scholarly journals Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

10.3791/51653 ◽  
2014 ◽  
Author(s):  
Ilia Fishbein ◽  
Scott P. Forbes ◽  
Richard F. Adamo ◽  
Michael Chorny ◽  
Robert J. Levy ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adenoviral Vectors ◽  
Metallic Stent ◽  
Vascular Gene Transfer

scholarly journals Efficient and Selective Gene Transfer into Primary Human Brain Tumors by Using Single-Chain Antibody-Targeted Adenoviral Vectors with Native Tropism Abolished

2002 ◽  
Vol 76 (6) ◽  
pp. 2753-2762 ◽  
Author(s):  
Victor W. van Beusechem ◽  
Jacques Grill ◽  
D. C. Jeroen Mastenbroek ◽  
Thomas J. Wickham ◽  
Peter W. Roelvink ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Brain Tumors ◽  
Human Brain ◽  
Cancer Gene Therapy ◽  
Adenoviral Vectors ◽  
Receptor Expression ◽  
Normal Brain ◽  
Cancer Gene ◽  
Single Chain

ABSTRACT The application of adenoviral vectors in cancer gene therapy is hampered by low receptor expression on tumor cells and high receptor expression on normal epithelial cells. Targeting adenoviral vectors toward tumor cells may improve cancer gene therapy procedures by providing augmented tumor transduction and decreased toxicity to normal tissues. Targeting requires both the complete abolition of native tropism and the addition of a new specific binding ligand onto the viral capsid. Here we accomplished this by using doubly ablated adenoviral vectors, lacking coxsackievirus-adenovirus receptor and αv integrin binding capacities, together with bispecific single-chain antibodies targeted toward human epidermal growth factor receptor (EGFR) or the epithelial cell adhesion molecule. These vectors efficiently and selectively targeted both alternative receptors on the surface of human cancer cells. Targeted doubly ablated adenoviral vectors were also very efficient and specific with primary human tumor specimens. With primary glioma cell cultures, EGFR targeting augmented the median gene transfer efficiency of doubly ablated adenoviral vectors 123-fold. Moreover, EGFR-targeted doubly ablated vectors were selective for human brain tumors versus the surrounding normal brain tissue. They transduced organotypic glioma and meningioma spheroids with efficiencies similar to those of native adenoviral vectors, while exhibiting greater-than-10-fold-reduced background levels on normal brain explants from the same patients. As a result, EGFR-targeted doubly ablated adenoviral vectors had a 5- to 38-fold-improved tumor-to-normal brain targeting index compared to native vectors. Hence, single-chain targeted doubly ablated adenoviral vectors are promising tools for cancer gene therapy. They should provide an improved therapeutic index with efficient tumor transduction and effective protection of normal tissue.

In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors

2004 ◽  
pp. 147-164
Author(s):  
Jean-Noel Gouze ◽  
Martin J. Stoddart ◽  
Elvire Gouze ◽  
Glyn D. Palmer ◽  
Steven C. Ghivizzani ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Synovial Fibroblasts ◽  
Adenoviral Vectors

Enhanced Growth inhibition by Combined Gene Transfer of p53 and p16INK4a in Adenoviral Vectors to Lung Cancer Cell Lines

2001 ◽  
Vol 50 (1) ◽  
pp. 67
Author(s):  
Seung Ho Choi ◽  
Kyung Ho Park ◽  
Ja Young Seol ◽  
Chul Gyu Yoo ◽  
Choon Taek Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Gene Transfer ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Adenoviral Vectors ◽  
Lung Cancer Cell

Budesonide Enhances Repeated Gene Transfer and Expression in the Lung with Adenoviral Vectors

2001 ◽  
Vol 164 (5) ◽  
pp. 866-872 ◽  
Author(s):  
MARTIN KOLB ◽  
MARK INMAN ◽  
PETER J. MARGETTS ◽  
TOM GALT ◽  
JACK GAULDIE
Keyword(s):  
Gene Transfer ◽  
Adenoviral Vectors

Dual-expressing adenoviral vectors encoding the sodium iodide symporter for use in noninvasive radiological imaging of therapeutic gene transfer

2006 ◽  
Vol 33 (3) ◽  
pp. 391-398 ◽  
Author(s):  
Gang Niu ◽  
Richard D. Anderson ◽  
Mark T. Madsen ◽  
Michael M. Graham ◽  
Larry W. Oberley ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Sodium Iodide ◽  
Adenoviral Vectors ◽  
Radiological Imaging ◽  
Sodium Iodide Symporter ◽  
Therapeutic Gene

scholarly journals Vascular Gene Transfer of Phosphomimetic Endothelial Nitric Oxide Synthase (S1177D) Using Ultrasound-Enhanced Destruction of Plasmid-Loaded Microbubbles Improves Vasoreactivity

Circulation ◽  
2002 ◽  
Vol 105 (9) ◽  
pp. 1104-1109 ◽  
Author(s):  
Claudius Teupe ◽  
Sergio Richter ◽  
Beate Fisslthaler ◽  
Voahanginirina Randriamboavonjy ◽  
Christian Ihling ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Gene Transfer ◽  
Endothelial Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Vascular Gene Transfer
Sign in / Sign up

Export Citation Format

Share Document