scholarly journals In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

10.3791/51274 ◽  
2014 ◽  
Author(s):  
Stefanie Fischer ◽  
Christian Engelmann ◽  
Karl-Heinz Herrmann ◽  
Jürgen R. Reichenbach ◽  
Otto W. Witte ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Nerve Fiber ◽  
Optic Nerve Fiber ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri

scholarly journals Noninvasive Visualization of in Vivo Drug Delivery of Poly(l-glutamic acid) Using Contrast-Enhanced MRI

2006 ◽  
Vol 3 (5) ◽  
pp. 507-515 ◽  
Author(s):  
Furong Ye ◽  
Tianyi Ke ◽  
Eun-Kee Jeong ◽  
Xuli Wang ◽  
Yongen Sun ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Glutamic Acid ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri

Dynamic contrast enhanced MRI in the nonhuman primate allows correlation of in vivo placental perfusion with in vitro placental outcomes

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A59-A60
Author(s):  
Victoria Roberts ◽  
Jessica Walker ◽  
Christopher Kroenke ◽  
Matthias Schabel ◽  
Jamie Lo ◽  
...  
Keyword(s):  
Nonhuman Primate ◽  
Placental Perfusion ◽  
Dynamic Contrast Enhanced Mri ◽  
Dynamic Contrast Enhanced ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri

Onset and Maintenance of Angiogenesis in Biomaterials: In Vivo Assessment by Dynamic Contrast-Enhanced MRI

2009 ◽  
Vol 15 (3) ◽  
pp. 455-462 ◽  
Author(s):  
Sebastian Sauerbier ◽  
Moritz Palmowski ◽  
Michael Vogeler ◽  
Heiner Nagursky ◽  
Ali Al-Ahmad ◽  
...  
Keyword(s):  
Dynamic Contrast Enhanced Mri ◽  
Dynamic Contrast Enhanced ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
In Vivo Assessment ◽  
Contrast Enhanced Mri

In vivo measurement of the thalamic subregions using contrast-enhanced MRI

NeuroImage ◽  
2001 ◽  
Vol 13 (6) ◽  
pp. 255
Author(s):  
Ruth Spinks ◽  
Greg Harris ◽  
Nancy C. Andreasen ◽  
Steve Ziebell ◽  
Vincent A. Magnotta ◽  
...  
Keyword(s):  
In Vivo Measurement ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri

scholarly journals A Safe Harbor-Targeted CRISPR/Cas9 Homology Independent Targeted Integration (HITI) System for Multi-Modality Reporter Gene-Based Cell Tracking

2020 ◽  
Author(s):  
John J Kelly ◽  
Moe Saee-Marand ◽  
Nivin N Nyström ◽  
Yuanxin Chen ◽  
Melissa M Evans ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Cell Tracking ◽  
Integration Site ◽  
Reporter Genes ◽  
Safe Harbor ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Targeted Integration ◽  
Contrast Enhanced Mri

AbstractImaging reporter genes can provide valuable, longitudinal information on the biodistribution, growth and survival of engineered cells in preclinical models and patients. A translational bottleneck to using reporter genes in patients is the necessity to engineer cells with randomly-integrating vectors. CRISPR/Cas9 targeted knock-in of reporter genes at a genomic safe harbor locus such as adeno-associated virus integration site 1 (AAVS1) may overcome these safety concerns. Here, we built Homology Independent Targeted Integration (HITI) CRISPR/Cas9 minicircle donors for precise AAVS1-targeted simultaneous knock-in of fluorescence, bioluminescence, and MRI (Oatp1a1) reporter genes. Our results showed greater knock-in efficiency at the AAVS1 site using HITI vectors compared to homology-directed repair donor vectors. Characterization of select HITI clones demonstrated functional fluorescence and bioluminescence reporter activity as well as significantly increased Oatp1a1-mediated uptake of the clinically-approved MRI agent gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid. As few as 106 Oatp1a1-expressing cells in a 50 µl subcutaneous injection could be detected in vivo with contrast-enhanced MRI. Contrast-enhanced MRI also improved the conspicuity of both sub-cutaneous and metastatic Oatp1a1-expressing tumours prior to them being palpable or even readily visible on pre-contrast images. Our work demonstrates the first CRISPR/Cas9 HITI system for knock-in of large DNA donor constructs at a safe harbor locus, enabling multi-modal longitudinal in vivo imaging of cells. This work lays the foundation for safer, non-viral reporter gene tracking of multiple cell types.

scholarly journals Dynamic contrast-enhanced MRI of Gd-albumin delivery to the rat hippocampus in vivo by convection-enhanced delivery

2012 ◽  
Vol 209 (1) ◽  
pp. 62-73 ◽  
Author(s):  
Jung Hwan Kim ◽  
Garrett W. Astary ◽  
Tatiana L. Nobrega ◽  
Svetlana Kantorovich ◽  
Paul R. Carney ◽  
...  
Keyword(s):  
Rat Hippocampus ◽  
Convection Enhanced Delivery ◽  
Dynamic Contrast Enhanced Mri ◽  
Dynamic Contrast Enhanced ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri
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