beating CCs was estimated to be 1.6 times higher than that of beating EBs. The bromodeoxyuridine labeling index of mouse ES-CMs and human iPS-CMs in beating CCs was 1.5- and 3.2-fold, respectively, greater than those in beating EBs. To investigate the utility of the cells in toxicity assessment, we showed that doxorubicin, a cardiotoxic drug, induced myofilament disruption in cardiomyocytes isolated by this method. This simple method enables preparation of mouse ES-CMs and human iPS-CMs with better proliferative activity than beating EBs not dissociated by pipetting, and the cardiomyocytes are useful for drug-induced myocardial toxicity testing.

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