scholarly journals Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

10.3791/51044 ◽  
2014 ◽  
Author(s):  
William S. Turner ◽  
Nabjot Sandhu ◽  
Kara E. McCloskey
Keyword(s):  
Tissue Engineering ◽  
3D Scaffold ◽  
Cell Sheets ◽  
Engineering Construction

scholarly journals Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

2019 ◽  
Vol 20 (22) ◽  
pp. 5656 ◽  
Author(s):  
Min-Ah Koo ◽  
Mi Hee Lee ◽  
Jong-Chul Park
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cell Detachment ◽  
Oxygen Species ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
New Approach ◽  
Responsive Systems ◽  
Cell Based Therapy ◽  
Responsive Cell

Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

scholarly journals Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Claudio Muscari ◽  
Emanuele Giordano ◽  
Francesca Bonafè ◽  
Marco Govoni ◽  
Carlo Guarnieri
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Future Research ◽  
Myocardial Tissue ◽  
Cell Sheets ◽  
Mixed Cell ◽  
Differentiation Potential ◽  
Cardiac Wall ◽  
Basic Approaches ◽  
Myocardial Tissue Engineering

The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. In addition to these basic approaches, more sophisticated strategies have been followed employing mixed-cell sheets, microvascular modules, and inosculation from vascular explants. Technologies exerting spatial control of vascular cells, such as topographical surface roughening and ordered patterning, represent other ways to drive scaffold vascularization. Finally, microfluidic devices and bioreactors exerting mechanical stress have also been employed for high-throughput scaling-up production in order to accelerate muscle differentiation and speeding the endothelialization process. Future research should address issues such as how to optimize cells, biomaterials, and biochemical components to improve the vascular integration of the construct within the cardiac wall, satisfying the metabolic and functional needs of the myocardial tissue.

Tissue Engineering: Biomimetic Concealing of PLGA Microspheres in a 3D Scaffold to Prevent Macrophage Uptake (Small 11/2016)

Small ◽  
2016 ◽  
Vol 12 (11) ◽  
pp. 1394-1394
Author(s):  
Silvia Minardi ◽  
Bruna Corradetti ◽  
Francesca Taraballi ◽  
Monica Sandri ◽  
Jonathan O. Martinez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Plga Microspheres ◽  
3D Scaffold ◽  
Macrophage Uptake
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